合成生物学在天然产物研究中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2017.01.004

摘要/Abstract

摘要： 天然产物作为临床用药的重要组成部分和新药发现的重要来源，已经成为医药领域不可或缺的成员之一。但是传统的天然产物筛选方法在一定程度上制约了新型天然产物的开发，鉴别与改造天然产物的生物合成路径为全新天然产物的发现提供了思路。近年来，生物信息学和合成生物学技术的蓬勃发展为天然产物的探索与改造带来了新的曙光。归纳总结了合成生物学技术相关的基因重组策略和基因簇调控方法，并讨论了其在天然产物研究中的应用及存在的问题。

关键词: 天然产物, 合成生物学, 基因重组, 基因编辑, 底盘细胞设计, 异源表达, CRISPR/Cas9

Abstract: As an important source of clinical medicine and new drug candidate,natural product plays an essential role in therapeutic and pharmaceutical industry. However,traditional methods for natural product discovery constrain the development of new compounds at certain extent,and identifying and refactoring the biosynthetic pathways of natural products provided new thoughts for the discovery of new natural product. Currently,with the prosperous development of bioinformatics and synthetic biology technologies,a new era of natural product discovery and engineering can be foreseen. Here,we summarize recent advances on the strategies of genetic recombination and gene cluster regulation related to synthetic biology techniques,and discuss their applications and issues in studying natural products.

Key words: natural product, synthetic biology, genetic recombination, gene editing, chassis design, heterologous expression, CRISPR/Cas9

王丽苹，罗云孜. 合成生物学在天然产物研究中的应用[J]. 生物技术通报, 2017, 33(1): 35-47.

WANG Li-ping, LUO Yun-zi. Applications of Synthetic Biology in the Research of Natural Product[J]. Biotechnology Bulletin, 2017, 33(1): 35-47.

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