Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (4): 87-90.

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The Polymorphism of MC1R Gene in Yak

Chen Si1, Chai Zhixin1，2, Wang Yong1，2, Zhong Jincheng1，2,   

  1. (1. Key Laboratory of Animal Genetics and Breeding，Southwest University for Nationalities，State Ethnic Affairs Commission and Ministry of Education，Chengdu 610041；
    2. Institute of Tibetan Plateau Research，Southwest University for Nationalities，Chengdu 610041)
  • Received:2013-11-13 Online:2014-04-29 Published:2014-04-29

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Abstract: It was to explore the relationship between MC1R genotype and the coat color formation in yak, polymorphism of MC1R gene was detected by PCR-SSCP and DNA sequencing techniques in 64 yaks(33 white coat color and 31 black coat color). The results showed that there were polymorphisms in the amplified region for the Tianzhu yak and Jiulong yak. In Tianzhu yak and Jiulong yak, three genotypes(AA, BB and AB)were detected. The result of χ2, three yak breeds were not fit for Hardy-Weinberg law. Sequencing revealed on single nucleotide mutation(C→A)at 179bp and(T→C)at 214 bp of the amplified region of Tianzhu yak and Jiulong yak.

Key words: Yak, MCIR gene, PCR-SSCP, Polymorphism