生物技术通报 ›› 2019, Vol. 35 ›› Issue (10): 71-79.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0303

• 研究报告 • 上一篇    下一篇

外源硫与乙烯缓解马齿苋镉胁迫的生理机制研究

王竹承1, 刘辉2, 李荣华1, 陈新1, 李欣1, 路致远1   

  1. 1. 沧州师范学院生命科学学院,沧州 061001;
    2. 贵州理工学院食品药品制造工程学院,贵阳 550003
  • 收稿日期:2019-04-11 出版日期:2019-10-26 发布日期:2019-09-30
  • 作者简介:王竹承,男,硕士,研究方向:植物逆境生理;E-mail:1604089148@qq.com
  • 基金资助:
    沧州市科技局课题(183203002)

Physiological Mechanism of Exogenous Ethylene and Sulfur in Alleviating Cadmium Stress in Portulaca oleracea

WANG Zhu-cheng1, LIU Hui2, LI Rong-hua1, CHEN Xin1, LI Xin1, LU Zhi-yuan1   

  1. 1. College of Life Science,Cangzhou Normal University,Cangzhou 061001;
    2. College of Food and Pharmaceutical Engineering,Guizhou Institute of Technology,Guiyang 550003
  • Received:2019-04-11 Published:2019-10-26 Online:2019-09-30

摘要: 解析镉(Cd)胁迫下外源乙烯和硫(S)对马齿苋(Portulaca oleracea L.)生理响应的机制。在Cd胁迫下,检测乙烯利 (乙烯供体)和(NH42SO4对马齿苋叶片氧化胁迫、S同化吸收、葡萄糖含量、乙烯合成、光合作用的影响。结果显示,外源S和乙烯处理,可降低Cd胁迫下马齿苋叶片与根中的Cd含量;增强ATP硫酸化酶(ATP-S)和谷胱甘肽还原酶(GR)活性,提升马齿苋叶片还原型谷胱甘肽(GSH)含量,从而降低叶片H2O2与丙二醛(MDA)含量;增强1-氨基环丙烷羧酸合酶(ACS)活性,提升叶片乙烯含量,从而降低葡萄糖含量,提升Rubisco酶活性和光合作用。同时添加外源S和乙烯,对上述指标的促进或者抑制效果加强,而添加乙烯活性专一性抑制剂二环庚二烯(Norbornadiene,NBD)则对上述各指标具有反向作用。外源S和乙烯可以通过降低马齿苋对Cd的吸收,增强体内乙烯信号传导途径,促进GSH的合成,降低葡萄糖含量,从而缓解Cd胁迫诱导的氧化胁迫和葡萄糖介导的光合抑制作用。

关键词: 镉胁迫, 乙烯, 硫, 马齿苋, 生理特性

Abstract: To uncover the physiological mechanism of exogenous ethylene and sulfur(S)in response to cadmium(Cd)stress,the effects of ethephon(ethylene donor)on the oxidative stress,S assimilation,glucose content,ethylene biosynthesis,and photosynthesis in leaf of P. oleracea were detected. The results revealed that exogenous ethylene and S-treatment decreased the Cd content in the leave and root of P. oleracea under Cd stress,also decreased H2O2accumulation and malondialdehyde(MDA)content via enhancing the activities of ATP sulfase(ATP-S)and glutathione reductase(GR)as well as increasing the content of reduced glutathion(GSH)in P. oleracea leaves. Moreover,the application of S or ethylene resulted in the glucose content reducing and Rubisco enzyme activity and photosynthesis increasing via increasing the activity of 1-aminocyclopropane carboxylic acid enzyme(ACS)and ethylene content in leaves. Simultaneous addition of exogenous ethylene and S enhanced above promoting and inhibitory effects,but the application of ethylene action inhibitor norbornadiene(NBD)resulted in the reverse effects of above indexes. The results suggest that exogenous S and ethylene alleviates the Cd- induced oxidative stress and glucose-mediated photosynthesis inhibition in P. oleracea through decreasing Cd absorption,enhancing internal ethylene signaling pathways,promoting GSH production,and decreasing glucose content.

Key words: cadmium stress, ethylene, sulfur, Portulaca oleracea L., physiological characteristic