Study on the Microbial Community of Rhizosphere Soil in Ancient Tea Garden and Modern Organic Tea Garden in Jingmai Mountain

doi:10.13560/j.cnki.biotech.bull.1985.2023-1104

Abstract

Abstract:

【Objective】It is to study the composition and diversity of rhizosphere soil microbial communities in tea gardens of different habitats, to identify the main driving factors affecting rhizosphere soil microbial communities, and to provide theoretical basis for the study of the relationship between rhizosphere soil microbial communities and pest control of tea trees, as well as for the function and utilization of soil microbials. 【Method】We used Illumina-MiSeq high-throughput sequencing technology to investigate the community composition and diversity of soil microorganisms in the rhizosphere soil communities of Jingmai Mountain Ancient Tea Garden（GS）and Jingmai Mountain Modern Organic Tea Garden（TS）, and used redundancy analysis to explore the relationship between soil nutrients and microorganisms.【Result】The cation exchange capacity, organic matter, humus, ammonia nitrogen, ammonium nitrogen, available phosphorus, and water content in the rhizosphere soil in TS were higher than those in the GS, showing significant differences; the dominant bacterial populations in both gardens were Actinobacteria, Acidobacteria, and Planctomycetes, and the dominant fungal populations are Ascomycota, Mortierellomycota, and Basidiomycota, except for the unclassified genera. The dominant bacterial genera in GS are very similar to those in TS, but the relative abundance of each genus varies greatly in two gardens. Redundancy analysis shows that cation exchange capacity, organic matter, humus, ammonia nitrogen, and ammonium nitrogen were the main environmental factors causing differences in the microbial community structure and diversity in the rhizosphere soil of two tea gardens. 【Conclusion】The nutrient and species richness of the rhizosphere soil of TS was significantly higher than that of Gs, and the composition of the dominant bacterial genera in the rhizosphere soil of two tea gardens was highly similar, but their abundance varied significantly, showing a high degree of correlation with soil nutrients.

Key words: Jingmai Mountain, Pu-erh tea, ancient tea garden, rhizosphere microorganisms, high-throughput sequencing, community structure

YU Li-jun, WANG Qiao-mei, PENG Wen-shu, YAN Liang, YANG Rui-juan. Study on the Microbial Community of Rhizosphere Soil in Ancient Tea Garden and Modern Organic Tea Garden in Jingmai Mountain[J]. Biotechnology Bulletin, 2024, 40(5): 237-247.

Figures/Tables 10

References 27

[1]	杨瑞娟, 王桥美, 彭文书, 等. 云南景迈山不同生境茶园根际土壤微生物群落多样性初步研究[J]. 热带作物学报, 2021, 42(4): 1182-1189. doi: 10.3969/j.issn.1000-2561.2021.04.039
	Yang RJ, Wang QM, Peng WS, et al. A preliminary study on the distribution diversity of rhizosphere soil microbial community in tea plantations with different habitats of jingmai mountain, Yunnan, China[J]. Chin J Trop Crops, 2021, 42(4): 1182-1189.
[2]	Li YC, Li Z, Li ZW, et al. Variations of rhizosphere bacterial communities in tea(Camellia sinensis L.) continuous cropping soil by high-throughput pyrosequencing approach[J]. J Appl Microbiol, 2016, 121(3): 787-799. doi: 10.1111/jam.13225 pmid: 27377624
[3]	俞慎, 何振立, 陈国潮, 等. 不同树龄茶树根层土壤化学特性及其对微生物区系和数量的影响[J]. 土壤学报, 2003, 40(3): 433-439.
	Yu S, He ZL, Chen GC, et al. Soil chemical characteristics and their impacts on soil microflora in the root layer of tea plants with different cultivating ages[J]. Acta Pedol Sin, 2003, 40(3): 433-439.
[4]	浦滇, 罗义菊, 陈洪宇, 等. 长期种植云南大叶种茶对土壤真菌多样性的影响[J]. 应用与环境生物学报, 2023, 29(2): 440-448.
	Pu D, Luo YJ, Chen HY, et al. Effects of long-term cultivation of Yunnan large-leaf tea(Camellia sinensis var. assamica)on soil fungal community characteristics[J]. Chin J Appl Environ Biol, 2023, 29(2): 440-448.
[5]	张旭博, 徐梦, 史飞. 藏东南林芝地区典型农业土地利用方式对土壤微生物群落特征的影响[J]. 农业环境科学学报, 2020, 39(2): 331-342.
	Zhang XB, Xu M, Shi F. Impact of typical agricultural land use on the characteristics of soil microbial communities in the Nyingchi region of southeastern Tibet[J]. J Agro Environ Sci, 2020, 39(2): 331-342.
[6]	刘会梅, 张天宇. 土壤真菌研究进展[J]. 山东农业大学学报: 自然科学版, 2008, 39(2): 326-330.
	Liu HM, Zhang TY. Research progress on soil fungi[J]. J Shandong Agric Univ Nat Sci Ed, 2008, 39(2): 326-330.
[7]	Jackson LE, Bowles TM, Hodson AK, et al. Soil microbial-root and microbial-rhizosphere processes to increase nitrogen availability and retention in agroecosystems[J]. Curr Opin Environ Sustain, 2012, 4(5): 517-522.
[8]	Huang Q, Jiao F, Huang YM, et al. Response of soil fungal community composition and functions on the alteration of precipitation in the grassland of Loess Plateau[J]. Sci Total Environ, 2021, 751: 142273.
[9]	Boddington CL, Dodd JC. The effect of agricultural practices on the development of indigenous arbuscular mycorrhizal fungi. I. Field studies in an Indonesian ultisol[J]. Plant Soil, 2000, 218(1): 137-144.
[10]	卢开阳. 云南11个茶山的大叶种茶树根际土壤微生物遗传多样性研究[D]. 昆明: 云南师范大学, 2016.
	Lu KY. Study on microbial genetic diversity in large leaf tea rhizosphere soil at 11 mountains from Yunnan province[D]. Kunming: Yunnan Normal University, 2016.
[11]	Sarkar S, Seenivasan S, Asir RPS. Biodegradation of propargite by Pseudomonas putida, isolated from tea rhizosphere[J]. J Hazard Mater, 2010, 174(1/2/3): 295-298.
[12]	黄芳芳, 李勤, 黄建安. 茶树根际微生物研究进展[J]. 茶叶科学, 2020, 40(6): 715-723.
	Huang FF, Li Q, Huang JA. Research progress of tea rhizosphere microorganisms[J]. J Tea Sci, 2020, 40(6): 715-723.
[13]	Sharma S, Chen C, Navathe S, et al. A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection[J]. Sci Rep, 2019, 9(1): 4054. doi: 10.1038/s41598-019-40930-x pmid: 30858512
[14]	陈丽莹, 张玉满, 陈晓英, 等. 茶树叶际选择性富集的内生细菌的鉴定[J]. 微生物学报, 2018, 58(10): 1776-1785.
	Chen LY, Zhang YM, Chen XY, et al. Identification of endophytic bacteria selectively enriched in Camellia sinensis leaf[J]. Acta Microbiol Sin, 2018, 58(10): 1776-1785.
[15]	夏丽飞, 梁名志, 王丽, 等. 勐海晒青茶品质化学研究[J]. 中国农学通报, 2012, 28(16): 239-244.
	Xia LF, Liang MZ, Wang L, et al. Studying on the quality of Menghai's sunny dried tea[J]. Chin Agric Sci Bull, 2012, 28(16): 239-244.
[16]	Jiang PK, Xu QF, Xu ZH, et al. Seasonal changes in soil labile organic carbon pools within a Phyllostachys praecox stand under high rate fertilization and winter mulch in subtropical China[J]. For Ecol Manag, 2006, 236(1): 30-36.
[17]	曾美娟, 钟永嘉, 刁勇. 药用植物根际促生菌促生机理研究进展[J]. 生物技术通报, 2017, 33(11): 13-18. doi: 10.13560/j.cnki.biotech.bull.1985.2017-0359
	Zeng MJ, Zhong YJ, Diao Y. Promoting mechanism of plant growth-promoting rhizobacteria in medicinal plants[J]. Biotechnol Bull, 2017, 33(11): 13-18.
[18]	Osorio NW, Habte M. Soil phosphate desorption induced by a phosphate-solubilizing fungus[J]. Commun Soil Sci Plant Anal, 2014, 45(4): 451-460.
[19]	Tamayo-Vélez Á, Osorio NW. Soil fertility improvement by litter decomposition and inoculation with the fungus Mortierella sp. in avocado plantations of Colombia[J]. Commun Soil Sci Plant Anal, 2018, 49(2): 139-147.
[20]	孙建光, 胡海燕, 刘君, 等. 农田环境中固氮菌的促生潜能与分布特点[J]. 中国农业科学, 2012, 45(8): 1532-1544. doi: 10.3864/j.issn.0578-1752.2012.08.009
	Sun JG, Hu HY, Liu J, et al. Growth promotion potential and distribution features of nitrogen-fixing bacteria in field environments[J]. Sci Agric Sin, 2012, 45(8): 1532-1544.
[21]	Azarias Guimarães A, Duque Jaramillo PM, Simão Abrahão Nóbrega R, et al. Genetic and symbiotic diversity of nitrogen-fixing bacteria isolated from agricultural soils in the western Amazon by using cowpea as the trap plant[J]. Appl Environ Microbiol, 2012, 78(18): 6726-6733.
[22]	梁晓洁, 刘志华, 张平, 等. 抗油桐枯萎病木霉菌的分离鉴定及抑菌作用研究[J]. 菌物学报, 2020, 39(5): 795-805. doi: 10.13346/j.mycosystema.190392
	Liang XJ, Liu ZH, Zhang P, et al. Trichoderma spp. isolated from rhizosphere soil of wilt-resistant Vernicia fordii and their inhibition effects against Fusarium oxysporum[J]. Mycosystema, 2020, 39(5): 795-805.
[23]	汪立群, 颜小梅, 郭小双, 等. 紫娟、云抗10号两个茶树品种内生菌多样性研究[J]. 安徽农业大学学报, 2016, 43(1): 1-5.
	Wang LQ, Yan XM, Guo XS, et al. Diversity of endophytic microorganisms zijuan and Yunkang 10 of Camellia sinensis[J]. J Anhui Agric Univ, 2016, 43(1): 1-5.
[24]	杨瑞娟, 王桥美, 龚婉莹, 等. 云南景迈山不同生境茶园及茶窖空气微生物群落分布多样性研究[J]. 云南农业大学学报: 自然科学, 2020, 35(4): 659-666.
	Yang RJ, Wang QM, Gong WY, et al. Distribution diversity of microbial communities in the air of tea gardens and tea cellars in different habitats of jingmai mountain, Yunnan Province[J]. J Yunnan Agric Univ Nat Sci, 2020, 35(4): 659-666.
[25]	张英英, 魏玉杰, 吴之涛, 等. 不同种植年限对特殊药材土壤化学性质和微生物多样性的影响[J]. 干旱地区农业研究, 2023, 41(1): 150-159.
	Zhang YY, Wei YJ, Wu ZT, et al. Effects of different cropping years on soil chemical properties of special medicine source plant[J]. Agric Res Arid Areas, 2023, 41(1): 150-159.
[26]	李丽艳, 朱瑞艳, 杜迎辉, 等. 微生物肥料对草莓根腐病防治效果及对根围土壤微生物群落多样性的影响[J]. 安徽农业科学, 2018, 46(33): 111-113.
	Li LY, Zhu RY, Du YH, et al. Effect of microbial fertilizer on the control of strawberry root rot and the diversity of soil microbial communities[J]. J Anhui Agric Sci, 2018, 46(33): 111-113.
[27]	黄昆鹏, 董昆乐, 李芳芳, 等. 烟草抗病嫁接对根际土壤微生物多样性的影响[J]. 江苏农业科学, 2023, 51(20): 239-247.
	Huang KP, Dong KL, Li FF, et al. Impacts of disease-resistant grafting on microbial diversity in rhizosphere soil of tobacco[J]. Jiangsu Agric Sci, 2023, 51(20): 239-247.

土壤养分Soil nutrients	GS	TS
阳离子交换量Cation exchange capacity/（mol·kg^-1）	22.50±0.69a	24.60±0.98a
有机质Soil organic matter /（g·kg^-1）	83.60±0.98a	106.60±0.06b
腐殖质Soil humus /（g·kg^-1）	43.00±0.46a	54.40±0.46b
氨氮Ammonia nitrogen /（mg·kg^-1）	418.90±3.00a	513.00±1.33b
铵态氮Ammonium nitrogen /（mg·kg^-1）	508.70±1.21a	662.90±0.52b
有效磷Available phosphorous /（mg·kg^-1）	0.90±0.02a	66.40±0.52b
速效钾Available K /（mg·kg^-1）	61.00±0.17a	61.00±0.69a
水分Moisture /%	2.50±0.02a	2.90±0.08b

土壤养分Soil nutrients	GS	TS
阳离子交换量Cation exchange capacity/（mol·kg^-1）	22.50±0.69a	24.60±0.98a
有机质Soil organic matter /（g·kg^-1）	83.60±0.98a	106.60±0.06b
腐殖质Soil humus /（g·kg^-1）	43.00±0.46a	54.40±0.46b
氨氮Ammonia nitrogen /（mg·kg^-1）	418.90±3.00a	513.00±1.33b
铵态氮Ammonium nitrogen /（mg·kg^-1）	508.70±1.21a	662.90±0.52b
有效磷Available phosphorous /（mg·kg^-1）	0.90±0.02a	66.40±0.52b
速效钾Available K /（mg·kg^-1）	61.00±0.17a	61.00±0.69a
水分Moisture /%	2.50±0.02a	2.90±0.08b

样品代号Sample	丰富度指数Richness index				多样性指数Diversity index
	ACE index		Chao 1 index		Simpson index		Shannon index
	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi
GS	800.63±34.61a	259.06±25.78a	806.52±32.86a	261.17±27.19a	0.10±0.03a	0.07±0.05a	4.20±0.33a	3.99±0.61a
TS	886.57±22.31a	295.12±21.03a	892.61±23.77a	298.08±20.68a	0.02±0.00b	0.09±0.02a	5.28±0.03b	3.43±0.12a

样品代号Sample	丰富度指数Richness index				多样性指数Diversity index
	ACE index		Chao 1 index		Simpson index		Shannon index
	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi
GS	800.63±34.61a	259.06±25.78a	806.52±32.86a	261.17±27.19a	0.10±0.03a	0.07±0.05a	4.20±0.33a	3.99±0.61a
TS	886.57±22.31a	295.12±21.03a	892.61±23.77a	298.08±20.68a	0.02±0.00b	0.09±0.02a	5.28±0.03b	3.43±0.12a

门Phylum	科Family	属Genus	OUT	GS/%	TS/%
Actinobacteria	Micrococcaceae	Arthrobacter	OTU1	30.08	8.54
Acidobacteria	uncultured_bacterium_o_Acidobacteriales	uncultured_bacterium_o_Acidobacteriales	OTU6	-	1.89
			OTU13	-	1.79
			OTU19	-	1.39
	Solibacteraceae_Subgroup_3	Candidatus_Solibacter	OTU37	-	1.45
			OTU174	-	1.37
			OTU15	-	1.14
		Bryobacter	OTU34	-	1.00
	uncultured_bacterium_o_Subgroup_2	uncultured_bacterium_o_Subgroup_2	OTU9	-	1.20
		uncultured_bacterium_o_Subgroup_7	OTU16	1.13	-
	Streptomycetaceae	uncultured_bacterium_f_Streptomycetaceae	OTU8	-	1.22
Bacteroidetes	Prevotellaceae	Prevotella_7	OTU24	1.07	-
Chloroflexi	uncultured_bacterium_c_AD3	uncultured_bacterium_c_AD3	OTU3	7.49	1.68
			OTU135	2.53	-
Proteobacteria	Xanthobacteraceae	uncultured_bacterium_f_Xanthobacteraceae	OTU7	3.25	4.13
			OTU4	-	1.63
			OTU11	1.31	-
		Bradyrhizobium	OTU2	2.17	4.81
	Unclassified	Acidibacter	OTU31	-	1.62
	Polyangiaceae	Pajaroellobacter	OTU28	-	1.27
	Unclassified	Acidibacter	OTU31	1.26	-