生物技术通报 ›› 2021, Vol. 37 ›› Issue (11): 166-177.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1110
• 食用菌生物技术专题(专题主编: 黄晨阳) • 上一篇 下一篇
谭昊1,2(), 刘天海1, 闫世杰1, 余洋1, 姜邻1, 彭卫红1
收稿日期:
2021-08-29
出版日期:
2021-11-26
发布日期:
2021-12-03
作者简介:
谭昊,男,博士,副研究员,研究方向:环境微生物生理生态机制;E-mail: 基金资助:
TAN Hao1,2(), LIU Tian-hai1, YAN Shi-jie1, YU Yang1, JIANG Lin1, PENG Wei-hong1
Received:
2021-08-29
Published:
2021-11-26
Online:
2021-12-03
摘要:
为开发沙漠砂代替土壤作为羊肚菌栽培基质,研究羊肚菌栽培对沙漠砂中微生物群落及基质理化性质的影响。利用扩增子测序分别检测栽培与未栽培羊肚菌的沙漠砂中细菌、真菌群落,解析总有机碳、腐殖质和氮素含量等理化性质。收获子实体产量(1 210±259)g/m2鲜重,说明沙漠砂栽培羊肚菌可行。沙漠砂基质中微生物多样性在羊肚菌栽培过程中短暂下降,在羊肚菌出菇采收后回升。真菌群落以子囊菌门为主。随着羊肚菌在沙漠砂中增殖,扫描电镜观察到大量菌丝形成网络状缠绕固定沙漠砂颗粒。细菌群落以变形菌门、放线菌门、拟杆菌门为主。假单胞菌属相对丰度在羊肚菌菌丝生长期明显上升,蓝细菌门在羊肚菌采收后大幅度扩增,伴随总氮和铵态氮含量显著上升,超越了栽培羊肚菌之前的初始水平。栽培羊肚菌使沙漠砂中有机质含量提升7倍,出菇后基质中胡敏酸、富啡酸、胡敏素含量显著上升。研究结果揭示了用沙漠砂种植羊肚菌在收获高值农产品的同时改良沙漠砂理化性质的应用潜力。
谭昊, 刘天海, 闫世杰, 余洋, 姜邻, 彭卫红. 羊肚菌栽培对沙漠砂基质中微生物群落及基质理化性质的影响[J]. 生物技术通报, 2021, 37(11): 166-177.
TAN Hao, LIU Tian-hai, YAN Shi-jie, YU Yang, JIANG Lin, PENG Wei-hong. Impacts of Morel Cultivation on the Microbial Community and Physiochemical Characteristics in a Substratum of Desert Sand[J]. Biotechnology Bulletin, 2021, 37(11): 166-177.
图1 沙漠砂基质中细菌群落(A)与真菌群落(B)Shannon-Wiener多样性指数的变化
Fig. 1 Shift of Shannon-Wiener diversity index in the bacterial(A)and fungal(B)communities in the desert-sand substratum during morel cultivation
图3 微生物群落组成 A:细菌门;B:细菌属;C:真菌门;D:真菌属
Fig. 3 Taxonmic composition of the microbial communities A: Bacterial phylum;B: bacterial genus;C: fungal phylum;D: fungal genus
图4 微生物群落OTU组成差异 A:栽培与未栽培羊肚菌;B:不同时间点
Fig. 4 Differences in the OTU composition among the microbial communities A: With or without morel cultivation;B: Different time-points
图5 LEfSe分析:栽培与不栽培羊肚菌的沙漠砂基质中细菌群落(A)与真菌群落(B)的标志性类群
Fig. 5 LEfSe analysis:Biomarker taxa of the bacterial communities(A)and fungal communities(B)in the desert-sand substratum cultivated with morel or not
[1] |
Liu Q, Ma H, Zhang Y, et al. Artificial cultivation of true morels:current state, issues and perspectives[J]. Crit Rev Biotechnol, 2018, 38(2): 259-271.
doi: 10.1080/07388551.2017.1333082 URL |
[2] | 赵洁, 焦沛尧. 羊肚菌种植产业对贫困地区经济发展的促进作用研究——基于河南工业大学材料学院“菌准扶贫”实践团队的实践与调研[J]. 乡村科技, 2018(29): 28-29. |
Zhao J, Jiao PY. Research on the promotional effect of morel mushroom planting industry on economic development in poverty-stricken areas——Based on the practice and investigation of the poverty alleviation practice team of the School of Materials Science and Engineering of Henan University of Technology[J]. Xiang Cun Ke Ji, 2018(29): 28-29. | |
[3] |
Ower R. Notes on the development of the morel ascocarp:Morchella esculenta[J]. Mycologia, 1982, 74(1): 142-144.
doi: 10.1080/00275514.1982.12021480 URL |
[4] | 贺新生. 羊肚菌生物学基础、菌种分离制作与高产栽培技术[M]. 北京: 科学出版社, 2021. |
He XS. The biological basis, strain isolation and production and high-yield cultivation techniques of Morchella esculenta[M]. Beijing: Science Press, 2021. | |
[5] | 刘伟, 张亚, 何培新. 羊肚菌生物学与栽培技术[M]. 长春: 吉林科学技术出版社, 2017. |
Liu W, Zhang Y, He PX. Morel biology and cultivation[M]. Changchun: Jilin Science & Technology Publishing House, 2017. | |
[6] | 何培新, 楼海军, 申进文. 郑州市粗柄羊肚菌生境调查分析[J]. 河南农业科学, 2009, 38(2): 95-97. |
He PX, Lou HJ, Shen JW. The habitat investigation and analysis of morel grits in Zhengzhou city[J]. J Henan Agric Sci, 2009, 38(2): 95-97. | |
[7] | 四川省质量技术监督局. 中华人民共和国地震行业标准:羊肚菌大田栽培技术规程 DB51/T 2094—2015[S]. 四川省农业科学院土壤肥料研究所:四川省质量技术监督局, 2015. |
Sichuan Provincial Bureau of Quality and Technical Supervision. Earthquake Standard of the People’s Republic of China:Technical Regulations for Field Cultivation of Morel DB51/T 2094—2015[S]. Institute of Soil and Fertilizer, Sichuan Academy of Agricultural Sciences:Sichuan Provincial Bureau of Quality and Technical Supervision, 2015. | |
[8] | 甘炳成, 唐杰, 吴翔, 等. 一种羊肚菌增产剂及其应用:CN104945195B[P]. 2018-03-27. |
Gan BC, Tang J, Wu X, et al. Morchella esculenta yield increase agent and application thereof:CN104945195B[P]. 2018-03-27. | |
[9] | 肖千明, 李红, 刘娜, 等. 一种促进羊肚菌生长的微生物海藻液肥及其应用:CN108484314A[P]. 2018-09-04. |
Xiao QM, Li H, Liu N, et al. Microorganism seaweed liquid fertilizer for promoting growth of toadstool and application of microorganism seaweed liquid fertilizer:CN108484314A[P]. 2018-09-04. | |
[10] |
Tan H, Kohler A, Miao RY, et al. Multi-omic analyses of exogenous nutrient bag decomposition by the black morel Morchella importuna reveal sustained carbon acquisition and transferring[J]. Environ Microbiol, 2019, 21(10): 3909-3926.
doi: 10.1111/emi.v21.10 URL |
[11] |
Tan H, Yu Y, Tang J, et al. Build your own mushroom soil:microbiota succession and nutritional accumulation in semi-synthetic substratum drive the fructification of a soil-saprotrophic morel[J]. Front Microbiol, 2021, 12: 656656. DOI: 10.3389/fmicb.2021.656656.
doi: 10.3389/fmicb.2021.656656 URL |
[12] | 陈新闯, 郭建英, 董智, 等. 乌兰布和沙漠乌海段新月形沙丘表层沉积物粒度与重金属分布特征[J]. 水土保持学报, 2015, 29(3): 47-51. |
Chen XC, Guo JY, Dong Z, et al. Characteristics of heavy metal distribution and grain size in surface sediments of barchan dunes of the Wuhai section in μLan buh desert[J]. J Soil Water Conserv, 2015, 29(3): 47-51. | |
[13] |
Xie GH, Steinberger Y. Nitrogen and carbon dynamics under the canopy of sand dune shrubs in a desert ecosystem[J]. Arid Land Res Manag, 2005, 19(2): 147-160.
doi: 10.1080/15324980590916549 URL |
[14] | 张玉兰, 孙彩霞, 段争虎, 等. 光谱法分析固沙工程对土壤腐殖质及组分的影响[J]. 光谱学与光谱分析, 2010, 30(1): 179-183. |
Zhang YL, Sun CX, Duan ZH, et al. Effect of sand-stabilization engineering on soil humus and components by analysis by several spectroscopy methods[J]. Spectrosc Spectr Anal, 2010, 30(1): 179-183. | |
[15] |
Fattahi SM, Soroush A, Huang N, et al. Laboratory study on biophysicochemical improvement of desert sand[J]. CATENA, 2020, 190: 104531.
doi: 10.1016/j.catena.2020.104531 URL |
[16] | 段宏美, 窦森, 王德辉, 等. 玉米浆及配合其他有机物料对苏打碱土改良效果及腐殖质特性变化研究[J]. 农业环境科学学报, 2021, 40(10): 2159-2166. |
Duan HM, Dou S, Wang DH, et al. Study on the improvement effect of corn steep liquor and other organic materials on alkaline soil and the change in humus characteristics[J]. Journal of Agro-Environment Science, 2021, 40(10): 2159-2166. | |
[17] |
Rodriguez-Caballero E, Belnap J, Büdel B, et al. Dryland photoautotrophic soil surface communities endangered byglobal change[J]. Nat Geosci, 2018, 11(3): 185-189.
doi: 10.1038/s41561-018-0072-1 URL |
[18] | Fischer T, Yair A, Veste M. Microstructure and hydraulic properties of biological soil crusts on sand dunes:a comparison between arid and temperate climates[J]. Biogeosciences Discuss, 2012, 9: 12711-12734. |
[19] | 苗人云, 刘天海, 罗建华, 等. 羊肚菌营养袋制作原料的化学成分分析及配方优化[J]. 食药用菌, 2020, 28(2): 112-118. |
Miao RY, Liu TH, Luo JH, et al. Chemical composition analysis and formula optimization of exogenous nutrient bag for morel cultivation[J]. Edible Med Mushrooms, 2020, 28(2): 112-118. | |
[20] | 谭昊, 苗人云, 刘天海, 等. 成都平原地区梯棱羊肚菌人工栽培生态环境参数范围及变化规律[J]. 西南农业学报, 2021, 34(1): 27-39. |
Tan H, Miao RY, Liu TH, et al. Ranges and dynamics of environmental factors for Morchella importuna cultivation in Chengdu plain area[J]. Southwest China J Agric Sci, 2021, 34(1): 27-39. | |
[21] |
Carrigg C, Rice O, Kavanagh S, et al. DNA extraction method affects microbial community profiles from soils and sediment[J]. Appl Microbiol Biotechnol, 2007, 77(4): 955-964.
pmid: 17960375 |
[22] |
Jiang YJ, Li SZ, Li RP, et al. Plant cultivars imprint the rhizosphere bacterial community composition and association networks[J]. Soil Biol Biochem, 2017, 109: 145-155.
doi: 10.1016/j.soilbio.2017.02.010 URL |
[23] |
French KE, Tkacz A, Turnbull LA. Conversion of grassland to arable decreases microbial diversity and alters community composition[J]. Appl Soil Ecol, 2017, 110: 43-52.
doi: 10.1016/j.apsoil.2016.10.015 URL |
[24] |
Aye NS, Sale PWG, Tang CX. The impact of long-term liming on soil organic carbon and aggregate stability in low-input acid soils[J]. Biol Fertil Soils, 2016, 52(5): 697-709.
doi: 10.1007/s00374-016-1111-y URL |
[25] | Jackson ML. Soil Chemical Analysis[M]. New Delhi:Prentice Hall Inc., 1973. |
[26] |
Tian Q, Chen F, Liu J, et al. Inhibition of maize root growth by high nitrate supply is correlated with reduced IAA levels in roots[J]. J Plant Physiol, 2008, 165(9): 942-951.
doi: 10.1016/j.jplph.2007.02.011 URL |
[27] |
Tabatabai MA, Bremner JM. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity[J]. Soil Biol Biochem, 1969, 1(4): 301-307.
doi: 10.1016/0038-0717(69)90012-1 URL |
[28] | Ward FN, Nakagawa HM, Harms TF, et al. Atomic-absorption methods of analysis useful in geochemical exploration[R]. US Geological Survey, 1969. |
[29] |
Behera SK, Shukla AK, Suresh K, et al. Oil palm cultivation enhances soil pH, electrical conductivity, concentrations of exchangeable calcium, magnesium, and available sulfur and soil organic carbon content[J]. Land Degrad Dev, 2020, 31(18): 2789-2803.
doi: 10.1002/ldr.v31.18 URL |
[30] |
Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nat Methods, 2010, 7(5): 335-336.
doi: 10.1038/nmeth.f.303 pmid: 20383131 |
[31] |
Edgar RC. UPARSE:highly accurate OTU sequences from microbial amplicon reads[J]. Nat Methods, 2013, 10(10): 996-998.
doi: 10.1038/nmeth.2604 URL |
[32] |
Gdanetz K, Benucci GMN, Vande Pol N, et al. CONSTAX:a tool for improved taxonomic resolution of environmental fungal ITS sequences[J]. BMC Bioinformatics, 2017, 18(1): 538.
doi: 10.1186/s12859-017-1952-x pmid: 29212440 |
[33] | Oksanen J, Blanchet FG, Kindt R, et al. Vegan:community ecology package. R package version 2. 4-4[EB/OL]. https://CRAN.R-project.org/package=vegan, 2016. |
[34] | Ginestet C. ggplot2:elegant graphics for data analysis[J]. J Royal Stat Soc:Ser A Stat Soc, 2011, 174(1): 245-246. |
[35] |
Segata N, Izard J, Waldron L, et al. Metagenomic biomarker discovery and explanation[J]. Genome Biol, 2011, 12(6): R60.
doi: 10.1186/gb-2011-12-6-r60 URL |
[36] | 彭卫红, 唐杰, 何晓兰, 等. 四川羊肚菌人工栽培的现状分析[J]. 食药用菌, 2016, 24(3): 145-150. |
Peng WH, Tang J, He XL, et al. Analysis on the status quo of artificial cultivation of morels in Sichuan[J]. Edible Med Mushrooms, 2016, 24(3): 145-150. | |
[37] |
Benucci GMN, Longley R, Zhang P, et al. Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses[J]. PeerJ, 2019, 7: e7744.
doi: 10.7717/peerj.7744 URL |
[38] | 易志坚. 沙漠“土壤化”生态恢复理论与实践[J]. 重庆交通大学学报:自然科学版, 2016, 35(S1): 27-32. |
Yi ZJ. Theory and practice of desert “soilization”for ecological restoration[J]. J Chongqing Jiaotong Univ:Nat Sci, 2016, 35(S1): 27-32. | |
[39] |
武杞蔓, 张金梅, 李玥莹, 等. 有益微生物菌肥对农作物的作用机制研究进展[J]. 生物技术通报, 2021, 37(5): 221-230.
doi: 10.13560/j.cnki.biotech.bull.1985.2020-0846 |
Wu QM, Zhang JM, Li YY, et al. Recent advances on the mechanism of beneficial microbial fertilizers in crops[J]. Biotechnol Bull, 2021, 37(5): 221-230. | |
[40] | 汪晓文, 李明, 胡云龙. 高质量发展背景下戈壁农业发展的推进路径——来自以色列沙漠农业实践的启示[J]. 开发研究, 2020(3): 48-52. |
Wang XW, Li M, Hu YL. On operating path of Gobi agriculture under the background of high quality development:enlightenment of practice of desert agriculture in Israel[J]. Res Dev, 2020(3): 48-52. | |
[41] | 张林平. 沙特埃及沙漠干旱地区农业及生态建设的主要经验[J]. 农业环境与发展, 2002, 19(4): 39-41. |
Zhang LP. The main experience of agriculture and ecological construction in arid desert areas in Saudi Arabia and Egypt[J]. Agro Environ Dev, 2002, 19(4): 39-41. |
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