Screening,Identification,Condition Optimization and Application of Efficient Phosphate Solubilizing Bacteria in Sandy Fluvo Aquic Soil

doi:10.13560/j.cnki.biotech.bull.1985.2020-0550

Abstract

Abstract:

The objectives of this work are to isolate an efficient phosphate solubilizing bacterium from the sandy fluvo aquic soil,to study its effect on the change of soil phosphorus form and the growth of wheat,and to provide the theoretical basis for its application in practical production. PKO medium was used to isolate several phosphate solubilizing bacteria from sandy fluvo aquic soil,and their phosphate solubilizing abilities were compared to screen out an efficient phosphate solubilizing bacterium. The species of the bacterium was identified by combining the morphological,physiological and biochemical characteristics of the bacteria as well as 16S rDNA molecular analysis. The phosphate solubilizing conditions were optimized,and phosphorus-dissolving process was explored and growth-promoting effect of the bacterium in the soil was verified by pot experiment. As results,an efficient phosphate solubilizing bacterium W6 was screened and identified as Bacillus flexus. The results of shake flask experiment showed that the phosphorus dissolving capacity of the strain reached the maximum when the culture time was 24 hours,the initial pH was 6.0,the liquid content was 75 mL/250 mL,the carbon source was xylose,and the nitrogen source was potassium nitrate. The results from wheat pot experiment demonstrated that the contents of H₂O-P,NaHCO₃-Po,NaHCO₃-Pi,NaOH-Po,and NaOH-Pi increased by 127.3%,80.0%,54.5%,11.8%,20.0%,respectively,while the contents of hard-soluble HCl-P,concentrated HCl-Po,concentrated HCl-Pi,and concentrated H₂SO₄-P decreased by 10.9%,23.0%,42.1%,33.3%,respectively,when compared with the control. The root length,root surface area,root volume,root tip number and root diameter of wheat inoculated with the strain W6 increased 66.8%,57.0%,50.0%,13.6% and 9.1%,respectively,and the dry weight,nitrogen,phosphorus and potassium contents of wheat increased 70.5%,32.1%,24.7%,94.2% and 34.4%,respectively. In conclusion,the strain W6 may promote the transformation of phosphorus form from insoluble to effective,also promote the growth and development of wheat root system,improve the absorption and utilization efficiency of phosphorus by plants,and thus presents great application potential in agricultural production.

Key words: wheat, phosphate solubilizing bacteria, sandy fluvo aquic soil, condition optimization, phosphorus fractionation

WEI Chang, QI Xiu-xiu, WU Yue, LIU Xiao-dan, WANG Yi, JIANG Ying, LIU Hai-tao. Screening,Identification,Condition Optimization and Application of Efficient Phosphate Solubilizing Bacteria in Sandy Fluvo Aquic Soil[J]. Biotechnology Bulletin, 2021, 37(4): 85-95.

Figures/Tables 12

References 40

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项目Item	结果Results	项目Projects	结果Results
革兰氏染色 Gram staining	+	淀粉水解 Starch hydrolysis	+
好氧性实验 Aerobic test	兼性厌氧 Facultative anaerobic	柠檬酸盐利用 Citrate utilization	-
接触酶实验 Catalase test	+	硝酸盐还原 Nitrate reduction	+
甲基红（M.R）反应Methyl red reaction	-	V-P实验 V-P test	+

项目Item	结果Results	项目Projects	结果Results
革兰氏染色 Gram staining	+	淀粉水解 Starch hydrolysis	+
好氧性实验 Aerobic test	兼性厌氧 Facultative anaerobic	柠檬酸盐利用 Citrate utilization	-
接触酶实验 Catalase test	+	硝酸盐还原 Nitrate reduction	+
甲基红（M.R）反应Methyl red reaction	-	V-P实验 V-P test	+

处理Treatments		不同提取态磷素含量Contents of phosphorus in different extraction states/（g·kg^-1）								回收率 Recovery rate
处理Treatments	H₂O-P	NaHCO₃-Po	NaHCO₃-Pi	NaOH-Po	NaOH-Pi	HCl-P	浓HCl-Po Concentrated HCl-Po	浓HCl-Pi Concentrated HCl-Pi	浓H₂SO₄-P Concentrated H₂SO₄-P	回收率 Recovery rate
CK	0.11±0.02	0.35±0.10	0.22±0.00	0.17±0.02	0.15±0.03	1.56±0.07	0.74±0.07^*	0.57±0.04^*	0.09±0.02	98%
W6	0.25±0.00**	0.63±0.10^**	0.34±0.02	0.19±0.01	0.18±0.02	1.39±0.16	0.57±0.12	0.33±0.09	0.06±0.01	96%

处理Treatments		不同提取态磷素含量Contents of phosphorus in different extraction states/（g·kg^-1）								回收率 Recovery rate
处理Treatments	H₂O-P	NaHCO₃-Po	NaHCO₃-Pi	NaOH-Po	NaOH-Pi	HCl-P	浓HCl-Po Concentrated HCl-Po	浓HCl-Pi Concentrated HCl-Pi	浓H₂SO₄-P Concentrated H₂SO₄-P	回收率 Recovery rate
CK	0.11±0.02	0.35±0.10	0.22±0.00	0.17±0.02	0.15±0.03	1.56±0.07	0.74±0.07^*	0.57±0.04^*	0.09±0.02	98%
W6	0.25±0.00**	0.63±0.10^**	0.34±0.02	0.19±0.01	0.18±0.02	1.39±0.16	0.57±0.12	0.33±0.09	0.06±0.01	96%

处理Treatment	根长Root length/cm	根表面积Root surface area/cm²	根体积Root volume/cm³	根尖数Number of root shoots/个	根直径Root diameter/cm
CK	584.58±58.81	43.73±1.40	0.26±0.04	2578±71.91	0.22±0.15
W6	975.06±22.21**	68.66±1.75**	0.39±0.02**	2929±32.19*	0.24±0.02*