低温胁迫对柳杉不同无性系的影响及抗寒性评价

doi:10.13560/j.cnki.biotech.bull.1985.2021-0615

摘要/Abstract

摘要：

通过分析柳杉不同无性系抗寒性的差异,评价柳杉不同无性系抗寒能力,为柳杉的引种栽培及抗寒育种提供理论依据。以生长一致的10个柳杉无性系的2年生枝条为试验材料,分别在4℃、0℃、-4℃、-8℃、-12℃、-16℃和-20℃目标低温下处理12 h,对其叶片的丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性及叶绿素荧光参数进行测定,结合主成分分析及隶属函数计算对测定的指标进行综合评价和抗寒性排序。结果表明,低温胁迫下随着温度逐渐降低,MDA含量在一定温度范围内具有一定的波动性,但总体上呈现先上升后下降再上升的趋势;SOD活性在低温初期先出现短暂的下降后经抗寒锻炼呈现出上升又下降的趋势;柳杉不同无性系PS II最大光化学效率（Fv/Fm）和PS II的实际光合量子产量（Y（II））表现出相似的变化趋势,其值在0℃达到最高,0℃之后持续降低;光化学淬灭系数（qP）在低温初期无明显变化,0℃后随着低温胁迫的加剧而降低。柳杉不同无性系抗寒性具有显著差异,其中57#、68#和32#无性系抗寒性较强,3#、25#和42#无性系抗寒性较弱。

关键词: 柳杉, 低温胁迫, 生理指标, 主成分分析, 抗寒性评价

Abstract:

This study is aimed to assess the cold resistance of Cryptomeria fortunei by analyzing the difference of cold resistance among its different clones,providing theoretical basis for the introduction,cultivation and cold resistance breeding of C. fortunei. The study subjects were 10 2-year branches from different clones,which were treated at 4℃,0℃,-4℃,-8℃,-12℃,-16℃ and -20℃ for 12 h,respectively. They were then measured in terms of the contents of malondialdehyde（MDA）,the activity of superoxide dismutase（SOD）and the parameters of chlorophyll fluorescence. Combined with principal component analysis and membership function calculation,comprehensive evaluation and cold resistance ranking were carried out. The results showed that：as the temperature decreased,the content of MDA fluctuated within a certain range of temperature,but generally it exhibited a tendency to increase first,then decrease,and finally increase. In contrast,the activity of SOD decreased shortly at first,and then increased and finally decreased after cold acclimation. The maximum photochemical efficiency（Fv/Fm）and the actual photosynthetic quantum yield（Y（II））of PS II in the different clones presented a similar trend,reaching their peak at 0℃ and decreased as the temperature futher decreasing. The photochemical quenching coefficient（qP）did not change significantly at the beginning of cold stress,but it decreased as the low temperature stress aggravated to below 0℃. There were significant differences in cold resistance among different clones of C. fortunei,among which 57#,68# and 32# clones were of stronger cold resistance,while 3#,25# and 42# clones were of weaker cold resistance.

Key words: Cryptomeria fortunei, low temperature stress, physiological index, principal component analysis, cold resistance evaluation

崔洁冰, 张萌, 张莹婷, 徐进. 低温胁迫对柳杉不同无性系的影响及抗寒性评价[J]. 生物技术通报, 2022, 38(3): 31-40.

CUI Jie-bing, ZHANG Meng, ZHANG Ying-ting, XU Jin. Effects of Low Temperature Stress on Different Clones of Cryptomeria fortunei and Evaluation of Their Cold Resistance[J]. Biotechnology Bulletin, 2022, 38(3): 31-40.

图/表 10

参考文献 41

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无性系 Clones	来源地 Origin	无性系 Clones	来源地 Origin
3#	福建省仙游县溪口林场 Xikou forest farm,Xianyou county,Fujian province	57#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian province
25#	浙江省临安区天目山林场 Tianmushan forest farm,Lin'an county,Zhejiang Province	66#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian province
32#	浙江省临安区天目山林场 Tianmushan forest farm,Lin'an county,Zhejiang Province	68#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian province
42#	浙江省文成县石垟林场 Shilong forest farm,Wencheng county,Zhejiang Province	74#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian province
54#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian Province	X1#	南京林业大学校园 Nanjing Forestry University campus

无性系 Clones	来源地 Origin	无性系 Clones	来源地 Origin
3#	福建省仙游县溪口林场 Xikou forest farm,Xianyou county,Fujian province	57#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian province
25#	浙江省临安区天目山林场 Tianmushan forest farm,Lin'an county,Zhejiang Province	66#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian province
32#	浙江省临安区天目山林场 Tianmushan forest farm,Lin'an county,Zhejiang Province	68#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian province
42#	浙江省文成县石垟林场 Shilong forest farm,Wencheng county,Zhejiang Province	74#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian province
54#	福建省霞浦县杨梅岭林场 Yangmeiling forest farm,Xiapu county,Fujian Province	X1#	南京林业大学校园 Nanjing Forestry University campus

主成分 Principal component	起始特征值Initial eigenvalues			提取平方和载入Extract the sum of squares and load
主成分 Principal component	特征值 Eigenvalue	贡献率 Contribution rate/%	累积贡献率 Cumulative contribution rate/%	特征值 Eigenvalue	贡献率 Contribution rate/%	累积贡献率 Cumulative contribution rate/%
1	2.217 1.056	55.419	55.419	2.217	55.419	55.419
2	2.217 1.056	26.389	81.809	1.056	26.389	81.809
3	0.471	11.773	93.582
4	0.257	6.418	100.00

主成分 Principal component	起始特征值Initial eigenvalues			提取平方和载入Extract the sum of squares and load
主成分 Principal component	特征值 Eigenvalue	贡献率 Contribution rate/%	累积贡献率 Cumulative contribution rate/%	特征值 Eigenvalue	贡献率 Contribution rate/%	累积贡献率 Cumulative contribution rate/%
1	2.217 1.056	55.419	55.419	2.217	55.419	55.419
2	2.217 1.056	26.389	81.809	1.056	26.389	81.809
3	0.471	11.773	93.582
4	0.257	6.418	100.00

抗寒性指标 Cold resistance index	负荷量Load capacity		权重 Weight/W
抗寒性指标 Cold resistance index	1	2	权重 Weight/W
Fv/Fm	0.914	-0.058	0.269541
REC	0.824	0.168	0.289948
SOD	0.807	-0.372	0.169910
MDA	0.228	0.941	0.270601