Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (6): 105-113.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1202

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Progress in the Development and Application of RNA Reference Materials

WANG Dan1,3(), ZHAO Wen-jiang2, QI Xin3, LI Xin-ran3, LI Kai3, FAN Li1(), LI Liang3()   

  1. 1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010019
    2. Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005
    3. Institute of Quality Standardand Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2023-12-21 Online:2024-06-26 Published:2024-04-21
  • Contact: FAN Li, LI Liang E-mail:547927592@qq.com;chenlugb@126.com;liliang@caas.cn

Abstract:

RNA reference materials is an important physical standard to ensure the accuracy and consistency of nucleic acid in vitro diagnostic data. It is widely used in the detection of viral diseases. It can also be used in calibration instruments, evaluation kits and proficiency testing, which purpose is to ensure the accuracy and consistency of the measurement process and measurement results. Since 1997, WHO has developed the first hepatitis C nucleic acid international reference material for nucleic acid amplification technology, which makes the results of different laboratories and different methods in the world comparable. By 2023, there are nearly 100 kinds of RNA certified reference materials released by China on the National Sharing Platform for Reference Materials. The research and application of RNA reference materials have been paid more and more attention by metrology institutions, relevant international academic organizations and enterprises all over the world. Especially after the outbreak of the novel coronavirus epidemic, the development of RNA reference materials has become a hot spot in the development of biological reference materials. With the continuous development of the reference materials development technology, the accuracy of the reference materials has also been improved, which provides a guarantee for the accuracy and consistency of the measurement results. The preparation methods, sub-package, fixed value technology, uncertainty evaluation and application fields of RNA reference materials are briefly reviewed, which provides a reference for the development of RNA reference materials and their future applications in various fields.

Key words: RNA, reference material, characterization of reference materials, stability, homogeneity, evaluation of uncertainty