动物转基因高效表达策略研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-0989

摘要/Abstract

摘要： 外源基因在动物体内、细胞内表达时,常由于各种原因导致基因沉默。目前,主要有两种策略用于打破该瓶颈：一是利用友好位点;二是优化外源基因顺式作用元件。利用友好位点就是通过定点整合技术将外源基因插入至活性转录区域;优化外源基因顺式作用元件即采用合适的启动子、增强子、内含子、染色质开放元件、核基质附着区等元件,构建出高效表达载体。对相关策略进行概述,为动物基因工程育种提供参考。

关键词: 转基因动物, 外源基因, 友好位点, 基因编辑

Abstract: The exogenous genes are often silenced in transgenic animals or cells due to a variety of reasons. Currently,there are two main strategies to break this bottleneck：one is to use safe harbors;the second is to optimize cis-acting elements of exogenous genes. Using safe harbors is to insert exogenous genes into the active transcription region via site-specific integration technologies. Optimizing cis-acting elements is to select the appropriate promoters,enhancers,introns,ubiquitous chromatin opening elements（UCOE）,matrix attachment regions（MARs）to construct efficient expression vector. This review summarizes the relevant strategies for improving the expression efficiency of exogenous genes and provides a reference for genetic engineering animal breeding.

Key words: transgenic animals, exogenous genes, safe harbors, gene editing

赵旭东, 黄永志, 毕延震, 董发明. 动物转基因高效表达策略研究进展[J]. 生物技术通报, 2020, 36(3): 45-53.

ZHAO Xu-dong, HUANG Yong-zhi, BI Yan-zhen, DONG Fa-ming. Strategies for Efficient Exogenous Gene Expression in Transgenic Animals[J]. Biotechnology Bulletin, 2020, 36(3): 45-53.

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