Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (8): 106-117.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0247
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NIE Zhu-xin1(), GUO Jin1, QIAO Zi-yang1, LI Wei-wei1, ZHANG Xue-yan1, LIU Chun-yang1, WANG Jing1,2()
Received:
2024-03-14
Online:
2024-08-26
Published:
2024-07-31
Contact:
WANG Jing
E-mail:1538379214@qq.com;wangjing_imu@163.com
NIE Zhu-xin, GUO Jin, QIAO Zi-yang, LI Wei-wei, ZHANG Xue-yan, LIU Chun-yang, WANG Jing. Transcriptome Analysis of the Anthocyanin Biosynthesis in the Fruit Development Processes of Lycium ruthenicum Murr.[J]. Biotechnology Bulletin, 2024, 40(8): 106-117.
基因ID Gene ID | GenBank登录号GenBank number | 引物序列Primer sequence(5'-3') | 扩增长度Amplification length/bp |
---|---|---|---|
Cluster-53530 | — | F: ACCGATGAAGAACTCGTCGTCCA | 228 |
R: GGTAGCCTTCCAATAACCCGATGT | |||
Cluster-54040 | KY287796 | F: CTAAATGCCCCCAACCAGAACT | 124 |
R: GTTACCCACTTCCCTTCATAGA | |||
Cluster-55329 | OQ414189 | F: TTTTGGACTGCTGGTTCTTGTT | 260 |
R: TTGAGGTCTTTGTTGATTATCGG | |||
Cluster-57771 | — | F: CAAGGAGGAAATGCAAAGAGGAGC | 254 |
R: CTATTAGGGACCATCTGTTGCCAAG | |||
Cluster-59360 | — | F: CCGATGTAACGGATGGTGTCTA | 182 |
R: GGGGCTGATTTGTCTCTATTGC | |||
Cluster-67674 | KY287799 | F: AGAATGGGCACTGGCAGAAATGAT | 210 |
R: ACTACACACGGCTCGTTTGATACC | |||
Cluster-67748 | KF031378 | F: TCCCTTTTCCTTCCGAGTTCAT | 164 |
R: TGTTTTGCCCTTCCACTGCTGC | |||
Cluster-73437 | KY287797 | F: CACGGTAAGGAGACTGGTTTTCA | 167 |
R: CAGCCTTCTCTGCCAGTATCTTG | |||
Cluster-74326 | KY287798 | F: CAGTGGTGAACTCGGATAGCAGCA | 117 |
R: GCTCCGCCATTACTGCTTTCTCTC | |||
Cluster-76366 | — | F: GCAATCTCTTGTTCCTGGTTGTGAC | 288 |
R: GGATACCCGCTGTTAGTGTTAGGAA | |||
LrH2B1 | — | F: AGTGCTTCCTGGTGAATTGG | 163 |
R: TGGATAATACCTAGCCCTAGTTTCC |
Table 1 Primer sequences used in this study
基因ID Gene ID | GenBank登录号GenBank number | 引物序列Primer sequence(5'-3') | 扩增长度Amplification length/bp |
---|---|---|---|
Cluster-53530 | — | F: ACCGATGAAGAACTCGTCGTCCA | 228 |
R: GGTAGCCTTCCAATAACCCGATGT | |||
Cluster-54040 | KY287796 | F: CTAAATGCCCCCAACCAGAACT | 124 |
R: GTTACCCACTTCCCTTCATAGA | |||
Cluster-55329 | OQ414189 | F: TTTTGGACTGCTGGTTCTTGTT | 260 |
R: TTGAGGTCTTTGTTGATTATCGG | |||
Cluster-57771 | — | F: CAAGGAGGAAATGCAAAGAGGAGC | 254 |
R: CTATTAGGGACCATCTGTTGCCAAG | |||
Cluster-59360 | — | F: CCGATGTAACGGATGGTGTCTA | 182 |
R: GGGGCTGATTTGTCTCTATTGC | |||
Cluster-67674 | KY287799 | F: AGAATGGGCACTGGCAGAAATGAT | 210 |
R: ACTACACACGGCTCGTTTGATACC | |||
Cluster-67748 | KF031378 | F: TCCCTTTTCCTTCCGAGTTCAT | 164 |
R: TGTTTTGCCCTTCCACTGCTGC | |||
Cluster-73437 | KY287797 | F: CACGGTAAGGAGACTGGTTTTCA | 167 |
R: CAGCCTTCTCTGCCAGTATCTTG | |||
Cluster-74326 | KY287798 | F: CAGTGGTGAACTCGGATAGCAGCA | 117 |
R: GCTCCGCCATTACTGCTTTCTCTC | |||
Cluster-76366 | — | F: GCAATCTCTTGTTCCTGGTTGTGAC | 288 |
R: GGATACCCGCTGTTAGTGTTAGGAA | |||
LrH2B1 | — | F: AGTGCTTCCTGGTGAATTGG | 163 |
R: TGGATAATACCTAGCCCTAGTTTCC |
Fig. 1 Phenotypes(A)and anthocyanin contents(B)of fruit at different developmental stages of L. rutheni-cum Murr. BS1: Green fruit stage. BS2: Early color-changing stage. BS3: Late color-changing stage. BS4: Ripeness stage. BS5: Complete ripeness stage. Using BS1 as a control, ** P < 0.01, *** P < 0.001. The same below
Fig. 3 Gene ontology annotation of DEGs in the fruits at different stages of L. ruthenicum Murr. Green: Biological process. Gray: Cell component. Purple: Molecular function
Fig. 6 Candidate genes related to anthocyanin biosynthesis in KEGG pathway and their correlation with anthocyanins R2: Correlation between gene expression(FPKM)and anthocyanin content. The same below
Fig. 7 Heat map of expressions of key genes in plant hormone signal transduction pathway A: ABA signal transduction pathway. B: GA signal transduction pathway. C: JA signal transduction pathway
Fig. 8 Validation of transcriptome data Using BS1 as a control, * P < 0.05, ** P < 0.01, and *** P < 0.001. R2: Correlation between transcriptome data(RNA-seq)and RT-qPCR result(RT-qPCR)
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