Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (9): 189-194.

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Analysis of the Relationship Between Polymorphism of Leptin and Seasonal Estrous in Sheep

Li Hui1,2,3,Lu Shouliang1,2,3,Wan Pengcheng1,3,Dai Rong1,3,Li Liangyuan1,2,3,Meng Haohao1,2,3,Shi Guoqing1,3   

  1. 1. Institute of Animal Husbandry and Veterinary，Xinjiang Academy of Agricultural and Reclamation Sciences，Shihezi University，Shihezi 832000;
    2. College of Animal Science and Technology，Shihezi 832003;
    3. Key Lab of Sheep Breeding and Reproduction，Xinjiang Agroreclamation Academy of Sciences，Shihezi 832000
  • Received:2014-02-02 Online:2014-09-15 Published:2014-09-07

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Abstract: According to the known sequence of leptin gene from GenBank, two primers were designed. The single nucleotide polymorphisms（SNPs）of the nonseasonal estrus Hu sheep and seasonal estrus Altay sheep were detected using PCR-SSCP assay. Then, the screening SNPs was subjected to the relative analysis of genotype and seasonal estrus. The results showed that three consecutive bases TTG inserted and C / T mutated intron 1 of leptin in Altay sheep, and G / T mutation in exon 3 resulted in the replacement of valine by leucine in contrast with the Hu sheep. Three genotypes AA, AB, BB were detected on the amplified fragment of the exon 2, the genotype BB was dominant in Altay. The genotype distributions were significantly different（P<0.001）between two groups, especially demonstrated that genotype BB was benefit for seasonal estrus. In conclusion, the sequence difference of Leptin in different sheep breeds may be one possible reason leading to nonseasonal or seasonal estrus, which can be used as an assistant marker for nonseasonal estrus breeding.

Key words: Sheep , Leptin , Seasonal estrous , PCR-SSCP