最短尾短体线虫转录组及潜在效应蛋白分析

doi:10.13560/j.cnki.biotech.bull.1985.2022-0773

生物技术通报 ›› 2023, Vol. 39 ›› Issue (3): 254-266.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0773

最短尾短体线虫转录组及潜在效应蛋白分析

扈丽丽¹^,²(), 林柏荣²^,³, 王宏洪⁴, 陈建松²^,³, 廖金铃⁴(), 卓侃²^,³()

1.广东省森林培育与保护利用重点实验室广东省林业科学研究院，广州 510520
2.华南农业大学植物线虫研究室，广州 510642
3.广东省微生物信号与病害防治重点实验室，广州 510642
4.广东生态工程职业学院，广州 510520

收稿日期:2022-06-25 出版日期:2023-03-26 发布日期:2023-04-10
通讯作者: 廖金铃，男，博士，教授，研究方向：植物病原线虫学；E-mail: jlliao@scau.edu.cn；
卓侃，男，博士，教授，研究方向：植物病原线虫学；E-mail: zhuokan@scau.edu.cn
作者简介:扈丽丽，女，博士，副研究员，研究方向：植物病原线虫学及林业有害生物综合防控；E-mail: hulili0113@163.com
基金资助:
国家自然科学基金项目(31401716);国家自然科学基金项目(31772136);国家自然科学基金项目(31672007)

Transcriptome and Candidate Effectors Analysis of Pratylenchus brachyurus

HU Li-li¹^,²(), LIN Bo-rong²^,³, WANG Hong-hong⁴, CHEN Jian-song²^,³, LIAO Jin-ling⁴(), ZHUO Kan²^,³()

1. Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520
2. Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642
3. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642
4. Guangdong Eco-Engineering Polytechnic, Guangzhou 510520

Received:2022-06-25 Published:2023-03-26 Online:2023-04-10

摘要/Abstract

摘要：

最短尾短体线虫（Pratylenchus brachyurus）是一种重要的迁移性内寄生植物病原线虫。对其转录组进行测序和分析，是进一步研究其寄生特点和致病机理的重要基础。通过转录组测序和分析，得到72 516个unigenes，其中38 266个（52.76%）unigenes注释到7个蛋白质数据库中。Cazyme分析预测到 541 条具有植物/真菌细胞壁降解活性的蛋白质序列，分别属于 GH5、GH30、GH53、GH28、PL3、GH16、GH18和GH20家族。转录组中有56个蛋白序列与秀丽隐杆线虫（Caenorhabditis elegans）的RNAi通路蛋白具有同源性。鉴定到51个与已知植物寄生线虫效应蛋白具有同源性的蛋白序列，检测了9个潜在效应蛋白在抑制植物防卫反应中的作用，其中7个能够抑制由Gpa2/Rbp-1引起的细胞程序性死亡反应。研究结果表明最短尾短体线虫转录组与同属的短体线虫基因分布特点相似，其潜在效应蛋白具有抑制植物防卫反应的特点。

关键词: 最短尾短体线虫, 转录组, 效应蛋白, 细胞程序性死亡

Abstract:

Pratylenchus brachyurus is an important migratory endoparasitic phytopathogenic nematode. Analysis of the transcriptome is an important basis for further research on its parasitic characteristics and pathogenic mechanism. In the transcriptome of P. brachyurus, 72 516 unigenes were generated and 38 266（52.76%）unigenes were annotated against seven protein databases. In the carbohydrate-active enzymes analysis, 541 proteins were predicted to have plant/fungal cell wall degrading activities, and belonged to the families of GH5, GH30, GH53, GH28, PL3, GH16, GH18 and GH20 respectively. There were 56 protein sequences in the transcriptome demonstrating similarities with orthologues involved in RNAi pathway of Caenorhabditis elegans. Further analysis showed that 51 protein sequences with homology to known plant-parasitic nematode effector proteins were identified. Nine candidate effectors were selected and their potential ability in suppressing plant defense was tested. Seven of them suppressed programmed cell death（PCD）triggered by Gpa2/Rbp-1. The results revealed that the gene distribution characteristics of P. brachyurus were similar to of the same genus of Pratylenchus spp. The candidate effectors may play a critical role in suppressing plant defense.

Key words: Pratylenchus brachyurus, transcriptome, effector, programmed cell death

扈丽丽, 林柏荣, 王宏洪, 陈建松, 廖金铃, 卓侃. 最短尾短体线虫转录组及潜在效应蛋白分析[J]. 生物技术通报, 2023, 39(3): 254-266.

HU Li-li, LIN Bo-rong, WANG Hong-hong, CHEN Jian-song, LIAO Jin-ling, ZHUO Kan. Transcriptome and Candidate Effectors Analysis of Pratylenchus brachyurus[J]. Biotechnology Bulletin, 2023, 39(3): 254-266.

图/表 12

参考文献 45

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Primer name	Primer sequence（5'-3'）	Application
45363F	ATGCTGTTCACCGCACTGCT	ORF amplification
45363R	TTATGTTCTCTTCACCTCGC	ORF amplification
39081F	ATGGTCTTCAAAAGCGCTTC	ORF amplification
39081R	CTAATGCTGTTTCATCATTC	ORF amplification
38325F	ATGCGTGCCAATTTGTTGTG	ORF amplification
38325R	TCAATTGAGGCAATCACGCT	ORF amplification
40687F	ATGCATCTAGTTCTATTACT	ORF amplification
40687R	TCAGTCACGTTTGGCCAACT	ORF amplification
41828F	ATGTTCCGCTGCTCTTCCTC	ORF amplification
41828R	TCATTCCTCCTCTCGGTTCT	ORF amplification
49596F	ATGGGCATTGGCTTGGCCAT	ORF amplification
49596R	TCAGACCAACGGGTACGACG	ORF amplification
50445F	ATGGCTACATCAATTTTTGT	ORF amplification
50445R	TCAAAAAGCGATGAATGCGA	ORF amplification
51705F	ATGCTTTCTTGCCGTCTCCTT	ORF amplification
51705R	TCAAATGGGATGATTCAACA	ORF amplification
41042F	ATGTTCTTTACTGTTGCTGG	ORF amplification
41042R	TCAAATTCGAAAGCGTTCAA	ORF amplification
828-1305F	TGACCATGGAAATTCCTGCTCGACAATTTGG	Vector construction
828-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCTTCCTCCTCTCGGTTCTCTC	Vector construction
363-1305F	TGACCATGGAACAACAGGAAAAATATCAGCC	Vector construction
363-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCTGTTCTCTTCACCTCGCCCT	Vector construction
081-1305F	TGACCATGGAA ATCGTCAGTGCGCTTCCCCG	Vector construction
081-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCATGCTGTTTCATCATTCCTG	Vector construction
325-1305F	TGACCATGGAA ATTGGCATTGGCCGAACGCA	Vector construction
325-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCATTGAGGCAATCACGCTCCT	Vector construction
687-1305F	TGACCATGGAA GCCAGCTGTGATTCGCCAAA	Vector construction
687-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCGTCACGTTTGGCCAACTGTT	Vector construction
596-1305F	TGACCATGGAA GTGCAATGGCGTGTTGAGCG	Vector construction
596-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCGACCAACGGGTACGACGCCT	Vector construction
445-1305F	TGACCATGGAA CAATCTCCCAATGCAGATAA	Vector construction
445-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCAAAAGCGATGAATGCGACAG	Vector construction
705-1305F	TGACCATGGAA GAACAATACGATTCAACGGA	Vector construction
705-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCAATGGGATGATTCAACAGGA	Vector construction
042-1305F	TGACCATGGAA CTGGGCTGTATGAGCACAAT	Vector construction
042-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCAATTCGAAAGCGTTCAATCA	Vector construction

Primer name	Primer sequence（5'-3'）	Application
45363F	ATGCTGTTCACCGCACTGCT	ORF amplification
45363R	TTATGTTCTCTTCACCTCGC	ORF amplification
39081F	ATGGTCTTCAAAAGCGCTTC	ORF amplification
39081R	CTAATGCTGTTTCATCATTC	ORF amplification
38325F	ATGCGTGCCAATTTGTTGTG	ORF amplification
38325R	TCAATTGAGGCAATCACGCT	ORF amplification
40687F	ATGCATCTAGTTCTATTACT	ORF amplification
40687R	TCAGTCACGTTTGGCCAACT	ORF amplification
41828F	ATGTTCCGCTGCTCTTCCTC	ORF amplification
41828R	TCATTCCTCCTCTCGGTTCT	ORF amplification
49596F	ATGGGCATTGGCTTGGCCAT	ORF amplification
49596R	TCAGACCAACGGGTACGACG	ORF amplification
50445F	ATGGCTACATCAATTTTTGT	ORF amplification
50445R	TCAAAAAGCGATGAATGCGA	ORF amplification
51705F	ATGCTTTCTTGCCGTCTCCTT	ORF amplification
51705R	TCAAATGGGATGATTCAACA	ORF amplification
41042F	ATGTTCTTTACTGTTGCTGG	ORF amplification
41042R	TCAAATTCGAAAGCGTTCAA	ORF amplification
828-1305F	TGACCATGGAAATTCCTGCTCGACAATTTGG	Vector construction
828-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCTTCCTCCTCTCGGTTCTCTC	Vector construction
363-1305F	TGACCATGGAACAACAGGAAAAATATCAGCC	Vector construction
363-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCTGTTCTCTTCACCTCGCCCT	Vector construction
081-1305F	TGACCATGGAA ATCGTCAGTGCGCTTCCCCG	Vector construction
081-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCATGCTGTTTCATCATTCCTG	Vector construction
325-1305F	TGACCATGGAA ATTGGCATTGGCCGAACGCA	Vector construction
325-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCATTGAGGCAATCACGCTCCT	Vector construction
687-1305F	TGACCATGGAA GCCAGCTGTGATTCGCCAAA	Vector construction
687-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCGTCACGTTTGGCCAACTGTT	Vector construction
596-1305F	TGACCATGGAA GTGCAATGGCGTGTTGAGCG	Vector construction
596-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCGACCAACGGGTACGACGCCT	Vector construction
445-1305F	TGACCATGGAA CAATCTCCCAATGCAGATAA	Vector construction
445-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCAAAAGCGATGAATGCGACAG	Vector construction
705-1305F	TGACCATGGAA GAACAATACGATTCAACGGA	Vector construction
705-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCAATGGGATGATTCAACAGGA	Vector construction
042-1305F	TGACCATGGAA CTGGGCTGTATGAGCACAAT	Vector construction
042-1305R	TCACACGTGTTACTTATCGTCGTCATCCTTGTAATCAATTCGAAAGCGTTCAATCA	Vector construction

Data	Total reads/bp	Total base pairs/bp	Average length/bp	Length range/bp	N50/bp	N90/bp
Raw reads	33 691 610	4 211 451 250	-	-	-	-
Clean reads>200 bp	33 268 909	4 158 613 625	-	-	-	-
Transcripts	101 595	80 122 468	789	200-15 754	1 284	1 131
Unigenes	72 516	50 829 031	701	200-15 754	311	276

Data	Total reads/bp	Total base pairs/bp	Average length/bp	Length range/bp	N50/bp	N90/bp
Raw reads	33 691 610	4 211 451 250	-	-	-	-
Clean reads>200 bp	33 268 909	4 158 613 625	-	-	-	-
Transcripts	101 595	80 122 468	789	200-15 754	1 284	1 131
Unigenes	72 516	50 829 031	701	200-15 754	311	276

Sequence file	NR	NT	Swiss-Prot	KEGG	PFAM	GO	KOG	ALL
Number	31 374	3 314	24 164	13 521	29 265	29 729	19 183	72 516
Percentage/%	43.26	4.57	33.32	18.64	40.35	40.99	26.45	100

最短尾短体线虫转录组及潜在效应蛋白分析

Transcriptome and Candidate Effectors Analysis of Pratylenchus brachyurus

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CAZy enzyme classes	CAZy families	Number of proteins
Auxiliary activities（AA）	8	64
Glycoside hydrolases（GH）	56	1 285
Polysaccharide lyases（PLs）	4	27
Carbohydrate esterases（CEs）	9	280
Glycosyl transferases（GT）	51	941
Carbohydrate-bind modules（CBMs）	24	1 089
Total	152	3 686

Species	Cellulose		Xylan	Arabinogalactan	Pectin		1,3-Glucan	Chitin
Species	GH5	GH45	GH30	GH53	GH28	PL3	GH16	GH18	GH19	GH20
Pratylenchus brachyurus	85	0	117	2	55	5	56	204	0	17
P. thornei	2	1	2	0	0	2	0	11	2	3
P. coffeae	15	0	1	1	2	4	1	0	0	0
P. zeae	8	0	1	0	0	1	1	5	0	2
P. penetrans	21	0	3	1	4	5	0	10	0	6
Aphelenchoides besseyi	-	+	-	-	-	-	+	-	-	+
A. ritzemabosi	4	7	6	0	0	0	15	68	11	4
Bursaphelenchus xylophilus	0	11	0	0	0	15	6	9	2	7
Globodera pallida	15	0	0	2	0	7	-	-	-	-
Heterodera avenae	16	0	0	0	0	2	1	0	1	1
H. schachtii	24	0	2	3	1	9	0	57	0	5
Meloidogyne incognita	21	0	6	0	2	30	0	3	2	2
M. hapla	6	0	1	0	2	22	0	4	0	1
Caenorhabditis elegans	0	0	0	0	0	0	0	38	6	5

	Caenorhab- ditis elegans	Meloidogyne incognita	Meloidog-yne hapla	Globodera pallida	Heterode-ra avenae	Bursaphelenc-hus xylophilus	Aphelencho-ides besseyi	Pratylenc- hus coffeae	Pratylench- us penetrans	Pratylenchus brachyurus
Small RNA biosynthesis	9	7	6	6	6	8	7	6	7	7
dsRNA uptake and spreading	13	4	4	4	4	6	6	7	4	7
AGOs and RISC	32	9	7	9	8	12	27	14	7	25
RNAi inhibitors	9	2	3	2	3	2	5	1	2	6
Nuclear effectors	15	6	7	5	8	9	12	6	7	9
Total	78	28	27	26	29	37	57	34	27	54

Protein/Gene name	Accession number	Species	Number of unigenes	Best E-value	Bset Identity/%	Bit score	Alignment length/bp
14-3-3/14-3-3b product	AAL40719	Meloidogyne incognita	15	1.34e-138	81.12	386	233
10A06	ACU12489	Heterodera schachtii	0
16D10	Q06JG6	Meloidogyne incognita	0
19C07	AAO85458	Heterodera schachtii	0
Annexin 4C10	AAN32888	Heterodera glycines	6	2.15e-96	45.82	285	323
Calreticulin（Mi-crt-1）	AAL40720	Meloidogyne incognita	6	1.06e-170	82.77	481	354
Cysteine protease（Mi-cpl-1）	CAD89795	Meloidogyne incognita	54	0	80.25	561	324
Fatty acid retinoid binding（Gp-far-1）	CAA70477	Globodera pallida	9	2.73e-83	75.46	241	163
Glutathione-S-transferase（Mi-gsts-1）	ABN64198	Meloidogyne incognita	74	9.84e-92	60.70	263	201
Peroxiredoxin（Gr-TpX）	CAB48391	Globodera rostochiensis	9	9.76e-122	80.81	339	198
RAN-BP-like（Gr-A18）	CAC21848	Globodera rostochiensis	9	4.91e-24	37.21	88.2	129
RAN-BP-like（Gp-rbp-1）	AAV34698	Globodera pallida	14	1.07e-140	81.66	403	229
Glutathione peroxidase	CAD38523	Globodera rostochiensis	0
Transthyretin-like protein（Rs-ttl-1, Rs-ttl-2, Rs-ttl-3, Rs-ttl-4）	CAM84510	Radopholus similis	58	2.27e-64	78.40	190	125
Ubiquitin extension protein（Hs-ubi-1）	AAP30081	Heterodera schachtii	26	2.96e-50	94.74	149	76
Venom allergen-like protein（Vap-1）	AAK60209	Heterodera glycines	4	2.41e-33	50.00	111	118
Venom allergen-like protein（Mi-mps-1）	AAD01511	Meloidogyne incognita	0
7E12	AAQ10021	Meloidogyne incognita	0
Acid phosphatase	AAN08587	Meloidogyne incognita	36	0	56.89	523	450
Chitinase	AAN14978	Heterodera glycines	15	6.17e-69	40.41	219	344
Chorismate mutase 1	ABB02655	Meloidogyne arenaria	1	8.78e-12	34.34	53.5	99
CLE peptide	AAO33474	Heterodera glycines	0
ERp99	AAG21337	Heterodera glycines	9	3.33e-54	75.81	177	124
Galectin	AAB61596	Globodera rostochiensis	27	1.16e-75	60.87	223	161
Map-1	CAC27774	Meloidogyne incognita	0
SPRYSEC（RBP-1）	CAM33004	Globodera pallida	2	3.09e-15	45.16	59.3	62
RING-H2 zinc finger protein	AAP30834	Heterodera glycines	0
SKP1-like protein	AAP30763	Heterodera glycines	8	3.98e-30	35.36	105	181
SXP/RAL-2	CAB75701	Globodera rostochiensis	4	4.87e-17	32.74	65.5	113
Ubiquitin extension protein	AAO33478	Heterodera glycines	1	2.03e-10	33.72	50.4	86
Venom allergen-like protein（VAP-1）	AEL16453	Globodera rostochiensis	8	1.24e-83	68.68	243	182
Effector protein GPP	ARC52277	Meloidogyne graminicola	1	3.99e-17	33.33	70.1	150
Secretory protein（MJ-NULG1a）	AFB73917	Meloidogyne javanica	0
Cellulase（β-1,4-endo glucanase）	CAJ77137	Meloidogyne javanica	30	5.15e-129	72.13	380	302

Protein number	Length /aa	Best matches in Nr	Species	E-value	Query cover/%	Identity /%	Accession Number
c40687_g1	184	Translocon associated protein delta subunit	Pratylenchus goodeyi	4e-80	93	67	AHB64350
c56651_g1	136	Beta-1,4-endoglucanase	Aphelenchus avenae	4e-41	96	51	BAI44493
c40783_g1	638	Matrix metalloproteinase	Globodera rostochiensis	0.0	87	51	AAR11447
c49911_g2	481	Beta-1,4-endoglucanase	Pratylenchus penetrans	0.0	92	75	BAB68522
c23527_g1	78	Transthyretin-like protein 4	Meloidogyne javanica	3e-12	76	52	AKV89653
c61136_g1	88	Beta-1,4-endoglucanase precursor	Globodera rostochiensis	1e-16	64	56	AAC48325
c44079_g2	119	Beta-1,4-endoglucanase	Pratylenchus goodeyi	2e-34	57	81	AJD14760
c40872_g1	241	Lysozyme	Ditylenchus destructor	2e-07	79	28	ADW77529
c50445_g1	493	Acid phosphatase	Heterodera avenae	1e-125	90	43	AFQ55439
c45880_g1	457	Acid phosphatase	Heterodera avenae	2e-94	87	41	AFQ55439
c45363_g1	266	Secreted glutathione peroxidase	Globodera rostochiensis	4e-142	95	75	AHW98769
c35087_g1	383	GHF5 endo-1,4-beta-glucanase precursor	Radopholus similis	7e-107	78	53	ABV54446
c27617_g1	209	C-type lectin	Rotylenchulus reniformis	8e-13	75	31	AFM37305
c50084_g1	268	Secreted glutathione peroxidase	Heterodera cruciferae	3e-114	76	72	AJE31857
c41828_g1	318	SXP/RAL-2 protein	Meloidogyne incognita	1e-23	43	36	AAR35032
c44657_g1	420	Cathepsin L-like cysteine proteinase	Ditylenchus destructor	0.0	93	67	ACT35690
c41337_g1	476	Serine carboxypeptidases	Radopholus similis	0.0	94	58	AIC75882
c35220_g1	242	Glutathione peroxidases	Ditylenchus destructor	5e-129	92	76	AFJ15101
c44519_g1	467	Acid phosphatase	Heterodera avenae	2e-90	97	36	AFQ55439
c39081_g1	240	Putative amphid protein	Globodera rostochiensis	2e-37	66	43	CAB66341
c43667_g1	176	Trans-thyretin-related family domain family member	Aphelenchoides besseyi	2e-46	75	49	AGA60310
c47241_g1	445	Acid phosphatase	Heterodera avenae	4e-95	84	43	AFQ55439
c30155_g1	175	Endoglucanase-2 precursor	Pratylenchus vulnus	4e-38	94	45	CDM79919
c61123_g1	100	Polygalacturonase	Meloidogyne incognita	2e-19	91	47	AAM28240
c50692_g1	664	Heat shock protein 70-C	Ditylenchus destructor	0.0	99	90	AFL69919
c43858_g1	423	Acid phosphatase	Heterodera avenae	2e-70	95	35	AFQ55439
c26240_g1	156	Putative esophageal gland cell secretory protein 21	Meloidogyne incognita	7e-28	99	37	AAN08587
c30506_g1	142	Transthyretin-like protein 2 precursor	Radopholus similis	7e-43	79	58	CAM845111
c50669_g2	288	Serine proteinase	Meloidogyne incognita	8e-92	95	50	ABQ02009
c45343_g9	139	Hypothetical esophageal gland cell secretory protein 11	Heterodera glycines	6e-46	87	65	AAF76925
c45839_g1	357	Biotin synthase	Heterodera glycines	0.0	91	74	ACZ34281
c65044_g1	165	Expansin B	Globodera tabacum	7e-16	32	73	AEU04757
c38872_g2	372	Expansin B	Meloidogyne javanica	2e-09	30	35	ADX36366
c49407_g1	213	VAP1 protein	Globodera rostochiensis	6e-86	96	62	AEL16453
c16433_g1	257	C-type lectin	Rotylenchulus reniformis	2e-11	77	31	AFM37307
c49384_g1	404	Cathepsin L-like cysteine proteinase	Ditylenchus destructor	7e-153	99	58	ACT35690
c25396_g1	154	Acid phosphatase	Heterodera avenae	2e-42	94	51	AFQ55439
c40020_g1	158	Trans-thyretin-related family domain family member	Aphelenchoides besseyi	2e-46	86	50	AGA60310
c19843_g1	108	Gland-specific protein g4e02	Heterodera glycines	3e-16	51	60	AAO33473
c51705_g1	525	Serine carboxypeptidases	Radopholus similis	0.0	87	83	AIC75882
c44522_g1	272	Venom allergen-like protein vap-2	Ditylenchus destructor	1e-48	72	45	ADC35399
c41042_g1	392	Putative esophageal gland cell secretory protein 26	Meloidogyne incognita	6e-105	61	62	AAN15806
c31126_g1	213	Matrix metalloproteinase	Globodera rostochiensis	6e-25	78	37	AAR11447
c49596_g1	394	Putative cathepsin L protease	Meloidogyne incognita	0.0	97	70	CAD89795
c29740_g1	238	Dual oxidase	Meloidogyne incognita	2e-122	82	91	AAY84711
c38325_g1	151	Transthyretin-like protein 1 precursor	Radopholus similis	9e-54	82	73	CAM84510
c42933_g1	363	Arginine kinase	Heterodera glycines	0.0	86	87	AAO49799
c47148_g1	610	Acetylcholinesterase 3	Ditylenchus destructor	0.0	95	70	ABQ58115
c51959_g1	673	Heat shock protein 70-C	Ditylenchus destructor	0.0	94	90	AFL69919
c45122_g1	84	Transthyretin-like protein 4	Meloidogyne javanica	1e-19	84	58	AKV89653
c40147_g1	415	VAP1 protein	Globodera rostochiensis	7e-56	41	52	AEL16453

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