生物技术通报 ›› 2018, Vol. 34 ›› Issue (5): 48-56.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0891

• 基因编辑专题 • 上一篇    下一篇

非人灵长类动物基因编辑技术的应用及挑战

毕延震1,肖红卫1,张立苹1,任红艳1,华再东1,华文君1,王正1,牛敏杰1,林郑云1,任习东2,孙理华2,郑新民1   

  1. 1. 湖北省农业科学院畜牧兽医研究所 动物胚胎工程及分子育种湖北省重点实验室,武汉 430064;
    2. 湖北天勤生物科技有限公司,武汉 430064
  • 收稿日期:2017-10-23 出版日期:2018-05-26 发布日期:2018-06-07
  • 作者简介:毕延震,男,博士,研究方向:动物生物技术;E-mail:biyanzhen@gmail.com
  • 基金资助:
    转基因生物新品种培育重大专项(2016ZX08010003-006),国家自然科学基金项目(31772577),湖北省农业科技创新中心(2017-620-004-001),湖北省农业科学院青年基金(2016NKYJJ19),湖北省农业科学院竞争性项目(2016jzxjh013),湖北省技术创新专项重大项目(2016ABA117),东南大学生物电子学国家重点实验室开放研究基金,企业合作项目(湖北天勤生物科技有限公司资助)

Applications and Challenges of Gene Editing in Non-human Primate Models

BI Yan-zhen2, XIAO Hong-wei1, ZHANG Li-ping1, REN Hong-yan1, HUA Zai-dong1, HUA Wen-jun1, WANG Zheng1, NIU Min-jie1, LIN Zheng-yun1, REN Xi-dong2, SUN Li-hua2, ZHENG Xin-min1   

  1. 1. Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064;
    2. Hubei Topgene Biotechnology Co.,Ltd.,Wuhan 430064
  • Received:2017-10-23 Published:2018-05-26 Online:2018-06-07

摘要: 建立有效的动物模型是研究人类疾病演进、开发新型治疗手段的重要方法。非人灵长类动物在进化发育、生理生化及病理方面和人类最接近,是研究人类疾病的理想动物模型。随着基因编辑技术的发展,研究者已经成功建立了多种模仿人类疾病的非人灵长类动物模型。但是CRISPR/Cas9的脱靶效应、嵌合突变以及基因敲入效率较低等突出问题也逐渐引起重视。本文综述了基因编辑技术在建立非人灵长类动物模型中的应用现状,提出了目前亟需解决的难点和应对策略,以期为高效、准确构建非人灵长类动物模型提供借鉴与参考。

关键词: 非人灵长类动物模型, TALENs, CRISPR/Cas9, 脱靶效应, 嵌合突变

Abstract: There is a pressing urgency in the medical field to establish an effective animal model to study human diseases. Non-human primates,which are evolutionary,physiologically and pathologically closer to humans than any other animal,are thus considered to be excellent animal models for studies of human diseases. With the development of gene manipulation technologies,researchers have successfully created a variety of non-human primate models via gene editing. Yet,there are still some challenges we have to face,such as off-target,mosaic mutants,and low efficiency of gene knock-in in CRISPR/Cas9. In this paper,we summarize the applications of gene editing in non-human primate models,as well as the existing problems and improvements,aimed to provide a reference for further research in non-human primate models mimicking human diseases.

Key words: non-human primate models, TALENs, CRISPR/Cas9, off-target, mosaic mutants