大肠杆菌代谢途径改造策略与应用研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-0131

摘要/Abstract

摘要： 大肠杆菌由于具有稳定性强和易于操作的特点成为基因改造常用的宿主微生物。利用基因工程手段改造大肠杆菌的代谢途径,可用于生物燃料、手性药物及其衍生物的合成。利用代谢组学和合成生物学能够高效地以大肠杆菌作为生物催化剂生产目标物。综述了大肠杆菌中丙酮酸、乙酰辅酶A、甲羟戊酸和莽草酸代谢途径的改造策略,以及大肠杆菌代谢途径的改造在合成生物燃料、砌块化合物中的应用,为研究者以大肠杆菌合成目标化合物的研究提供一个整体的思路和方法。

关键词: 大肠杆菌, 代谢途径, 生物燃料, 砌块化合物

Abstract: The metabolic pathway of Escherichia coli can be modified by genetic engineering,which can be used for the synthesis of biofuels,chiral drugs,and their derivatives. E. coli has become a common host microorganism for genetic modification due to its strong stability and easy operation. Using metabolomics and synthetic biology,E. coli can be efficiently used as a target for biocatalytic production. This paper reviews the strategies of metabolic pathway modification of pyruvate,acetyl coenzyme A,mevalonate and shikimic acid in E. coli,and the application of metabolic pathway modification of E. coli in the synthesis of biofuels and block compounds,which provides a holistic idea and method for researchers to study the synthesis of target compounds by E. coli.

Key words: Escherichia coli, metabolic pathway, biofuel, block compounds

李冉, 黄玉清, 贾振华. 大肠杆菌代谢途径改造策略与应用研究进展[J]. 生物技术通报, 2019, 35(8): 232-237.

LI Ran, HUANG Yu-qing, JIA Zhen-hua. Research of Progress of Strategy and Application of Metabolic Pathway Modification in Escherichia coli[J]. Biotechnology Bulletin, 2019, 35(8): 232-237.

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