Songjiang Sea Bass(Trachidermus fasciatus)Analysis of Gene Cloning and Expression of Coagulation Factor XI

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.029

Abstract

Abstract: It was to clone the full length cDNA encoding coagulation factor XI(FXI)of Trachidermus fasciatus. A TfXI gene from Trachidermus fasciatus(TfXI)was cloned and characterized by RACE technology. The TfXI cDNA composed of 1 287 bp with a 13 bp of 5'-UTR, 1 143 bp open reading frame(ORF)and 131 bp 3'-UTR, encoded a polypeptide of 280 amino acids. Sequence alignment of TfXI showed the highest similarity of 63% with Haplochromis burtoni FXI protein. After LPS stimulation, transcripts of TfXI were significantly increased and reached to peak at 96 h p.i. It indicated that TfXI may play an important role in immune response of T. fasciatus during pathogen challenge.

Key words: Trachidermus fasciatus, coagulation factor XI, cloning, real time PCR

Qi Qi, Qi Yunyue, Yang Hui, Bi Caihong, Han Xiaodi. Songjiang Sea Bass(Trachidermus fasciatus)Analysis of Gene Cloning and Expression of Coagulation Factor XI[J]. Biotechnology Bulletin, 2015, 31(3): 199-206.

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