生物技术通报 ›› 2016, Vol. 32 ›› Issue (9): 32-37.doi: 10.13560/j.cnki.biotech.bull.1985.2016.09.005

• 综述与专论 • 上一篇    下一篇

二硫键及其连接方式对防御素抗菌功能的影响研究进展

陈惠娴1, 2, 3, 毛若雨1, 3, 滕达1, 3, 王秀敏1, 3, 冯兴军2, 王建华1, 3   

  1. 1. 农业部饲料生物技术重点开放实验室,北京 100081; 2. 东北农业大学动物科技学院,哈尔滨 150030; 3. 中国农业科学院饲料研究因工程室,北京 100081
  • 收稿日期:2016-01-08 出版日期:2016-09-25 发布日期:2016-10-10
  • 作者简介:陈惠娴,女,硕士研究生,研究方向:动物营养与饲料科学;E-mail:1090031362@qq.com
  • 基金资助:
    国家自然科学基金项目(31372346,31302004,31572444,31572445),科技支撑计划项目课题(2013BAD10B02),中国农业科学院农业科技创新工程(CAAS-ASTIP-2013-FRI-02)

Review on Effects of Disulfide Bond and Its Connection on Antimicrobial Activity in Defensins

CHEN Hui-xian, MAO Ruo-yu, TENG Da, WANG Xiu-min, FENG Xing-jun, WANG Jian-hua   

  1. 1. Key Laboratory of Feed Biotechnology,Ministry of Agriculture,Beijing 100081; 2. Institute of Animal Nutrition,Northeast Agricultural University,Harbin 150030; 3. Gene Engineering Laboratory,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2016-01-08 Published:2016-09-25 Online:2016-10-10

摘要: 防御素是一类内源性、高度稳定、富含半胱氨酸的抗菌肽,对抗感染和宿主免疫调控具有重要作用。其序列内一般有6-8个保守半胱氨酸形成3-4对二硫键。二硫键对数和连接方式在维持防御素结构和抗菌功能方面具有重要作用,此外,高活性线型及低二硫键含量突变体在减少生产成本,简化生产流程方面具有重要作用。综述了防御素分子特征、结构和功能,在此基础上报道二硫键氧化还原状态、数量及其连接方式影响防御素抗菌活性的最新研究进展。

关键词: 防御素, 二硫键, 氧化还原, 连接, 抗菌活性

Abstract: Defensins are a class of endogenous,highly stable and cysteine-rich antimicrobial peptides,which play an important role in anti-microbial infection and the regulation of host immune. Six to eight conserved cysteines in the sequence form 3 to 4 pairs of disulfide bonds. The number of disulfide bond and its connection play an crucial role in maintaining the structure and antibacterial functions of defensins. In addition,mutants with high activity and low disulfide bond content also play an key role in reducing production costs and simplifying the production processes. The molecular characteristics,structure and function of defensins and their effects of redox status,number and connection mode of disulfide bonds on the antimicrobial activity are reviewed.

Key words: defensin, disulfide bond, oxidation and reduction, linkage, antimicrobial activity