生物技术通报 ›› 2023, Vol. 39 ›› Issue (2): 274-282.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0826

• 研究报告 • 上一篇    下一篇

大肠杆菌型奶牛乳房炎对产奶性状相关基因表达的影响

李彦霞1,2(), 王晋鹏1,2, 冯芬1,2, 包斌武1,2, 董益闻1,2, 王兴平1,2, 罗仍卓么1,2()   

  1. 1.宁夏大学农学院,银川 750021
    2.宁夏回族自治区反刍动物分子细胞育种重点实验室,银川 750021
  • 收稿日期:2022-07-04 出版日期:2023-02-26 发布日期:2023-03-07
  • 作者简介:李彦霞,女,硕士,研究方向:动物分子遗传育种;E-mail: lyx25252500@163.com
  • 基金资助:
    国家自然科学基金地区项目(32060749);宁夏自然科学基金项目(2022AAC03056);宁夏高等学校科学研究(优秀青年教师培育基金项目)项目(NGY2020007)

Effects of Escherichia coli Dairy Cow Mastitis on the Expressions of Milk-producing Trait Related Genes

LI Yan-xia1,2(), WANG Jin-peng1,2, FENG Fen1,2, BAO Bin-wu1,2, DONG Yi-wen1,2, WANG Xing-ping1,2, LUORENG Zhuo-ma1,2()   

  1. 1. School of Agricultural, Ningxia University, Yinchuan 750021
    2. Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan 750021
  • Received:2022-07-04 Published:2023-02-26 Online:2023-03-07

摘要:

旨为从基因水平上探究大肠杆菌(Escherichia coli)型乳房炎奶牛的产奶量和乳品质下降的原因。本实验利用脂多糖(LPS)诱导乳腺上皮细胞(bMECs)建立细胞炎症模型,并采用实时荧光定量PCR技术检测了奶牛产奶量与乳蛋白相关基因(CSN1S1CSN2LALBA)、乳脂调控相关基因(FABP3LPL)以及炎症与产奶量相关基因MFGE8在奶牛乳腺组织和bMECs炎症反应中的表达量。结果表明,与对照组(0 h)相比,上述6个基因在E.coli型乳房炎奶牛的乳腺组织中表达量均极显著下调(P < 0.01)。此外,上述6个基因在LPS诱导bMECs产生炎症反应的3、6、12和24 h的bMECs中表达量也均极显著下调(P < 0.01)。在E.coli型奶牛乳房炎发生或bMECs产生炎症后,上述6个基因的表达水平均受到抑制,解释了E.coli型乳房炎导致产奶量减少、乳蛋白率和乳脂率下降的原因。

关键词: Escherichia coli, 乳房炎, 产奶性状, 乳腺上皮细胞, 基因表达

Abstract:

This work aims to explore the reasons of milk yield and milk quality declining from dairy cows with Escherichia coli mastitis at the genetic level. This experiment established a cell inflammation model by lipopolysaccharide(LPS)-induced bovine mammary epithelial cells(bMECs). The expressions of milk yield and milk protein-related genes(CSN1S1, CSN2 and LALBA), milk fat regulation-related genes(FABP3 and LPL)and inflammation and milk yield-related gene MFGE8 were detected by real-time quantitative PCR(qPCR). The results showed that the expressions of the above 6 genes were significantly down-regulated in the mammary tissues of dairy cows with E.coli mastitis(P < 0.01)compared with the control group(0 h). In addition, the expressions of the six genes were also significantly down-regulated in LPS-induced bMECs at 3, 6, 12 and 24 h(P < 0.01). This indicated that the expressions of the six genes were inhibited after E. coli mastitis or bMECs inflammation occurred, which explained the reasons for E.coli mastitis reducing milk yield, milk protein percentage and milk fat percentage.

Key words: Escherichia coli, mastitis, milk-producing trait, mammary epithelial cells, gene expression