Effect of Siderophore-producing Bacteria on Peanut in the Calcareous Purple Soil

doi:10.13560/j.cnki.biotech.bull.1985.2025-0984

Abstract

Abstract:

Objective The low availability of iron (Fe) in calcareous soil leads to the prominent problem of Fe deficiency in plants. The siderophore-producing bacteria can chelate iron in the soil to promote Fe uptake of plants. Whether siderophore-producing bacteria can alleviate the problem of peanut (Arachis hypogaea L.) iron deficiency in calcareous purple soil needs to be investigated. Method Siderophore-producing bacteria were isolated using CAS (Chrome Azurol S) plates and identified by 16S rRNA gene sequencing. The optimal cultivation time for siderophore production, the acid-alkali tolerance and the capacity to secrete siderophore under acid-alkali stress of each strain were determined. Alkali-tolerant strains showing high siderophore yield were selected to inoculate around the peanut rhizosphere in a calcareous purple soil field. Effects of the siderophore-producing bacteria inoculant on peanut Fe content, photosynthesis, growth status and biomass were measured. Result Six siderophore-producing bacteria were isolated from the calcareous purple soil, and their siderophore secretion peaks and corresponding cultivation time were different. The alkali-tolerant and high siderophore-producing bacteria were Pseudomonas B3-10 and B3-2 and Cupriavidus B3-22', and their siderophore peaks respectively reached 290.2, 137.5 and 253.5 mg/L, while the concentrations dropped to 120.1, 66.4 and 86.4 mg/L in the pH 8 medium, respectively, which indicated that the alkaline stress inhibited bacterial siderophore-producing. In calcareous purple soil fields, the three strains of B3-10, B3-2 and B3-22' promoted peanut growth to varying degrees, and increased the underground Fe content, leaf photosynthesis intensity and peanut yield by 69.4%-155.3%, 9.6%-11.9% and 27.5%-52.9%, respectively. B3-10 respectively increased the Fe content in the underground part and aboveground part, and the dry weight of the underground part and aboveground part by 155.3%, 18.8%, 62.1% and 48.1%. Conclusion There are abundant siderophore-producing bacteria in the calcareous purple soil, but the alkaline condition inhibits bacteria producing siderophore. Pseudomonas B3-10 with alkali-tolerance and high siderophore-producing ability shows a significant effect on promoting Fe absorption and increasing production of peanut. It is a good strain for alleviating Fe deficiency symptoms in the calcareous soils. This study provides both theoretical support and microbial resource for enhancing Fe availability in the calcareous purple soil through applying the siderophore-producing bacteria.

Key words: siderophore-producing bacteria, iron, peanut, calcareous purple soil, growth-promoting effect

HE Qi-lu, TIAN Zhuo, DENG Jing, LI Jun-yang, YANG Wen-lin, LIU Xiang, YU Xiu-mei. Effect of Siderophore-producing Bacteria on Peanut in the Calcareous Purple Soil[J]. Biotechnology Bulletin, 2026, 42(2): 188-196.

Figures/Tables 6

Fig. 1 Phylogenetic tree of siderophore-producing bacteriaThe numbers in parentheses indicate the accession number of the sequence in GenBank

Fig. 2 Growth curves and siderophore curves of siderophore-producing bacteria

Fig. 3 Growth curves of siderophore-producing bacteria at different pH

Fig. 4 Growth curves and siderophore curves of siderophore-producing bacteria at different pH

Fig. 5 Effects of siderophore-producing bacteria on iron content and relative chlorophyll content of peanut plantsDifferent lowercase letters indicate significant differences (P<0.05)

Table 1 Effects of siderophore-producing bacteria on peanut growth

处理 Treatment	根长 Root length （cm）	株高 Plant height （cm）	地下部干重 Underground dry weight （g）	地上部干重 Aboveground dry weight （g）	产量 Yield （kg/hm²）
CK	15.67±6.66a	42.67±1.61b	0.87±0.07b	7.55±0.14b	3761.25±123.84c
B3-10	13.27±0.63a	51.23±1.31a	1.41±0.41a	11.18±1.33a	5692.25±193.12a
B3-22'	14.42±1.59a	46.08±4.63ab	1.36±0.21a	8.60±1.32b	4794.00±117.20b
B3-2	15.25±2.38a	47.08±2.43ab	1.00±0.13ab	7.49±1.31b	5750.25±101.32a

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