Cloning and Bioinformation Analysiss of StSnRK2.1 Gene in Solanum tuberosum

Abstract

Abstract: SnRK2s plays an important role in the regulation when plants suffer from adervise environments. The full-length cDNA sequence of SnRK2.1 in Solanum tuberosum was successfully cloned by RT-PCR with the tube plant. The gene was named StSnRK2.1 and has been submitted to Genbank with the accession number JX280911.By bioinformation analysis, the results showed that the StSnRK2.1 has a complete ORF sequence is 1 008 bp, encoding 335 predicted amino acids. The protein molecular weight is 37.77 kD and theoretical pI is 5.37. In protein’s secondary structure, Alpha helix is 42.39%, Extended strand 16.42%, Bela turn is 7.46%, Random coil is 33.73%. Also, the protein is drophilicity, haven’t transmembrane. It is probably exit in the cytoplasm and microbody by subcellular localization prediction of protein. There are seven serine sites, two threonine sites, three tyrosine sites, so it is putative that the StSnRK2.1 is related to plant’s tolerance in abiotic stress.

Key words: Solanum tuberosum, Anti-drought, Cloning, Bioinformation analysis

Liu Siyan, Mao Juan, Fan Aqi, Zhang Junlian, Wang Di, Bai Jiangping. Cloning and Bioinformation Analysiss of StSnRK2.1 Gene in Solanum tuberosum[J]. Biotechnology Bulletin, 2013, 0(3): 83-89.

References

[1] Umezawa T, Yoshida R. SRK2C, a SNF1-related protein kinase 2, improves drought tolerance by controlling stress-responsivegene expression in Arabidopsis thaliana[J] . PNAS, 2004, 101(49), 17306-17311.
[2] Boudsocq M, Lauriere C. Osmotic signal in plants multiple pathways mediated by emerging kinase families [J] .Plant Physiology, 2005, 138 :1185-1194.
[3] Kobayashi Y, Yamamoto S, Minami H, et al. Differential activation of the rice sucrose non fermenting l-related protein kinase2 family by hyperosmotic stress and abscisic[J].The Plant Cell, 2004, 16 : 1163-1177.
[4] Huai JL, Wang M, He JG, et al. Cloning and characterization of the SnRK2 gene family from Zea mays[J]. Plant Cell Reports, 2008, 27 :1861-1868.
[5] Cohen P. Protein phosphorylation and hormone action[J]. Proceedings of the Royal Society B.Biological Sciences, 1988, 234 :115- 144.
[6] Jaglo-Ottosen KR, Gilmour SJ, ZaRKa DG et al. Arabidopsis CBF1 over expression induces COR genes and enhances freezing tolerance[J]. Science, 1998, 280(5360):104-106.
[7] Passioura JB. Environmental biology and crop improvement[J]. Functional Plant Biology, 2002, 29 :537-546.
[8] Ingram J, Bartels D. The molecular basis of dehydration tolerance in plants[J]. Annu Rev Plant Physiol Plant Mol Biol, 1996, 47 : 377-403.
[9] Schafleitner R, Gutierrez Rosales RO, Gaudin A. Capturing candidate drought tolerance traits in two native Andean potato clones by transcription profiling of field grown plants under water stress[J]. Plant Physiol Biochem, 2007, 45(9):673-690.
[10] Fujita Y, Fujita M, Satoh R, et al. AREB1 Is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis[J]. Plant Cell, 2005, 17(12): 3470-3488.
[11] Karim S, Aronsson H, Ericson H, et al. Improved drought tolerance without undesired side effects in transgenic plants producing trehalose [J]. Plant Mol Biol, 2007, 64(4):371-386.
[12] Gosal SS, Wani SH, Kang MS. Biotechnology and drought tolerance. Journal of Crop Improvement, 2009, 23 :19-54.
[13] 唐先兵, 赵恢武, 林忠平. 植物耐旱基因工程研究进展[J]. 首都师范大学学报:自然科学版, 2009, 23(3):47-51.
[14] 李娜, 李莉, 陈光辉. 作物抗旱机理及其相关基因的研究进展[J]. 长江大学学报:自然科学版, 2011, 8(3):239-243.
[15] Chaves MM, Pereira J, Maroco J. How plants cope with water stress in the field?[J]. Photosynthesis and Growth. Ann Bot, 2002, 89 (7):907-916.
[16] Xu ZZ, Zhou GS, Shimizu H. Plant responses to drought and rewatering [J]. Plant Signaling & Behavior, 2010, 5(6):649-654.
[17] 张树珍, 王自章. 物耐旱的分子基础及植物耐旱基因工程的研究进展[J]. 生命科学研究, 2001, 5(3):134-140.
[18] 刘欣, 李云. 转录因子与植物抗逆性研究进展[J]. 中国农学通报, 2006, 22 (4): 61-65.
[19] Sambrook J, Fritsh EF, Maniatis T. 分子克隆实验指南
[ M] . 第 2 版. 北京:科学出版社, 1992.
[20] 吴峻岩, 王茂广, 吴能表, 等. 植物中的CDPK/SnRK 蛋白激酶家族[J]. 激光生物学报, 2004, 13(4):265-269.
[21] 王永波, 高世庆, 唐益苗, 等. 植物蔗糖非发酵. 1 相关蛋白激酶家族研究进展[J]. 生物技术通报, 2010(11):7-17.
[22] 李利斌, 开昌, 李现刚. 三个新的玉米SnRK2 基因的鉴定和特征分析[J]. 山东农业科学, 2009, 12 :7-11.
[23] Amaguehi K, Shinmkik K. Transcriptional regulatory networks incellular responses and tolerance to dehydraliotl and cold stress). Annu Rev Plant Bio, 2006, 57 :781-803.
[24] 连魏卫, 唐益苗, 高世庆, 等. 小麦TaSnRK2.9 蛋白激酶基因克隆与生物信息学分析[J]. 中国农学通报, 2011 , 27(33): 6-12.
[25] 徐蓓, 郭丽香, 赵昌平, 等. 长穗偃麦草EeSnRK2.6 基因克隆及生物信息学分析[J]. 麦类作物学报, 2012, 32(1):36- 43.