Abstract: In order to investigate the embryo protein expression of the wheat seeds germination, tow-dimensionsl gel electrophoresis （2-DE） technology was used to separated proteins from ungerminated seed and embryo buds of germinated seed at specific stages of 3h, 16h, 25h in wheat “Jinmai-47”. The results showed that on the 2-DE gels PDQuest image software detected about 127, 131, 135, 141 protein spots, of which 17 spots show more than 2.5-fold changes in abundance among ungerminated seed and embryo buds of germinated seed at specific stages of 3 h, 16 h, 25 h in wheat “Jinmai-47”. These 9 of 17 protein spots treated by mass spectrometry analysis, of which 4 differential proteins were elementarily identified as nucleoside two phosphoglycerate kinase Ⅰ , 17.5 kD heat shock protein, ATP synthase and LEA protein 27.

Key words: Wheat, Embryo, Two-dimensional electrophoresis, Mass spectrometry technology