基因组编辑技术研究进展

摘要/Abstract

摘要： 利用基因组编辑技术可以对生物基因组特定位点进行人工修饰,研究相关基因的功能,进而应用于基础研究和临床治疗方面。序列特异性的DNA结合结构域与非特异性的DNA修饰结构域组合而成的人工酶是基因组编辑工具的重要组成部分。主要介绍了锌指核酸酶（ZFNs）、转录激活样效应因子核酸酶（TALEN）、归巢核酸内切酶（Meganucleases）和成簇间隔短回文重复（CRISPR）4种基因组编辑技术的特点、原理、构建方法及应用,为相关的研究和应用提供参考。

关键词: 基因编辑, ZFNs, TALEN, CRISPR, 同源重组

Abstract: Genome Editing can be used to operate artificially almost any gene in cells or organisms. This research method of gene function is useful for fundamental research and clinic treatment. The major component of this tool is artificial nuclease with specific DNA-binding domain and non-specific DNA-modifying domain. This article reviews the characteristics, principle, construction and application of ZFN, TALEN and CRISPR/Cas, which would be useful reference for related research.

Key words: Gene editing , ZFNs , TALEN , CRISPR, Homologous recombination

吴璐,王磊,任远,原辉. 基因组编辑技术研究进展[J]. 生物技术通报, 2014, 0(11): 84-90.

Wu Lu ,Wang Lei ,Ren Yuan, Yuan Hui. The Research of Genome Editing[J]. Biotechnology Bulletin, 2014, 0(11): 84-90.

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