重组天花粉蛋白与YH-16联合用药抑制黑色素瘤细胞增殖及成瘤

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.035

生物技术通报 ›› 2016, Vol. 32 ›› Issue (5): 260-264.doi: 10.13560/j.cnki.biotech.bull.1985.2016.05.035

重组天花粉蛋白与YH-16联合用药抑制黑色素瘤细胞增殖及成瘤

郭会灿

石家庄职业技术学院化学工程系，石家庄 050081

收稿日期:2015-06-19 修回日期:2016-05-27 出版日期:2016-05-25 发布日期:2016-05-27
作者简介:郭会灿，女，硕士，副教授，研究方向：生物制药；E-mail：heweibingkkk@126.com

The Inhibition Effect of Recombinant Trichosanthin Combined with YH-16 on the Proliferation and Xenograft Tumor Growth of Melanoma

GUO Hui-can

Department of Chemistry，Shijiazhuang Vocational Technology Institute，Shijiazhuang 050081

Received:2015-06-19 Revised:2016-05-27 Published:2016-05-25 Online:2016-05-27

摘要/Abstract

摘要： 获得人源天花粉蛋白并研究其与YH-16联合用药对小鼠黑色素瘤细胞B16-F10增殖及成瘤的作用。以大肠杆菌BL21重组表达天花粉蛋白rTCS并以亲和层析法纯化rTCS；以MTT法检测rTCS、YH-16、rTCS+YH-16对B16-F10细胞生长的影响；体内实验检测rTCS、rTCS+YH-16对瘤体生长的作用。结果表明，获得了电泳纯重组天花粉蛋白rTCS；rTCS可显著抑制B16-F10细胞增殖，并且抑制率显著高于YH-16组，但是rTCS与YH-16联合处理组并未比rTCS组有显著差异；rTCS与YH-16联合用药明显抑制B16-F10细胞在小鼠体内成瘤。重组天花粉蛋白rTCS联合YH-16用药对黑色素瘤细胞B16-F10的增殖及成瘤有显著抑制作用。联合策略将为治疗黑色素瘤患者提供新的契机。

关键词: 天花粉蛋白, YH-16, B16-F10, 肿瘤, 联合治疗

Abstract: The objectives of this study are to acquire trichosanthin（rTCS）and to explore the effect of rTCS combined with YH-16 on the proliferation of melanoma B16-F10 and xenograft tumor growth in mice model. rTCS was expressed in Escherichia coli BL21 strain and purified by Ni affinity chromatography. Then the effects of rTCS，YH-16，and combination of rTCS with YH-16 on the cell growth were determined by MTT assay and in vivo trial. Results were as：pure recombinant rTCS protein was obtained. The proliferation of B16-F10 was inhibited significantly by rTCS，and inhibition rate was significantly higher than by YH-16 alone，however，no significant difference between rTCS alone and the combination of rTCS and YH-16. The xenograft tumor growth in the group treated with the combination of rTCS and YH-16 was significantly smaller than the ones by rTCS-treated alone or control. In conclusion，the combination of rTCS with YH-16 presented significant inhibitory effect on proliferation of B16-F10 as well as on xenograft tumor growth，and this strategy with the combination would provide new opportunity for treating melanoma patients.

Key words: trichosanthin, YH-16, B16-F10, tumor, combination therapy

郭会灿. 重组天花粉蛋白与YH-16联合用药抑制黑色素瘤细胞增殖及成瘤[J]. 生物技术通报, 2016, 32(5): 260-264.

GUO Hui-can. The Inhibition Effect of Recombinant Trichosanthin Combined with YH-16 on the Proliferation and Xenograft Tumor Growth of Melanoma[J]. Biotechnology Bulletin, 2016, 32(5): 260-264.

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重组天花粉蛋白与YH-16联合用药抑制黑色素瘤细胞增殖及成瘤

The Inhibition Effect of Recombinant Trichosanthin Combined with YH-16 on the Proliferation and Xenograft Tumor Growth of Melanoma

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