香蕉水通道蛋白SIP2-1基因的克隆和表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.012

生物技术通报 ›› 2016, Vol. 32 ›› Issue (7): 81-86.doi: 10.13560/j.cnki.biotech.bull.1985.2016.07.012

香蕉水通道蛋白SIP2-1基因的克隆和表达分析

许奕^{1, 2}, 侯晓婉³, 徐碧玉², 金志强^{1, 2}, 胡伟², 宋顺¹

1. 中国热带农业科学院海口实验站海南省香蕉遗传改良重点实验室,海口 570102；
2. 中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海口 571101；
3. 中国热带农业科学院南亚热带作物研究所,湛江 524091

收稿日期:2015-10-22 出版日期:2016-07-25 发布日期:2016-07-25
作者简介:许奕,女,硕士,研究方向：作物遗传育种;E-mail：lukydog163@163.com
基金资助:
国家自然科学基金项目（31071788,31501371）,海南省自然科学基金项目（314099）,现代农业产业技术体系建设专项资金项目（CARS-32）,“十二五”农村领域国家科技计划项目（2011AA10020605）

Molecular Cloning and Expression Analysis of Aquaporins SIP2-1 Gene from Banana

XU Yi^{1, 2}, HOU Xiao-wan³, XU Bi-yu², JIN Zhi-qiang^{1, 2}, HU Wei², SONG Shun¹

1. Hainan Key Laboratory of Banana Genetic Improvement,Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou 570102;
2. Key Laboratory of Biology and Genetic Resources of Tropical Crops,Institute of Tropical Bioscience and Biotechnology （Ministry of Agriculture）,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101;
3. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091

Received:2015-10-22 Published:2016-07-25 Online:2016-07-25

摘要/Abstract

摘要： 从香蕉中克隆了一个水通道蛋白（AQP）基因MaSIP2-1。序列分析表明,该基因存在一个完整的开放阅读框（ORF）717 bp,编码 239个氨基酸。多序列比对和进化树分析表明,MaSIP2-1所编码的蛋白与其他植物中AQP编码的蛋白具有较高的一致性。其中与马来西亚野生香蕉、油棕、麻风树、野茶树的AQP编码的氨基酸序列的同源性较高,分别为98%、74%、65%和63%。器官特异性分析表明,MaSIP2-1在香蕉的根、茎、叶片、花和果实中均有所表达,其中在茎中表达量较高。通过对其在干旱、高盐、低温、涝害胁迫下的表达结果分析显示,该基因响应干旱、高盐、涝害3种胁迫。

关键词: 水通道蛋白, SIP, 香蕉, 生物信息学, 胁迫, 荧光定量PCR

Abstract: In the present study,we isolated an aquaporin gene from banana,and designated as MaSIP2-1. MaSIP2-1’s complete ORF was 717 bp,and it encoded 239 amino acids. Alignment of amino acid sequences and phylogenetic analysis indicated that the protein encoded by MaSIP2-1 was in high similarity with AQP-encoding protein in the other known plants;and highly homologous with amino acid sequences in Musa acuminate,Elaeis guineensis,Jatropha curcas,and Camellia sinensis by 98%,74%,65%,and 63%,respectively. Organ-specific analysis showed that MaSIP2-1 constitutively expressed in roots,stems,leaves,flowers and fruits,and the highest in stems. Stress analysis demonstrated that MaSIP2-1 responded to the stress such as the drought,salt and waterlogging.

Key words: aquaporins, SIP, banana, bioinformatics, stress, real-time PCR

许奕, 侯晓婉, 徐碧玉, 金志强, 胡伟, 宋顺. 香蕉水通道蛋白SIP2-1基因的克隆和表达分析[J]. 生物技术通报, 2016, 32(7): 81-86.

XU Yi, HOU Xiao-wan, XU Bi-yu, JIN Zhi-qiang, HU Wei, SONG Shun. Molecular Cloning and Expression Analysis of Aquaporins SIP2-1 Gene from Banana[J]. Biotechnology Bulletin, 2016, 32(7): 81-86.

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香蕉水通道蛋白SIP2-1基因的克隆和表达分析

Molecular Cloning and Expression Analysis of Aquaporins SIP2-1 Gene from Banana

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