人酸性成纤维细胞生长因子的研究进展

生物技术通报 ›› 2013, Vol. 0 ›› Issue (5): 22-27.

人酸性成纤维细胞生长因子的研究进展

谭亚清, 刘德虎

(中国农业科学院生物技术研究所，北京 100081)

收稿日期:2012-11-22 修回日期:2013-05-24 出版日期:2013-05-24 发布日期:2013-05-24
作者简介:谭亚清，女，硕士研究生，研究方向：植物分子生物学与基因工程；E-mail：tanyaqin.g@163.com
基金资助:
中国农业科学院生物技术研究所中央级公益性科研院所基本科研业务专项资助

The Prospect and Development of the Human Acid Fibroblast Growth Factor

Tan Yaqing, Liu Dehu

(Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081)

Received:2012-11-22 Revised:2013-05-24 Published:2013-05-24 Online:2013-05-24
About author:刘德虎，男，博士，研究员，研究方向：植物基因工程和植物生物反应器；E-mail：liudehu@public3.bta.net.cn

摘要/Abstract

摘要： 成纤维细胞生长因子是一个大的蛋白质家族，人酸性成纤维细胞生长因子(human acidic fibroblast growth factor，haFGF)是其中的重要一员，具有广泛的生物学活性和潜在的临床应用价值。概述haFGF的结构、功能及其在基因工程方面的研究进展，并对haFGF在临床应用中可能存在的问题进行简单的探讨。

关键词: 人酸性成纤维细胞生长因子, 理化结构, 生物学功能, 临床应用, 基因工程

Abstract: Fibroblast growth factor is a large protein family, human acidic fibroblast growth factor is an important member of it, which has extensive biological functions and potential clinical value. In this paper we review the progress in the study on the structure, function and genetic engineering of human acidic fibroblast growth factor. Some prospects and problems about its application are also discussed.

Key words: Human acidic fibroblast growth factor, Physical and chemical structure, Biological function, Clinical application, Genetic engineering

谭亚清, 刘德虎. 人酸性成纤维细胞生长因子的研究进展[J]. 生物技术通报, 2013, 0(5): 22-27.

Tan Yaqing, Liu Dehu. The Prospect and Development of the Human Acid Fibroblast Growth Factor[J]. Biotechnology Bulletin, 2013, 0(5): 22-27.

参考文献

[1] Gospodarowicz D, Jones KL, Sato G. Purification of a growth factor for ovarian cells from bovine pituitary glands[J]. Proc Natl Acad Sci USA, 1974, 71(6) ：2295-2299.
[2] Thomas KA, Rios-Candelore M, Fitzpatrick S. Purification and characterization of acidic fibroblast growth factor from bovine brain[J]. Proc Natl Acad Sci USA, 1984, 81：357-361.
[3] Mohan SK, Rani SG, Yu C. The heterohexameric complex structure, a component in the non-classical pathway for fibroblast growth factor 1 (FGF1) secretion[J]. J Biol Chem, 2010, 285(20)：15464-15475.
[4] Chi YH, Kumar TK, Chiu IM, et al. Identification of rare partially unfolded states in equilibrium with native conformation in an all beta-barrel protein[J]. J Biol Chem, 2002, 277(38)：34941-34948.
[5] Guzman-Casado M, Cardente A, Gimenea-Gallego G, et al. Myo-inositol hexasulphate and low moleculer weight heparin binding to human acidic fibroblast growth factor：a calorimetric and FTIR study[J]. Int J Biol Macrom, 2001, 28(4)：305-313.
[6] Margalit H, Fischer N, Ben-Sasson SA, et al. Comparative analysis of structurally defined heparin binding sequences reveals a distinct spatial distribution of basic residues[J]. J Biol Chem, 1993, 268(26)：19228-19231.
[7] Zakrzewska M, Krowarsch D, Wiedlocha A, et al. Structural require-ments of FGF-1 for receptor binding and translocation into cells[J]. Biochemistry, 2006, 45(51)：15338-15348.
[8] Stauber DJ, Digabriele AD, Hendrickson WA. Structural interaction of fibroblast growth factor recepter with its ligands[J]. Proc Natl Acad Sci USA, 2000, 97(1) ：49-54.
[9] Lozano RM, Pineda-Lucena A, Gonzalez C, et al. 1H NMR structural characterization of a nonmitogenic, vasodilatory, ischemia-protector and neuromodulatory acidic fibroblast growth factor[J]. Bioche-mistry, 2000, 39(17)：4982-4993.
[10] Lin YZ, Yao SY, Hawiger J. Role of nuclear localization sequence in fibroblast growth facter-1-stimulated mitogenic pathways[J]. J Biol Chem, 1996, 271(10) ：5305-5308.
[11] Fu YM, Spirito P, Yu ZX, et al. Acidic fibroblast growth factor in the developing rat embryo [J]. J Cell Biol, 1991, 114(6)：1261-1273.
[12] Isaacs HV. New perspectives on the role of the fibroblast growth factor family in amphibian development[J]. Cell Mol Life Sci, 1997, 53(4)：350-361.
[13] 章斌, 梅举, 张宝仁, 等. 酸性成纤维细胞生长因子促血管内皮细胞增殖作用的实验研究[J]. 皖南医学院学报, 2003, 22(1)：18-19.
[14] Ferrara N. Vascular endothelial growth factor and the regulation of angiogenesis[J]. Recent Prog Horm Res, 2000, 55：15-35.
[15] 刘炘, 马嵘, 马珂, 等. 重组人酸性成纤维细胞生长因子对创面愈合的作用[J]. 中国药科大学学报, 2008, 39(1)：87-91.
[16] 翁立新, 付小兵, 李秀霞, 等. 改构型和野生型aFGF对肠缺血—再灌注损伤后肝肾功能的影响[J]. 中国危重病急救医学, 2004, 16(1)：19-21.
[17] Buehler A, Martire A, Strohm C, et al. Angiogenesis-independent cardioprotection in FGF-1 transgenic mice [J]. Cardiovasc Res, 2002, 55(4)：768-777.
[18] Li AJ, Oomura Y, Sasaki K, et al. A single pre-training glucose injection induces memory fac ilitation in rodents performing various tasks：contribution of acidic fibroblast growth factor[J]. Neuroscience, 1998, 85(3) ：785-794.
[19] Lipton SA, Wagner JA, Madison RD, et al. Acidic fibroblast growth factor enhances regeneration of processes by postnatal mammalian retinal ganglion cells in culture [J]. Proc Natl Acad Sci USA, 1988, 85(7)：2388-2392.
[20] Lee LM, Huang MC, Chuang TY, et al. Acidic FGF enhances functional regeneration of adult dorsal roots[J]. Life Sci, 2004, 74(15)：1937-1943.
[21] Wu H, Yao P, Liu N, et al. Prevention of osteoarthritis cartilage degeneration with intraarticular injection of recombinant human acidic fibroblast growth factor[J]. J Clin Rehab Tissue Eng Res, 2007, 11(32)：6394-6396.
[22] 陈纪宁, 屠重棋, 龚全, 等. 酸性成纤维细胞生长因子促进引导性骨再生的实验研究[J]. 中国修复重建外科杂志, 1999, 13(5)：309-314.
[23] Jaye M, Howk R, Burgess W, et al. Human endothelial cell growth factor：cloning, nucleotide sequence, and chromosome localization[J]. Science, 1986, 233(4763)：541-545.
[24] Zazo M, Lozano RM, Ortega S, et al. High-level synthesis in Escherichia coli of shortened and full-length human acidic fibroblast growth factor and purification in a form stable in aqueous solutions [J]. Gene, 1992, 113(2)：231-238.
[25] 汪浩勇, 王会倍, 黄培堂, 等. 人酸性成纤维细胞生长因子基因在大肠杆菌中的克隆和表达[J]. 生物化学杂志, 1991, 7(6)：713-718.
[26] Cao YH, Pettersson RF. Human acidic fibroblast growth factor overexpressed in insect cells is not secreted into the medium[J]. Growth Factors, 1990, 3(1)：1-13.
[27] Wu X, Kamei K, Sato H, et al. High-level expression of human acidic fibroblast growth factor and basic fibroblast growth factor in silkworm (Bombyx mori L.) using recombinant baculovirus[J]. Protein Expr and Purif, 2001, 21(1)：192-200.
[28] Fantoni A, Bill RM, Gustafsson L, et al. Improved yields of full-length functional human FGF1 can be achieved using the methylotrophic yeast Pichia pastoris[J]. Protein Expr Purif, 2007, 52(1)：31-39.
[29] Zhou Y, Ren LZ, Zhu JG, et al. Construction of a recombinant human FGF1 expression vector for mammary gland-specific expression in human breast cancer cells[J]. Mol Cell Biochem, 2011, 354 (1-2)：39-46.
[30] Fan YJ, Li W, Wang JJ, et al. Efficient production of human acidic fibroblast growth factor in pea (Pisum sativum L.) plants by agroi-nfection of germinated seeds[J]. BMC Biotechnol, 2011, 11：45.
[31] Hull AK, Criscuolo CJ, Mett V, et al. Human-derived, plant-produced monoclonal antibody for the treatment of anthrax[J]. Vaccine, 2005, 23(17-18)：2082-2086.
[32] 王通, 李校堃. 生长因子的新面孔——国家十五重大科技攻关项目概览[J]. 中国处方药, 2003, 2(2)：27-29.
[33] 李校堃, 许华, 赵文, 等. 重组人酸性成纤维细胞生长因子皮肤用药的药代动力学[J]. 药学学报, 2002, 37(6) ：424- 427.
[34] 许华, 李校, 郑青, 等. 重组人酸性成纤维细胞生长因子皮肤用药的长期毒性研究[J]. 中国药科大学学报, 2003, 34(1)：46-50.
[35] Li HH, Fu XB, Sun TZ, et al. Non-mitogenic acidic fibroblast growth factor reduces intestinal dysfunction induced by ischemia and reperfusion injury in rats[J]. J Gastroenterol Hepatol, 2007, 22(3)：363-370.
[36] Ma B, Cheng DS, Xia ZF, et al. Randomized, multicenter, double-blind, and placebo-controlled trial using topical recombinant human acidic fibroblast growth factor for deep partial-thickness burns and skin graft donor site[J]. Would Repair Regen, 2007, 15(6)：795-799.
[37] 刘华伟, 赵勇, 李贵生, 等. FGF-1及其突变体的研究进展[J]. 中国生物工程杂志, 2005, 25(8) ：1-5.