Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (6): 189-198.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1463

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SiYABBYs Involved in Rhamnoside Biosynthesis During the Flower Development of Setaria italica, Based on Metabolomics

HAN Hua-rui1(), YANG Yu-lu1, MEN Yi-han1, HAN Shang-ling1, HAN Yuan-huai1,2,3, HUO Yi-qiong1,2(), HOU Si-yu1,2,3()   

  1. 1. College of Agriculture, Shanxi Agricultural University, Taigu 030801
    2. Institute of Agricultural Bioengineer, Shanxi Agricultural University, Taigu 030801
    3. Shanxi Key Laboratory of Germplasm Innovation and Molecular Breeding in Minor Crops, Taigu 030801
  • Received:2022-11-29 Online:2023-06-26 Published:2023-07-07
  • Contact: HUO Yi-qiong, HOU Si-yu E-mail:2594488988@qq.com;yiqionghuo2013@126.com;bragren123@163.com

Abstract:

Flower organ is the premise and basis of reproductive development, and also plays a decisive role at the final stage of spikelet formation. YABBY transcription factors play an important role in the development of flower organs. In order to clarify the function of YABBY gene family and improve the yield of foxtail millet (Setaria italica), we studied the sequence characteristics and expression patterns of YABBY gene family members in the foxtail millet. We analyzed genome-wide identification of YABBY gene family in the foxtail millet, the physical and chemical properties, and tissue expression patterns of SiYABBYs gene. Also, we aligned YABBY sequences of the foxtail millet, Arabidopsis and rice(Oryza sativa)for functional prediction. The results showed that 8 YABBY family members in the foxtail millet, encoding 150-303 amino acids. And we found these SiYABBYs genes were specifically expressed in panicles at heading and pollination stages, which may be involved in the development of vegetative tissues and the formation of flower organs in the foxtail millet. The expressions of SiYABBYs were correlated with the metabolome data at heading and pollination stages of the foxtail millet. The results showed that SiYABBY1/SiYABBY5/SiYABBY7/SiYABBY8 were closely related to rhamnoside metabolic pathway. Also, the results indicated that rhamnoside accumulation in the panicles during the pollination might be related to the fertility and seed setting rate of the foxtail millet.

Key words: foxtail millet, YABBY family, metabolomics