Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (2): 245-251.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0975

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Research Progress in NAD+ Cap Modification at the 5' End of RNA

DONG Hai-jiao1,2(), YANG Xiao-yu3, MO Bei-xin1, CHEN Xue-mei4, CUI Jie1()   

  1. 1. College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518037
    2. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering,Shenzhen University,Shenzhen 518060
    3. College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018
    4. Department of Botany and Plant Sciences,Institute of Integrative Genome Biology,University of California,Riverside,CA,92521,USA
  • Received:2021-07-30 Online:2022-02-26 Published:2022-03-09
  • Contact: CUI Jie E-mail:547860420@qq.com;huademunaiyi@163.com

Abstract:

The m7G cap,which has long been considered as a hallmark of eukaryotic mRNAs,protects RNAs from degradation and recruits the machinery for splicing,polyadenylation,nuclear export,and translation. The nucleotide-containing metabolite,nicotinamide adenine dinucleotide(NAD+),has recently been detected at the 5' end of RNAs in several species including prokaryotes as a new cap. Now research on the biological functions of NAD+-capped RNAs(NAD-RNAs)is still at the initial stage. This review summarizes the discovery of NAD-RNAs as well as the development of its detection and identification technology,discusses the regulatory functions of the NAD+ cap on RNA as well as the factors affecting RNA NAD+ capping and decapping,and speculates on the potential functions of NAD-RNAs in growth,development and environmental responses. Finally,we propose future research directions and topics in order to garner attention on the outstanding questions regarding the molecular and biological functions NAD-RNAs.

Key words: RNA cap, NAD+, NAD-RNA, post-transcriptional regulation, biological function