Effects of Ethanol on Metabolites and Bacterial Community of Rice Straw Cocultured with Rumen Fluid in vitro

doi:10.13560/j.cnki.biotech.bull.1985.2019-1101

Abstract

Abstract: This study aims to study the effect of ethanol addition on rice straw anaerobic fermentation co-cultured with rumen fluid. In vitro fermentation technique by frequent passage and high-throughput sequencing technology were applied to analyze short chain fatty acid（SCFA）yield and bacterial community composition. The results showed that SCFA yield in the rice straw fermentation by in vitro 8^th transferring were significant higher than that by rumen fluid（P<0.01）. Compared with no adding ethanol,ethanol addition significantly increased the proportion of acetate,valerate,and caporate,while decreased propoinate and butyrate proportion（P<0.01）;the total SCFA yield,the proportion of isobutyrate and isovelarate varied insignificantly. The relative abundance of Bacteroidetes phylum decreased,while that of Firmicutes phylum increased by anaerobic fermentation of rice straw（P<0.05）,compared with that by rumen fluid. Furthermore,the relative abundances of Firmicutes and Actinobacteria phyla,Bifidobacterium,unidentified Lachnospiraceae,Succiniclasticum,Desulfovibrio and unidentified Clostridiales genera increased while ethanol was added（P<0.05）. Adding ethanol increased Biomarkers in the rice straw fermentation. The rice straw fermentation and rumen fluid presented close genetic relationship,and adding ethanol significantly changed the bacterial community structure. The proportion of SCFA played an important role in bacterial community of the rice straw fermentation. Our results indicate that in vitro frequent passage and adding ethanol increase the proportion of acetate,valerate,and caporate in rice straw fermentation as ethanol changes the bacterial community of rice straw fermentation.

Key words: ethanol, rumen fluid, rice straw, short chain fatty acid, bacterial community

LIN Miao, WANG Kuo-peng, CHEN Ying-liang, SUN Wen-jing, FENG Li-mei, HU Zi-xuan. Effects of Ethanol on Metabolites and Bacterial Community of Rice Straw Cocultured with Rumen Fluid in vitro[J]. Biotechnology Bulletin, 2020, 36(2): 91-99.

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