Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (6): 154-162.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1165

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Effects of the Polymorphism of the Seed Sequence in Porcine miR-378 on Its Function and Carcass Traits

ZHANG Ting-huan(), ZHANG Li-juan, CHEN Si-qing, GUO Zong-yi()   

  1. 1. Chongqing Academy of Animal Science,Chongqing 402460
    2. Key Laboratory of Pig Industry Sciences of Ministry of Agriculture and Rural Affairs,Chongqing 402460
  • Received:2020-09-15 Online:2021-06-26 Published:2021-07-08
  • Contact: GUO Zong-yi E-mail:ztinghuan@163.com;guozongyi@sina.com

Abstract:

The aim of this study is to explore the effects of miR-378 on its structure,function,and porcine carcass traits for the polymorphism of its seed sequence. The single nucleotide polymorphism(SNP)of Rongchang pig and Asian wild boar was used to analyze population genetic selection,to predict the changes of its structure by the polymorphism of miR-378 seed sequence,and to count the allele frequencies of miR-378 in different pig breeds. microRNA mimics were used to verify the function of miR-378 and related gene expressions in adipocytes for the polymorphism of its seed sequence. The association between the slaughter data of pig and the allele frequency of miR-378 were analyzed. The results showed that there was a strong selection signal at the miR-378 locus of Rongchang pig,and A> G mutation was found in the 5th position of miR-378-3p seed sequence. The allele frequency of this locus differed in different pig breeds,and the frequency of A allele in the pig breeds with the stronger artificial selection was higher. This mutation inhibited the function of miR-378 promoting lipid production in adipocytes,and eliminated the regulation of miR-378 on the adipocyte differentiation-related genes(PGC-1α and PGC-1 β)and the adipolysis-related genes(HSL,ATGL and CGI-58). Moreover,the genotype of this locus was significantly correlated with porcine carcass traits. Compared with the GG genotype,the thickness of backfat in AA and AG genotypes was significantly lower,and the eye-muscle area of AA genotype was significantly higher. The polymorphism of miR-378 seed sequence identified in this study could be used as a molecular marker for assisted selection of pig growth and carcass traits in pig breeding practice.

Key words: miR-378, seed sequence, polymorphism, lipogenesis, backfat thickness, eye-muscle area