Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (11): 70-79.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0193
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CHEN Yu-jie(), ZHENG Hua-bao, ZHOU Xin-yan()
Received:
2022-02-17
Online:
2022-11-26
Published:
2022-12-01
Contact:
ZHOU Xin-yan
E-mail:201818040216@stu.zafu.edu.cn;20200087@zafu.edu.cn
CHEN Yu-jie, ZHENG Hua-bao, ZHOU Xin-yan. Modified High-throughput Sequencing Reveals the Effects of Different Algicides towards Algal Community[J]. Biotechnology Bulletin, 2022, 38(11): 70-79.
氨氮Ammonia nitrogen/(mg·L-1) | 总氮Total nitrogen/(mg·L-1) | 总磷Total phosphorus/(mg·L-1) | 化学需氧量Chemical oxidation demand/(mg·L-1) | 浊度 Turbidity/(mg·L-1) | 藻细胞密度 Algal cell density/(106 cell·mL-1) | 叶绿素a Chlorophyll a/(μg·L-1) |
---|---|---|---|---|---|---|
0.60 | 0.79 | 0.05 | 35 | 23.7±1.1 | 1.41 | 184 |
Table 1 Basic water quality parameters of the landscape pond
氨氮Ammonia nitrogen/(mg·L-1) | 总氮Total nitrogen/(mg·L-1) | 总磷Total phosphorus/(mg·L-1) | 化学需氧量Chemical oxidation demand/(mg·L-1) | 浊度 Turbidity/(mg·L-1) | 藻细胞密度 Algal cell density/(106 cell·mL-1) | 叶绿素a Chlorophyll a/(μg·L-1) |
---|---|---|---|---|---|---|
0.60 | 0.79 | 0.05 | 35 | 23.7±1.1 | 1.41 | 184 |
Fig.1 Removal efficiencies of algal cells by 3 algicides Data of the bar marked by different letters indicate significant difference at 0.05 levels by Tukey’s test
Fig. 2 Influences of 3 algicides on algal community compositions(phylum level) “Initial” refers to the initial algal community;“CK” refers to control check;“Cl”,“Cu” and “Bio” refers to Ca(ClO)2,CuSO4 and bio-algicide,respectively;number indicates the dosage of algicide(mg/L);NPMA refers to the samples without PMA pre-treatment;PMA refers to the samples with PMA pre-treatment,the same below
物种名称 Species name | Initial /% | CKNPMA /% | Cl-0.5NPMA /% | Cl-5.0NPMA /% | Cu-0.1NPMA /% | Cu-1.0NPMA /% | Bio-10NPMA /% | Bio-50NPMA /% | CKPMA /% | Cl-0.5PMA /% | Cl-5.0PMA /% | Cu-0.1PMA /% | Cu-1.0PMA /% | Bio-10PMA /% | Bio-50PMA /% |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Leptolyngbya boryana* | 0.27 | 0.44 | 0.33 | 0.49 | 0.22 | 0.23 | 0.50 | 0.87 | 20.60 | 28.01 | 28.67 | 41.52 | 18.18 | 27.85 | 28.01 |
Chamaesiphon minutus* | <0.01 | 0.02 | <0.01 | 0.03 | <0.01 | 0.01 | <0.01 | 0.02 | 0.29 | 0.62 | 0.56 | 0.66 | 0.79 | 0.65 | 1.51 |
Nannochloris normandinae** | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | 0.047 | 0.33 | 0.73 | 0.43 | 0.27 | 0.12 | 0.41 |
Xylochloris irregularis** | 0.02 | 0.02 | 0.02 | 0.04 | <0.01 | 0.01 | <0.01 | <0.01 | 0.07 | 0.07 | 0.61 | 0.31 | 0.20 | 0.24 | 0.27 |
Pectinodesmus pectinatus** | <0.01 | 0.06 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | 0.14 | 0.25 | 0.11 | <0.01 | 0.46 | 0.18 | 0.44 |
Lobosphaera incisa** | 0.57 | 0.02 | 0.02 | 0.18 | <0.01 | 0.08 | <0.01 | 0.18 | 0.03 | 0.03 | 0.16 | 0.18 | <0.01 | 0.03 | 0.18 |
Dinophysis acuta*** | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | 0.56 | <0.01 | <0.01 |
Pleurocapsa sp. 04302-00001* | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | 0.57 | <0.01 | <0.01 | <0.01 |
Tetradesmus obliquus** | 0.04 | 0.05 | 0.02 | 0.04 | 0.02 | 0.01 | 0.04 | 0.06 | 0.01 | <0.01 | <0.01 | 0.01 | <0.01 | <0.01 | 0.10 |
Neochloris aquatica** | <0.01 | 0.02 | 0.03 | 0.05 | <0.01 | 0.02 | 0.02 | 0.03 | 0.05 | <0.01 | 0.03 | <0.01 | <0.01 | 0.02 | <0.01 |
Table 2 Algal community compositions in species level under 3 algicides treatment(species with top 10 relative abundance)
物种名称 Species name | Initial /% | CKNPMA /% | Cl-0.5NPMA /% | Cl-5.0NPMA /% | Cu-0.1NPMA /% | Cu-1.0NPMA /% | Bio-10NPMA /% | Bio-50NPMA /% | CKPMA /% | Cl-0.5PMA /% | Cl-5.0PMA /% | Cu-0.1PMA /% | Cu-1.0PMA /% | Bio-10PMA /% | Bio-50PMA /% |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Leptolyngbya boryana* | 0.27 | 0.44 | 0.33 | 0.49 | 0.22 | 0.23 | 0.50 | 0.87 | 20.60 | 28.01 | 28.67 | 41.52 | 18.18 | 27.85 | 28.01 |
Chamaesiphon minutus* | <0.01 | 0.02 | <0.01 | 0.03 | <0.01 | 0.01 | <0.01 | 0.02 | 0.29 | 0.62 | 0.56 | 0.66 | 0.79 | 0.65 | 1.51 |
Nannochloris normandinae** | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | 0.047 | 0.33 | 0.73 | 0.43 | 0.27 | 0.12 | 0.41 |
Xylochloris irregularis** | 0.02 | 0.02 | 0.02 | 0.04 | <0.01 | 0.01 | <0.01 | <0.01 | 0.07 | 0.07 | 0.61 | 0.31 | 0.20 | 0.24 | 0.27 |
Pectinodesmus pectinatus** | <0.01 | 0.06 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | 0.14 | 0.25 | 0.11 | <0.01 | 0.46 | 0.18 | 0.44 |
Lobosphaera incisa** | 0.57 | 0.02 | 0.02 | 0.18 | <0.01 | 0.08 | <0.01 | 0.18 | 0.03 | 0.03 | 0.16 | 0.18 | <0.01 | 0.03 | 0.18 |
Dinophysis acuta*** | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | 0.56 | <0.01 | <0.01 |
Pleurocapsa sp. 04302-00001* | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | 0.57 | <0.01 | <0.01 | <0.01 |
Tetradesmus obliquus** | 0.04 | 0.05 | 0.02 | 0.04 | 0.02 | 0.01 | 0.04 | 0.06 | 0.01 | <0.01 | <0.01 | 0.01 | <0.01 | <0.01 | 0.10 |
Neochloris aquatica** | <0.01 | 0.02 | 0.03 | 0.05 | <0.01 | 0.02 | 0.02 | 0.03 | 0.05 | <0.01 | 0.03 | <0.01 | <0.01 | 0.02 | <0.01 |
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