激素和非生物胁迫下荷花GH3基因家族的表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2024-0704

摘要/Abstract

摘要：

目的探究荷花（Nelumbo nucifera）NnGH3家族成员的特征及其在非生物胁迫响应中的作用，为荷花抗逆新品种的选育提供新的关键候选基因资源。方法采用生物信息学方法从荷花全基因组中鉴定酰基酰胺合成酶（GH3）家族成员，对GH3基因编码的酰胺合成酶理化性质、染色体定位、三级结构预测、系统发育树、基因结构、共线性和表达模式等进行详细研究，并利用RT-qPCR技术探究NnGH3家族基因分别在低温（4℃）、厌氧（水淹）、盐害（300 mmol/L NaCl）及外源施加激素（0.1 mmol/L IAA、1 mmol/L JA和5 mmol/L SA）处理下的表达模式。结果从荷花基因组中鉴定出14个NnGH3家族成员，它们集中分布在5条染色体上，根据其在染色体的位置依次命名为NnGH3.1-3.14，其分子质量为365-630 aa，理论等电点均小于7，为酸性蛋白，亚细胞定位于细胞核和细胞质上；聚类分析表明GH3家族成员分为Group Ⅰ和Group Ⅱ两组，绝大多数成员含有3-4个外显子和GH3 superfamily结构域。14个NnGH3基因中有2对基因存在共线性，与拟南芥（Arabidopsis thaliana）、水稻（Oryza sativa）GH3基因分别存在14和6对共线关系。顺式作用元件预测分析表明，该家族基因存在大量光、逆境和激素等响应元件。表达分析结果显示，NnGH3负向响应低温胁迫，绝大多数成员正向响应SA处理和盐胁迫，特异性响应IAA、JA处理及厌氧胁迫，其中Group Ⅱ中的NnGH3.4和NnGH3.13基因以及Group Ⅰ中的NnGH3.3基因在所有处理中表达量变化最为显著。结论在全基因组范围内从荷花中系统鉴定得到14个NnGH3家族成员，分为Group Ⅰ和Group Ⅱ两组，均含有GH3 superfamily结构域，为酸性蛋白。NnGH3家族基因在不同的外源激素和非生物胁迫条件下的表达具有特异性。

关键词: 荷花, GH3基因家族, 生物信息学, 植物激素, 非生物胁迫, 表达模式

Abstract:

Objective To characterize NnGH3 gene family in lotus (Nelumbo nucifera) and analyze their roles in abiotic stress, and to provide key candidate genetic resources for the breeding of new lotus varieties with stress resistance. Method Bioinformatics methods were used to identify the family members of acyl amides synthetase (GH3) from the whole genome of N. nucifera, and to conduct the detailed study on the phytochemical properties, chromosomal localization, tertiary structure prediction, systematic evolution, gene structure, collinearity relationships, and expression patterns of GH3 enzymes. RT-qPCR technology was applied to investigate the expression patterns of NnGH3 family genes under various stress conditions including low temperature(4℃), anaerobic conditions(submergence), salt stress(300 mmol/L NaCl), and exogenous hormone treatments(0.1 mmol/L IAA, 1 mmol/L JA, and 5 mmol/L SA). Result The fourteen NnGH3 family members were identified from the lotus genome, distributed on 5 chromosomes, and named sequentially as NnGH3.1 to NnGH3.14 based on their chromosomal locations. Their molecular weights ranged from 365 to 630 amino acids, theoretical isoelectric points were all less than 7, and acidic proteins were localized in the nucleus and cytoplasm. Cluster analysis revealed two groups, Group I and Group Ⅱ, within the GH3 family, with most members containing 3-4 exons and GH3 superfamily domains. Collinearity analysis indicated that 2 pairs of genes among the 14 lotus GH3 genes, 14 pairs with (Arabidopsis thaliana) GH3 genes, and 6 pairs with (Oryza sativa) GH3 genes presented collinear relationships. Promoter cis-acting element analysis showed a lot of light-, stress-, and hormone-responsive elements within this family. Expression analysis demonstrated that NnGH3 genes negatively responded to low temperature stress, with most members positively responded to SA treatments and salt stress, and specifically responded to IAA and JA treatments as well as anaerobic stress. Notably,genes NnGH3.4 and NnGH3.13 from Group Ⅱ, and NnGH3.3 from Group I, had the most significant expression changes across all treatments. Conclusion Fourteen NnGH3 family members are identified and divided into two groups, Group Ⅰ and Group Ⅱ, which all contain GH3 superfamily domains and are acidic proteins. NnGH3 family genes are specifically expressed under different exogenous hormone and abiotic stress conditions.

Key words: Nelumbo nucifera, GH3 gene family, bioinformatics, phytohormone, abiotic stresses, expression pattern

匡健华, 程志鹏, 赵永晶, 杨洁, 陈润乔, 陈龙清, 胡慧贞. 激素和非生物胁迫下荷花GH3基因家族的表达分析[J]. 生物技术通报, 2025, 41(2): 221-233.

KUANG Jian-hua, CHENG Zhi-peng, ZHAO Yong-jing, YANG Jie, CHEN Run-qiao, CHEN Long-qing, HU Hui-zhen. Expression Analysis of the GH3 Gene Family in Nelumbo nucifera underHormonal and Abiotic Stresses[J]. Biotechnology Bulletin, 2025, 41(2): 221-233.

图/表 10

表1 引物序列表

Table 1 Sequences of primers

序号 No.	基因名称 Gene name	正向引物 Forward primer （5′-3′）	反向引物 Reverse primer （5′-3′）
1	NnGH3.1	TCACTCAGTTCTGGCACCAC	TGCAAGACCCCCTTTCGTTT
2	NnGH3.2	GGCAACCCATGACTACGTGA	CTGTACGCCCATTAAGCCCA
3	NnGH3.3	GATGGTTCGAAGAGGTCGCA	GAGGTGTACAGGGCTTCCAG
4	NnGH3.4	CTCACGCCTGTGATGAACCT	CTGGTCTTGAAGTGGTCGCT
5	NnGH3.5	GGTTCGAAGAGGTGGCAGAA	AATCCGCATGGGAAGCAAGA
6	NnGH3.6	AGCGCCCATGTCGAATACTT	GGGTAATCCGCAGAGGATGG
7	NnGH3.7	CTAACGGGGAGGCATCAAGG	GACAGGCACCAGCAGGTTAT
8	NnGH3.8	CCTGACTTCCGCCAATCCTT	CTAGTTCAGGGTTGGGCCTG
9	NnGH3.9	GTCTGGACCAAGGGAAAGGG	CTCGCTGTACCAGACCACAA
10	NnGH3.10	GTACAAAGGGGAGGAGGCAC	GACGCTTGAACGTTGTCACC
11	NnGH3.11	ACTACGGAAGCTGGAGGACT	GGGCGTAGTCGTCGTAAGAG
12	NnGH3.12	CCTCTTGCTTCCCATGCAGA	ACCCTTGATCTGTAAGCGGC
13	NnGH3.13	AGTTCTGGAACTTCTGCGGG	ACCTGGCGTCTTTGTCTCTG
14	NnGH3.14	AGCCCCCATATGATCGTTGC	GATCGGTTCTGACTCAGCGT
15	NnACT	CTCCGTGTTGCCCCTGAAG	CCAGCAAGGTCCAACCGAAG

图1 GH3基因家族成员在荷花中的染色体定位

Fig. 1 Chromosomal localization of the GH3 gene family members in N. nucifera

表2 荷花NnGH3基因家族二级结构预测

Table 2 Prediction of secondary structure of N. nuciferaNnGH3 gene family

基因名称 Gene name	基因ID Gene identifier	氨基酸数目 Amino acid amount	α螺旋 Alpha helix （Hh）/%	无规则卷曲Random coil （Cc）/%	延伸链 Extended strand （Ee）/%
NnGH3.1	Nn1g01814.2	586	34.64	57.85	7.51
NnGH3.2	Nn1g03776.1	621	34.14	60.39	5.48
NnGH3.3	Nn1g03969.3	590	32.88	59.49	7.63
NnGH3.4	Nn1g04653.2	599	36.06	58.76	5.18
NnGH3.5	Nn1g06683.3	595	34.62	55.63	9.75
NnGH3.6	Nn1g06894.2	616	36.04	59.25	4.71
NnGH3.7	Nn3g16244.1	630	40.48	54.60	4.92
NnGH3.8	Nn3g19075.2	587	34.58	57.24	8.18
NnGH3.9	Nn4g22107.1	615	36.91	57.24	5.85
NnGH3.10	Nn4g25867.1	603	36.15	58.71	5.14
NnGH3.11	Nn4g26117.1	579	38.51	53.71	7.77
NnGH3.12	Nn5g29859.2	592	29.73	59.63	10.64
NnGH3.13	Nn5g30464.6	595	34.45	60.84	4.71
NnGH3.14	Nn7g36856.1	365	22.78	58.89	18.33

图2 荷花GH3基因家族成员编码蛋白的三级结构预测

Fig. 2 Tertiary structure of proteins encoded by GH3 gene family members of N. nucifera

图3 荷花（14个）、拟南芥（19个）和水稻（13个）GH3 基因家族系统发育树代表NnGH3s：荷花；代表AtGH3s：拟南芥；代表OsGH3s：水稻

Fig. 3 Unrooted phylogenetic tree of the GH3 gene family in N. nucifera(14), A. thaliana(19) and O. sativa(13)indicates NnGH3s: Nelumbo nucifera; indicates AtGH3s: Arabidopsis thaliana; indicates OsGH3s: Oryza sativa

图4 荷花NnGH3家族的基序及基因结构

Fig. 4 Motif and gene structure of the NnGH3 family in N. nucifera

图5 NnGH3家族的种内共线性分析（A）和荷花、拟南芥、水稻的种间共线性分析（B）Chr1-Chr8代表荷花1-8号染色体，热图代表基因密度，红线条代表NnGH3基因的组内及种间共线性基因对

Fig. 5 Intraspecific covariance analysis of the NnGH3 family (A) and interspecific covariance analysis (B) of N. nucifera, A. thaliana, and O. sativaChr1-Chr8 indicate chromosome 1-8 of N. nucifera, the heat map indicates gene density, red lines indicate intragroup and interspecies covariate gene pairs for the NnGH3 gene

表3 荷花 GH3 基因家族共线基因对的 Ka/Ks 值

Table 3 Ka/Ks values of collinear gene pairs of GH3 gene family in N. nucifera

基因对

Gene pair

非同义替换率 Non-synonymous substitution rate （Ka）

同义替换率 Synonymous substitution rate （Ks）

Ka/Ks

图6 荷花NnGH3基因家族的启动子顺式作用元件分析

Fig. 6 Cis-acting elements analysis in the promotors of NnGH3 gene family in N. nucifera

图 7 荷花在外源0.1 mmol/L IAA、5 mmol/L SA、1 mmol/L JA及低温（4℃）、水淹、300 mmol/L NaCl处理6 h后NnGH3家族基因的表达分析*：P<0.05，**：P<0.01表示基因的表达量与对照间差异显著

Fig. 7 Expression analysis of the NnGH3 gene family in N. nucifera treated with exogenous 0.1 mmol/L IAA, 5 mmol/L SA, 1 mmol/L JA, and (4℃) low temperature, flooding, 300 mmol/L NaCl for 6 h* P<0.05, **: P<0.01, indicates that the expression of the gene is significantly different from the control

参考文献 39

1	Chapman EJ, Estelle M. Mechanism of auxin-regulated gene expression in plants [J]. Annu Rev Genet, 2009, 43: 265-285.
2	余姝姝, 周俊琴, 卢梦琪, 等.油茶3个 ARF 基因的克隆及表达分析 [J] .植物生理学报, 2021, 57(5): 1151-1162.
	Yu SS, Zhou JQ, Lu MQ, et al. Cloning and expression analysis of three ARF genes in Camellia oleifera [J] . Plant Physiol J, 2021, 57(5): 1151-1162.
3	Park JE, Park JY, Kim YS, et al. GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis [J]. J Biol Chem, 2007, 282(13): 10036-10046.
4	Hagen G, Kleinschmidt A, Guilfoyle T. Auxin-regulated gene expression in intact soybean hypocotyl and excised hypocotyl sections [J]. Planta, 1984, 162(2): 147-153.
5	Conner TW, Goekjian VH, LaFayette PR, et al. Structure and expression of two auxin-inducible genes from Arabidopsis [J]. Plant Mol Biol, 1990, 15(4): 623-632.
6	Staswick PE, Serban B, Rowe M, et al. Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid [J]. Plant Cell, 2005, 17(2): 616-627.
7	Terol J, Domingo C, Talón M. The GH3 family in plants: genome wide analysis in rice and evolutionary history based on EST analysis [J]. Gene, 2006, 371(2): 279-290.
8	Tuskan GA, Difazio S, Jansson S, et al. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray) [J]. Science, 2006, 313(5793): 1596-1604.
9	Kumar R, Agarwal P, Tyagi AK, et al. Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum) [J]. Mol Genet Genomics, 2012, 287(3): 221-235.
10	Yuan HZ, Zhao K, Lei HJ, et al. Genome-wide analysis of the GH3 family in apple (Malus × domestica) [J]. BMC Genomics, 2013, 14: 297.
11	侯黔东, 沈天娇, 余欢欢, 等. 甜樱桃GH3基因家族全基因组鉴定与表达分析 [J]. 园艺学报, 2021, 48(12): 2360-2374.
	Hou QD, Shen TJ, Yu HH, et al. Genome-wide identification and expression analysis of Prunus avium gretchen Hagen 3 (GH3) gene family [J]. Acta Hortic Sin, 2021, 48(12): 2360-2374.
12	Hagen G, Guilfoyle T. Auxin-responsive gene expression: genes, promoters and regulatory factors [J]. Plant Mol Biol, 2002, 49(3/4): 373-385.
13	Sherp AM, Westfall CS, Alvarez S, et al. Arabidopsis thaliana GH3.15 acyl acid amido synthetase has a highly specific substrate preference for the auxin precursor indole-3-butyric acid [J]. J Biol Chem, 2018, 293(12): 4277-4288.
14	园园, 恩和巴雅尔, 齐艳华. 植物GH3基因家族生物学功能研究进展 [J]. 植物学报, 2023, 58(5): 770-782.
	Yuan Y, En HBYE, Qi YH. Research advances in biological functions of GH3 gene family in plants [J]. Chin Bull Bot, 2023, 58(5): 770-782.
15	Hui SG, Hao MY, Liu HB, et al. The group I GH3 family genes encoding JA-Ile synthetase act as positive regulator in the resistance of rice to Xanthomonas oryzae pv. oryzae [J]. Biochem Biophys Res Commun, 2019, 508(4): 1062-1066.
16	Rao MV, Lee H, Creelman RA, et al. Jasmonic acid signaling modulates ozone-induced hypersensitive cell death [J]. Plant Cell, 2000, 12(9): 1633-1646.
17	Jagadeeswaran G, Raina S, Acharya BR, et al. Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae [J]. Plant J, 2007, 51(2): 234-246.
18	周淑瑶, 李建明, 毛娟. AtGH3.17调控拟南芥生长素和油菜素甾醇的响应 [J]. 植物学报, 2023, 58(3): 373-384.
	Zhou SY, Li JM, Mao J. AtGH3.17-mediated regulation of auxin and brassinosteroid response in Arabidopsis thaliana [J]. Chin Bull Bot, 2023, 58(3): 373-384.
19	曾亚, 丁新华, 沈祥陵, 等. 水稻抗病基因介导的抗白叶枯病反应中蛋白质表达谱的比较分析 [J]. 中国水稻科学, 2008, 22(3): 234-242.
	Zeng Y, Ding XH, Shen XL, et al. Analysis of protein expression profiling in rice disease resistance gene-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Chin J Rice Sci, 2008, 22(3): 234-242.
20	Ding XH, Cao YL, Huang LL, et al. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate- and jasmonate-independent basal immunity in rice [J]. Plant Cell, 2008, 20(1): 228-240.
21	Wang SK, Bai YH, Shen CJ, et al. Auxin-related gene families in abiotic stress response in Sorghum bicolor [J]. Funct Integr Genom, 2010, 10(4): 533-546.
22	Singh VK, Jain M, Garg R. Genome-wide analysis and expression profiling suggest diverse roles of GH3 genes during development and abiotic stress responses in legumes [J]. Front Plant Sci, 2015, 5: 789.
23	王其超, 张行言.荷花发展前景——从中国视角展望 [J] .中国园林, 2011, 27(1): 50-53.
	Wang QC, Zhang XY. The development prospect of Nelumbo nucifera-from the perspective of China [J] .Chin Landsc Archit, 2011, 27(1): 50-53.
24	程志鹏, 汪仲毅, 匡健华, 等. 荷花PIN基因家族的鉴定及非生物胁迫表达分析 [J]. 西北植物学报, 2024, 44(1): 1-12.
	Cheng ZP, Wang ZY, Kuang JH, et al. Genome-wide identification and expression pattern of the PIN gene family under abiotic stress in Nelumbo nucifera [J]. Acta Bot Boreali Occidentalia Sin, 2024, 44(1): 1-12.
25	李祥志, 刘兆磊, 陈发棣, 等. 荷花耐深水评价体系及耐深水鉴定 [J]. 安徽农业科学, 2014, 42(3): 679-682.
	Li XZ, Liu ZL, Chen FD, et al. Study on establishment of evaluation system for deepwater tolerance and its identification of Nelumbo nucifera gaertn [J]. J Anhui Agric Sci, 2014, 42(3): 679-682.
26	汪仲毅, 程志鹏, 顾伟卓, 等. 荷花(Nelumbo nucifera)全基因组PRR基因家族鉴定及其在多种非生物胁迫下的表达模式 [J]. 基因组学与应用生物学, 2023, 42(1): 60-72.
	Wang ZY, Cheng ZP, Gu WZ, et al. Genome-wide identification and expression pattern of PRR gene family in Nelumbo nucifera under various abiotic stresses [J]. Genom Appl Biol, 2023, 42(1): 60-72.
27	Zou WH, Lin PX, Zhao ZN, et al. Genome-wide identification of auxin-responsive GH3 gene family in Saccharum and the expression of ScGH3-1 in stress response [J]. Int J Mol Sci, 2022, 23(21): 12750.
28	Pinto RT, Freitas NC, Máximo WPF, et al. Genome-wide analysis, transcription factor network approach and gene expression profile of GH3 genes over early somatic embryogenesis in Coffea spp [J]. BMC Genomics, 2019, 20(1): 812.
29	Yang YJ, Yue RQ, Sun T, et al. Genome-wide identification, expression analysis of GH3 family genes in Medicago truncatula under stress-related hormones and Sinorhizobium meliloti infection [J]. Appl Microbiol Biotechnol, 2015, 99(2): 841-854.
30	Feng L, Li GR, He ZB, et al. The ARF, GH3, and Aux/IAA gene families in castor bean (Ricinus communis L.): Genome-wide identification and expression profiles in high-stalk and dwarf strains [J]. Ind Crops Prod, 2019, 141: 111804.
31	林晓艺, 唐梦洁, 李小芳, 等. 龙眼GH3家族成员的全基因组鉴定及表达分析 [J]. 福建农林大学学报: 自然科学版, 2023, 52(2): 166-177.
	Lin XY, Tang MJ, Li XF, et al. Genome-wide identification and expression analysis of GH3 gene family in Dimocarpus longan [J]. J Fujian Agric For Univ Nat Sci Ed, 2023, 52(2): 166-177.
32	岳龙, 罗振兴, 张永鑫, 等. 梨PbrGH3基因家族的鉴定与分析 [J]. 分子植物育种, 2024, 22(7): 2137-2143.
	Yue L, Luo ZX, Zhang YX, et al. Identification and analysis of the PbrGH3 gene family in Pyrus bretschneideri [J]. Mol Plant Breed, 2024, 22(7): 2137-2143.
33	邢媛, 宋健, 李俊怡, 等. 谷子AP基因家族鉴定及其对非生物胁迫的响应分析 [J]. 生物技术通报, 2023, 39(11): 238-251.
	Xing Y, Song J, Li JY, et al. Identification of AP gene family and its response analysis to abiotic stress in Setaria italica [J]. Biotechnol Bull, 2023, 39(11): 238-251.
34	陈凯, 佟晓楠, 张晓媛, 等. 枳LEA基因家族鉴定及其对非生物胁迫的响应 [J]. 西北植物学报, 2023, 43(6): 918-928.
	Chen K, Tong XN, Zhang XY, et al. Genome-wide identification and abiotic stress responses of LEA gene family in Poncirus trifoliata [J]. Acta Bot Boreali Occidentalia Sin, 2023, 43(6): 918-928.
35	Yan SP, Dong XN. Perception of the plant immune signal salicylic acid [J]. Curr Opin Plant Biol, 2014, 20: 64-68.
36	Li JY, Min XY, Luo K, et al. Molecular characterization of the GH3 family in alfalfa under abiotic stress [J]. Gene, 2023, 851: 146982.
37	Vijayan P, Shockey J, Lévesque CA, et al. A role for jasmonate in pathogen defense of Arabidopsis [J]. Proc Natl Acad Sci U S A, 1998, 95(12): 7209-7214.
38	Du H, Wu N, Fu J, et al. A GH3 family member, OsGH3-2, modulates auxin and abscisic acid levels and differentially affects drought and cold tolerance in rice [J]. J Exp Bot, 2012, 63(18): 6467-6480.
39	Westfall CS, Zubieta C, Herrmann J, et al. Structural basis for prereceptor modulation of plant hormones by GH3 proteins [J]. Science, 2012, 336(6089): 1708-1711.

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