自制DMEM/F12培养基对奶牛乳腺上皮细胞生物学功能的影响

生物技术通报 ›› 2013, Vol. 0 ›› Issue (3): 114-119.

自制DMEM/F12培养基对奶牛乳腺上皮细胞生物学功能的影响

张兴夫¹,杜瑞平²,高民²,敖长金¹,卢德勋²,曹琪娜¹,

1. 内蒙古农业大学动科院，呼和浩特 010018 ；2. 内蒙古农牧业科学院动物营养所，呼和浩特 010031

收稿日期:2012-09-05 修回日期:2013-03-21 出版日期:2013-03-20 发布日期:2013-03-21
作者简介:张兴夫，博士研究生，研究方向：奶牛营养调控理论与技术；E-mail:zhangxingfu666@sina.com
基金资助:
国家“973”计划项目（2011CB100800）

Effects of Self-compounding DMEM/F12 Medium on Biological Function in Primary Cultured Bovine Mammary Epithelial Cells

Zhang Xingfu¹, Du Ruiping², Gao Min², Ao Changjin¹, Lu Dexun², Cao Qina1

1. Inner Mongolia Agricultural University,Huhhot 010018 ；2. Animal Nutrition Institute of Agriculture and Animal Husbandry Academy of Inner Mongolia,Huhhot 010031）

Received:2012-09-05 Revised:2013-03-21 Published:2013-03-20 Online:2013-03-21

摘要/Abstract

摘要： 验证自制DMEM/F12 培养基与商品化DMEM/F12 培养基相比是否会对奶牛乳腺上皮细胞生物学功能造成负面影响，为进一步研究氨基酸对乳蛋白合成影响及其机理奠定基础。从对奶牛乳腺上皮细胞增殖能力、泌乳相关基因表达和酪蛋白合成这几个方面比较自制DMEM/F12 培养基与DMEM/F12 培养基的差别。结果显示，自制DMEM/F12 培养基对乳腺上皮细胞的增殖活力要显著高于DMEM/F12 培养基（P < 0.0001）；自制DMME/F12 培养基与商品化DMEM/F12 培养基相比乳腺上皮细胞CSN1S1、CSN3 基因表达显著上调（P<0.05），上调量分别达到了3.57 倍和3.27 倍，CSN1S2、CSN2 基因表达量差异不显著（P >0.05）；自制 DMEM/F12 培养基与商品化DMEM/F12 培养基相比，对乳腺上皮细胞αS酪蛋白及β-酪蛋白的合成的影响差异并不显著（P >0.05）。由此得出，与商品化DMEM/F12 培养基相比，自制DMEM/F12 培养基在奶牛乳腺上皮细胞增殖、泌乳相关基因表达和酪蛋白合成方无负面影响。

关键词: 自制DMEM/F12, 培养基, 基因表达, 酪蛋白, 细胞增殖

Abstract: The purpose of this study was to exam if the self-compounding medium had negative effects on the biological function of bovine mammary epithelial cells and offered foundation for further experiments.The differences between self-compounding medium and DMEM/F12 medium in this experiment were compared based on determination of cell proliferation, lactating genes expression and casein synthesis. The proliferation of BMEC cultured in self-compounding medium was higher significantly than that of DMEM/F12 medium（P < 0.0001）. The CSN1S1 and CSN3 genes expression of BMEC cultured in self-compounding medium increased significantly（P < 0.05）and up-regulated 3.57-fold and 3.27-fold, respectively. There were no significance of the CSN1S2, CSN2 gene expression and αS-casein, β-casein synthesis（P >0.05）.Compared with DMEM/F12 medium, self-compounding medium had no negative effects on the lactating genes expression and the casein synthesis, and the further study on effects of different amino acid patterns on lactating genes expression and casein synthesis.

Key words: Self-compounding DMEM/F12 medium, Lactating gene expression, Casein synthesis, Cell proliferation

张兴夫,杜瑞平,高民,敖长金,卢德勋,曹琪娜,. 自制DMEM/F12培养基对奶牛乳腺上皮细胞生物学功能的影响[J]. 生物技术通报, 2013, 0(3): 114-119.

Zhang Xingfu, Du Ruiping, Gao Min, Ao Changjin, Lu Dexun, Cao Qina. Effects of Self-compounding DMEM/F12 Medium on Biological Function in Primary Cultured Bovine Mammary Epithelial Cells[J]. Biotechnology Bulletin, 2013, 0(3): 114-119.

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