Research of Reversal Effect on K562/A02 Cell Line with a Novel Marine Bioactive Substances

Abstract

Abstract: This test was designed to study the effects of FAT on multidrug resistance（MDR）in human leukemia K562/A02 cells. MTT method was used to observe the drug sensitivity and the effect of FAT on the drug resistance; Flow cytometer was used to measure the intracellular drug accumulation; Cellular GSH concentration was examined by biochemical analyses with DTNB. Results showed that FAT did not exhibit an inhibitory activity to proliferation in K562 cell line and K562/A02 cell line. FAT decreased IC50 of ADM in K562/A02 cell line. And it had no remarkable effect on K562 cell line. FAT increased the intracellular Rho-123 concentration. Cellular GSH concentration in K562/ A02 cell line was higher than thatin K562 cell line. FAT decreased cellular GSH concentration in K562/A02 cell line. One of the mechanisms of multidrug resistance reversal by FAT was decrease of cellular GSH concentration in K562/A02 cell line.

Key words: Multidrug resistance, FAT, Glutathione

Zhang Yuxia, Liang Qiong, Yong Guoxin. Research of Reversal Effect on K562/A02 Cell Line with a Novel Marine Bioactive Substances[J]. Biotechnology Bulletin, 2013, 0(2): 177-183.

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