转录组测序技术的研究和应用进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-0374

摘要/Abstract

摘要： 转录组（Transcriptome）是指特定细胞或组织中全部转录产物,包括信使RNA,核糖体RNA、转运RNA以及非编码RNA。高通量测序技术的快速发展,为从整体水平系统地研究转录组学研究提供快捷可靠的平台。综述了当前主流的高通量测序技术及其在转录组学研究中的应用,并讨论了转录组数据分析中一些值得关注的问题,以及转录组测序技术在生物学研究中的应用方向。

关键词: 转录组, RNA-seq, 二代测序, 三代测序, 单细胞转录组

Abstract: The transcriptome is the set of all RNA molecules in the specific tissue or cell,including message RNA,ribosome RNA,transfer RNA and non-coding RNAs. The rapid development of high-throughput sequencing technology provides a fast and reliable platform for the systematic study of transcriptome. Here we reviewed the current high-throughput sequencing technology and its application in transcriptome research,and discussed some issues worthy of attention in transcriptome data analysis,as well as the application direction of transcriptome sequencing technology in biological research.

Key words: transcriptome, RNA-Seq, next generation sequencing, third-generation sequencing, single-cellRNA-seq

崔凯, 吴伟伟, 刁其玉. 转录组测序技术的研究和应用进展[J]. 生物技术通报, 2019, 35(7): 1-9.

CUI Kai, WU Wei-wei, DIAO Qi-yu. Application and Research Progress on Transcriptomics[J]. Biotechnology Bulletin, 2019, 35(7): 1-9.

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