Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (9): 258-263.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1177

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Effects of Proanthocyanidin on the Proliferation of Sheep Follicular Granulosa Cells in vitro

YANG Xiao-feng1,2(), QIN Xiao-wei1, GUO Ze-yuan1, LV Li-hua1()   

  1. 1. College of Animal Science,Shanxi Agricultural University,Taigu 030801
    2. Department of Biology,Xinzhou Teachers University,Xinzhou 034000
  • Received:2021-07-28 Online:2022-09-26 Published:2022-10-11
  • Contact: LV Li-hua E-mail:463423663@qq.com;lihualvsxau@126.com

Abstract:

The aim of this study is to investigate the effects of proanthocyanidin(PC)on the proliferation of sheep follicular granulosa cells in vitro. Sheep follicular granulosa cells(GCs)were cultured in complete medium with different concentrations of PC(20,30,40,50,60,70 and 80 μg/mL)for 24,48 and 72 h,respectively. The proliferation rate of GCs was determined by MTT method. And the mRNA abundances of cell cycle related genes p21,p27 and cell apoptosis-related gene caspase-8 were detected by RT-qPCR. The results showed that the proliferation rate of GCs was the highest when the concentration of PC was 50 μg/mL when cultured for 48 h in vitro. And the relative mRNA abundances of three genes(p21,p27 and caspase-8)significantly decreased,respectively(P<0.01). In sum,PC could promote the proliferation of sheep follicular GCs in vitro,and which was verified at relevant mRNA level.

Key words: proanthocyanidins, granulosa cells, sheep, in vitro, proliferation