Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (4): 85-95.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0550
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WEI Chang1(), QI Xiu-xiu1, WU Yue2, LIU Xiao-dan1, WANG Yi1, JIANG Ying1(), LIU Hai-tao1()
Received:
2020-05-08
Online:
2021-04-26
Published:
2021-05-13
Contact:
JIANG Ying,LIU Hai-tao
E-mail:wyjyl1009@163.com;JY27486@163.com;liuhaitaoky@henau.edu.cn
WEI Chang, QI Xiu-xiu, WU Yue, LIU Xiao-dan, WANG Yi, JIANG Ying, LIU Hai-tao. Screening,Identification,Condition Optimization and Application of Efficient Phosphate Solubilizing Bacteria in Sandy Fluvo Aquic Soil[J]. Biotechnology Bulletin, 2021, 37(4): 85-95.
Fig.1 Phosphate solubilizing efficiency of strains The error line in the figure refers to the standard deviation. The lowercase letters indicate that the difference among different phosphate-solubilizing bacteria reached a significant level (P < 0.05). The same below
项目Item | 结果Results | 项目Projects | 结果Results | |
---|---|---|---|---|
革兰氏染色 Gram staining | + | 淀粉水解 Starch hydrolysis | + | |
好氧性实验 Aerobic test | 兼性厌氧 Facultative anaerobic | 柠檬酸盐利用 Citrate utilization | - | |
接触酶实验 Catalase test | + | 硝酸盐还原 Nitrate reduction | + | |
甲基红(M.R)反应Methyl red reaction | - | V-P实验 V-P test | + |
Table 1 Physiological characteristics of strain W6
项目Item | 结果Results | 项目Projects | 结果Results | |
---|---|---|---|---|
革兰氏染色 Gram staining | + | 淀粉水解 Starch hydrolysis | + | |
好氧性实验 Aerobic test | 兼性厌氧 Facultative anaerobic | 柠檬酸盐利用 Citrate utilization | - | |
接触酶实验 Catalase test | + | 硝酸盐还原 Nitrate reduction | + | |
甲基红(M.R)反应Methyl red reaction | - | V-P实验 V-P test | + |
Fig. 3 Phylogenetic tree that based on 16S rDNA sequences of strain W6 and related strains Each scale refers to that there are two nucleoside substitutions per 1 000 nucleosides
处理Treatments | 不同提取态磷素含量Contents of phosphorus in different extraction states/(g·kg-1) | 回收率 Recovery rate | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
H2O-P | NaHCO3-Po | NaHCO3-Pi | NaOH-Po | NaOH-Pi | HCl-P | 浓HCl-Po Concentrated HCl-Po | 浓HCl-Pi Concentrated HCl-Pi | 浓H2SO4-P Concentrated H2SO4-P | ||
CK | 0.11±0.02 | 0.35±0.10 | 0.22±0.00 | 0.17±0.02 | 0.15±0.03 | 1.56±0.07 | 0.74±0.07* | 0.57±0.04* | 0.09±0.02 | 98% |
W6 | 0.25±0.00** | 0.63±0.10** | 0.34±0.02 | 0.19±0.01 | 0.18±0.02 | 1.39±0.16 | 0.57±0.12 | 0.33±0.09 | 0.06±0.01 | 96% |
Table 2 Effects of innoculated strain W6 on the forms of phosphorus
处理Treatments | 不同提取态磷素含量Contents of phosphorus in different extraction states/(g·kg-1) | 回收率 Recovery rate | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
H2O-P | NaHCO3-Po | NaHCO3-Pi | NaOH-Po | NaOH-Pi | HCl-P | 浓HCl-Po Concentrated HCl-Po | 浓HCl-Pi Concentrated HCl-Pi | 浓H2SO4-P Concentrated H2SO4-P | ||
CK | 0.11±0.02 | 0.35±0.10 | 0.22±0.00 | 0.17±0.02 | 0.15±0.03 | 1.56±0.07 | 0.74±0.07* | 0.57±0.04* | 0.09±0.02 | 98% |
W6 | 0.25±0.00** | 0.63±0.10** | 0.34±0.02 | 0.19±0.01 | 0.18±0.02 | 1.39±0.16 | 0.57±0.12 | 0.33±0.09 | 0.06±0.01 | 96% |
处理Treatment | 根长Root length/cm | 根表面积Root surface area/cm2 | 根体积Root volume/cm3 | 根尖数Number of root shoots/个 | 根直径Root diameter/cm |
---|---|---|---|---|---|
CK | 584.58±58.81 | 43.73±1.40 | 0.26±0.04 | 2578±71.91 | 0.22±0.15 |
W6 | 975.06±22.21** | 68.66±1.75** | 0.39±0.02** | 2929±32.19* | 0.24±0.02* |
Table 3 Effects of strain W6 on the root system of wheat
处理Treatment | 根长Root length/cm | 根表面积Root surface area/cm2 | 根体积Root volume/cm3 | 根尖数Number of root shoots/个 | 根直径Root diameter/cm |
---|---|---|---|---|---|
CK | 584.58±58.81 | 43.73±1.40 | 0.26±0.04 | 2578±71.91 | 0.22±0.15 |
W6 | 975.06±22.21** | 68.66±1.75** | 0.39±0.02** | 2929±32.19* | 0.24±0.02* |
处理 Treatment | 干重 Dry weight/g | 株高 Plant height/cm | 全氮 Total nitrogen/(g·kg-1) | 全磷 Total phosphorus/(g·kg-1) | 全钾 Total potassium/(g·kg-1) |
---|---|---|---|---|---|
CK | 1.46±0.07 | 16.52±1.68 | 1.86±0.17 | 1.71±0.56 | 6.68±0.58 |
W6 | 2.49±0.77** | 21.83±0.31* | 2.32±0.06* | 3.32±0.55** | 8.98±0.19** |
Table 4 Effects of strain W6 on wheat
处理 Treatment | 干重 Dry weight/g | 株高 Plant height/cm | 全氮 Total nitrogen/(g·kg-1) | 全磷 Total phosphorus/(g·kg-1) | 全钾 Total potassium/(g·kg-1) |
---|---|---|---|---|---|
CK | 1.46±0.07 | 16.52±1.68 | 1.86±0.17 | 1.71±0.56 | 6.68±0.58 |
W6 | 2.49±0.77** | 21.83±0.31* | 2.32±0.06* | 3.32±0.55** | 8.98±0.19** |
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