Functional Verification and Bioinformatics Analysis of Cotton GhDMT3 Gene

doi:10.13560/j.cnki.biotech.bull.1985.2018-0724

Abstract

Abstract: The function of the gene in cotton and higher plants was explored by building VIGS silencing vector of GhDMT3 and infecting cotton. Bioinformatics was used to analyze the structure characteristics of DNA sequence of cotton DNA methyltransferase gene GhDMT3（CotAD_46796）as well as the physicochemical properties,hydrophobicity,and secondary structure of the protein encoded by this gene. The gene was cloned and the pYL156：GhDMT3 silencing vector was constructed. The relative molecular weight of the protein was 71 107.02 kD,the pI was 4.85,and the instability coefficient was 36.33. Cotton seedlings of VIGS silenced GhDMT3 treated with different stress（cold and dry）showed significant phenotypic differences. The expression pattern analysis of qRT-PCR showed that the expression levels in the different parts of pYL156-infected cotton did not vary under different stress,while the transcriptional Level of GhDMT3 in pYL156：GhDMT3-infected cotton decreased significantly compared with the control. Compared with cotton seedlings without GhDMT3 silenced,cotton seedlings with GhDMT3 silenced were significantly resistant to stress.

Key words: Gossypium hirsutum L., GhDMT3, bioinformatics analysis, VIGS silencing

YANG Xiao-min, WANG Jun-juan, WANG De-Long, LU Xu-ke, CHEN Xiu-gui, GUO Li-xue, WANG Shuai, CHEN Chao, WANG Xiao-ge, HAN Ming-ge, YE Wu-wei. Functional Verification and Bioinformatics Analysis of Cotton GhDMT3 Gene[J]. Biotechnology Bulletin, 2019, 35(1): 11-16.

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