Comparison and Analysis of Endophytic Bacterial Communities in Different Perennial Rice Varieties

doi:10.13560/j.cnki.biotech.bull.1985.2023-1088

Abstract

Abstract:

【Objective】To explore the structure and potential functions of endophytic bacterial microbiota in perennial rice, as well as the relationship between endophytic bacteria in perennial rice and host agronomic traits. 【Method】The diversity, composition and metabolic function of endophytic bacteria in different in perennial rice varieties（PR23, PR25, and PR107）were characterized via Illumina sequencing of the 16S rRNA gene. 【Result】These results showed that the beta diversity of endophytic bacterial communities significantly differed among the distinct genotypes of perennial rice. Due to the differentiation of tissue structures, perennial rice varieties shaped distinctive ecological niches for endophytes in different tissue compartments. Proteobacteria, Firmicutes and Bacteroidetes were the dominant endophytic bacterial phyla in the underground tissues of perennial rice, while Proteobacteria, Actinobacteria and Firmicutes were the dominant phyla in the aboveground tissues. These perennial rice varieties had different endophytic bacterial taxa with specific amplicon sequence variants（ASVs）, dominant genera and specific biomarkers. The endophytic bacterial communities of perennial rice were reassembled for various metabolic functions, including biosynthesis, degradation/utilization/assimilation, detoxification, generation of precursor metabolite and energy, glycan pathways, macromolecule modification and metabolic clusters. Significantly different MetaCyc pathways of endophytic bacteria in perennial rice varieties with distinct genotypes were involved in the biosynthesis and degradation of esters, alcohols and polysaccharides. 【Conclusion】These results indicated host genotypes and tissues differentiation affect the community structure, species composition and metabolic function of endophytic bacteria in perennial rice.

Key words: perennial rice, endophytic bacteria, community structure, 16S rRNA sequencing

KONG De-ting, QI Xiao-han, LIU Xing-lei, LI Li-ping, HU Feng-yi, HUANG Li-yu, QIN Shi-wen. Comparison and Analysis of Endophytic Bacterial Communities in Different Perennial Rice Varieties[J]. Biotechnology Bulletin, 2024, 40(5): 225-236.

Figures/Tables 5

Fig. 1 α and β diversity of endophytic bacterial communities in the aboveground and the underground compartments of perennial rice varieties PR23 and PR25 are japonica subspecies, and PR107 is indica subspecies

Fig. 2 Composition of endophytic bacteria at phyla level in the aboveground and the underground compartments（A）of perennial rice varieties as well as the common and variety-specific endophytic bacterial ASVs in perennial rice（B）

Fig. 3 Endophytic bacterial genera（A）and biomarking endophytic bacteria（B）with top 20 of relative abundances in the aboveground and the underground compartments of perennial rice varieties

Fig. 4 MetaCyc pathways of endophytic bacteria in the aboveground and the background compartments of perennial rice varieties

Fig. 5 Significantly differential MetaCyc pathways enriched in the aboveground and the belowground compartments of perennial rice

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