Advances in Bacterial Multiplex Genome Engineering and Its Applications in Synthetic Biology

doi:10.13560/j.cnki.biotech.bull.1985.2018-0089

Abstract

Abstract: Multiplex genome engineering refers to the technology that can simultaneously edit multiplex genome targets in one cell by a single experiment. High-throughput is a key direction of gene editing technologies,and the occurrence and development of multiplex genome engineering is an important intermediate stage of high-throughput genome engineering. So far there are mainly three strategies for multiplex genome engineering in bacteria,including iteration based editing,CRISPR/Cas9-based newly developed high-efficiency tools editing,and large synthetic DNA-based editing. In this article,the principles and developments of these three kinds of multiplex genome engineering technologies,as well as their applications in synthetic biology of microorganism are reviewed,and the shortcoming of these technologies are discussed. Besides,we propose the future development directions of multiplex genome engineering.

Key words: bacterial genome engineering, multiplex genome engineering, synthetic biology

GUO Ming-zhang, LIU Hai-yan, DU Ruo-xi, XU Wen-tao. Advances in Bacterial Multiplex Genome Engineering and Its Applications in Synthetic Biology[J]. Biotechnology Bulletin, 2018, 34(5): 22-31.

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