Cloning and Bioinformatics Analysis of euFUL Gene in Panax ginseng

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.019

Abstract

Abstract: To study the flower development of Panax ginseng, designing specific primers according to gene fragments in the GenBank data, and the 5' end deletion coding sequence of a euFUL(named PgFUL)gene in ginseng flower was cloned by the rapid amplification of cDNA ends(RACE). PgFUL consists of 867 nucleotides, and encoding a protein of 212 amino acids. The bioinformatics analysis shows that PgFUL-encoding protein has isoelectric point(pI)of 5.80 and a calculated molecular weight about 24.64 kD without transmembrane region and signal peptide. In the secondary structure, the percentage of α-helix, extended strand and random coil are 60.19%, 5.21% and 34.60%, respectively. The homologous analysis by sequence alignment and phylogenetic indicates that PgFUL is in 100% similarity with ginseng PgMADS protein3(BAK20018.1)and 95% with Menispermum canadense(AGX01589.1), which belongs to evolutionary euFUL in functional gene A.

Key words: euFUL gene, bioinformatics analysis, gene cloning, Panax ginseng

Guo Shuangshuang, Liu Cuijing, Han Mei, Yang Limin. Cloning and Bioinformatics Analysis of euFUL Gene in Panax ginseng[J]. Biotechnology Bulletin, 2015, 31(6): 122-128.

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