Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (8): 106-117.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0247

Previous Articles     Next Articles

Transcriptome Analysis of the Anthocyanin Biosynthesis in the Fruit Development Processes of Lycium ruthenicum Murr.

NIE Zhu-xin1(), GUO Jin1, QIAO Zi-yang1, LI Wei-wei1, ZHANG Xue-yan1, LIU Chun-yang1, WANG Jing1,2()   

  1. 1. College of Biological Science and Engineering, North Minzu University, Yinchuan 750021
    2. Innovation Team for Genetic Improvement of Economic Forests, North Minzu University, Yinchuan 750021
  • Received:2024-03-14 Online:2024-08-26 Published:2024-07-31
  • Contact: WANG Jing E-mail:1538379214@qq.com;wangjing_imu@163.com

Abstract:

【Objective】To explore the molecular mechanism involved in anthocyanin biosynthesis during the fruit development of Lycium ruthenicum Murr. is important for deeply understanding the regulatory network of anthocyanin biosynthesis.【Method】Transcriptome sequencing was performed on the samples at the five stages of fruit development: green fruit, early color-changing, late color-changing, ripeness, and complete ripeness. The candidate genes related to anthocyanin biosynthesis were mined.【Result】With the development of L. ruthenicum Murr. fruit, the anthocyanin content gradually increased. The anthocyanin content in the fruits at the ripeness and complete ripeness stages were significantly higher than that at the green fruit, early color-changing, and late color-changing stages. A total of 13 540 differentially expressed genes(DEGs)were identified from the five stages of fruit development. Using the green fruit stage as a control, the number of DEGs gradually increased with the development of the fruit. GO analysis showed that DEGs were co-enriched GO terms including phenylpropanoid metabolic process, polysaccharide catabolic process, phenylpropanoid biosynthetic process, flavonoid biosynthetic process, cell wall macromolecule metabolic process, anchored component of membrane, and nutrient reservoir activity. KEGG analysis indicated the significant enrichment of DEGs in flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, stilbenoid, diarylheptanoid and gingerol biosynthesis, as well as cutin, suberine, and wax biosynthesis. The correlation between DEGs expression and anthocyanin content was performed and 36 candidate genes involved in anthocyanin biosynthesis during the L. ruthenicum fruit development were screened, including ten structural genes of anthocyanin biosynthesis pathway, four genes of transcription factors, nine, eight, and five genes of ABA, GA, and JA signal transduction pathway. Ten DEGs were selected for RT-qPCR analysis, and the results were consistent with RNA-seq data.【Conclusion】DEGs related to structural genes and transcription factors involved in anthocyanin biosynthesis, as well as genes involved in the ABA, GA, and JA signal transduction pathways, affect fruit coloration during the development of L. ruthenicum Murr. fruit.

Key words: Lycium ruthenicum Murr., fruit, transcriptome, differentially expressed gene, plant hormone, regulation of anthocyanin biosynthesis