植物中的钙依赖蛋白激酶(CDPK)的结构特征和功能研究进展

摘要/Abstract

摘要： Ca²⁺是植物中重要的第二信使, 细胞中的钙信号经钙传感蛋白(CaMs、CaMLs、CBLs和CDPKs) 传递到下游组分(转录因子, NADPH氧化酶基因等) , 引起相关基因的表达, 使植物对生物或非生物胁迫作出响应。钙依赖蛋白激酶(CDPKs) 是在植物及原生动物中发现的丝氨酸/苏氨酸蛋白激酶, 在Ca²⁺介导的信号途径中起重要作用。CDPKs是由多基因家族编码, 分属4个亚家族, 各亚族成员具有相同或不同的表达模式、亚细胞定位、底物特异性、功能各异或冗余等特性。综述CDPKs的结构特征、表达模式、定位、调控、体内外底物、抑制剂, 以及在响应生物及非生物胁迫中的作用等方面的研究进展, 旨在探讨CDPKs的功能及其调控机制。

关键词: 钙依赖蛋白激酶(CDPKs), 结构特征, 调控机制, 生物学功能

Abstract: The calcium ion(Ca²⁺)is known as a key second messenger in plants, intracellular Ca²⁺ signals are relayed to downstream(transcription factors, NADPH oxidases genes)via different calcium sensor proteins(CaMs, CaMLs, CBLs, CDPKs), which further causes expression of related genes and responses to abiotic and biotic stresses. Calcium-dependent protein kinases(CDPKs)which are Ser/The protein kinases found in plants and some protozoa, play crucial roles in Ca²⁺-mediated signaling pathways. CDPKs are encoded by multigene families and are divided into four subgroups. CDPKs exhibit overlapping and distinct expression patterns, sub-cellular localizations, substrate specificities and redundancy and/or diversity functions. Here we review the recent advances on the structural characteristics, expression patterns, localizations, regulations substrates both in vivo and in vitro, inhibitors and functions in response to abiotic and biotic stresses of CDPKs in order to shed light on the functions and regulatory mechanisms.

Key words: Calcium-dependent protein kinases(CDPKs), Structural characteristics, Regulatory mechanism, Biological functions

姜珊珊, 张丹, 孔祥培, 周严, 李德全. 植物中的钙依赖蛋白激酶(CDPK)的结构特征和功能研究进展[J]. 生物技术通报, 2013, 0(6): 12-19.

Jiang Shanshan, Zhang Dan, Kong Xiangpei, Zhou Yan, Li Dequan. Research Progress of Structural Characteristics and Functions of Calcium-dependent Protein Kinases in Plants[J]. Biotechnology Bulletin, 2013, 0(6): 12-19.

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