生物技术通报 ›› 2022, Vol. 38 ›› Issue (6): 129-135.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0963

• 研究报告 • 上一篇    下一篇

GRAS转录因子AtSCL4负调控拟南芥应答渗透胁迫

徐红云(), 张明意   

  1. 贵州民族大学生态环境工程学院,贵阳 550025
  • 收稿日期:2021-07-28 出版日期:2022-06-26 发布日期:2022-07-11
  • 基金资助:
    贵州省教育厅青年科技人才成长项目(黔教合KY字[2021]106)

AtSCL4,an Arabidopsis thaliana GRAS Transcription Factor,Negatively Modulates Plants in Response to Osmotic Stress

XU Hong-yun(), ZHANG Ming-yi   

  1. College of Eco-environment Engineering,Guizhou Minzu University,Guiyang 550025
  • Received:2021-07-28 Published:2022-06-26 Online:2022-07-11

摘要:

探讨拟南芥GRAS转录因子AtSCL4(Arabidopsis thaliana SCL4)在渗透胁迫中发挥的生物学功能,为GRAS蛋白在非生物胁迫中的功能研究奠定基础。以野生型和AtSCL4突变体拟南芥为试验材料,通过生理指标测定和qRT-PCR方法研究渗透胁迫下AtSCL4调控植物抗逆的生物学机制。研究发现AtSCL4受渗透胁迫诱导后显著上调表达,且AtSCL4突变体的抗渗能力强于野生型。在渗透胁迫下,AtSCL4可以负调节ATMYB6的表达,减小气孔开放度,降低叶片水分流失;AtSCL4通过负调控P5SC1和BADH的转录来提高植物体内脯氨酸和甜菜碱的含量;AtSCL4通过负调节AtSOD1和PER4的表达来增加抗氧化酶活性而降低活性氧含量。AtSCL4可负向调控抗逆基因表达和生理变化应答渗透胁迫。

关键词: 拟南芥, 渗透胁迫, GRAS转录因子, 气孔, 活性氧

Abstract:

Exploring the biological function of Arabidopsis thaliana GRAS transcription factor AtSCL4 in response to osmotic stress would lay foundation for studying the function of GRAS protein in abiotic stresses. Wild-type and AtSCL4 mutant were used as experimental materials,and physiological index determination and qRT-PCR methods were applied to study the biological mechanism of AtSCL4 regulating plant osmosis resistance. The study revealed that AtSCL4 was significantly up-regulated by osmotic stress,and mutants of AtSCL4 showed improved osmotic resistance than WT. In response to osmotic stress,AtSCL4 negatively regulated AtMYB61 expression to increase stomatal closure,resulting in reducing water loss. Furthermore,AtSCL4 improved proline and betaine levels by regulating the transcripts of P5SC1 and BADH. In addition,AtSCL4 increased antioxidant enzyme activity by negatively regulating AtSOD1 and PER4 expressions,leading to the decrease of reactive oxygen. AtSCL4 negatively modulated osmotic stress tolerance via the induction of stress-responsive genes and physiological changes.

Key words: Arabidopsis thaliana, osmotic stress, GRAS transcription factor, stomatal aperture, ROS