生物技术通报 ›› 2021, Vol. 37 ›› Issue (11): 72-80.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0444

• 食用菌生物技术专题(专题主编: 黄晨阳) • 上一篇    下一篇

虫草素抗三阴性乳腺癌的转录组学分析

黄海辰1(), 吴文雅1,2, 戚梦1,2, 薛帆正1, 吴小平1,2, 张君丽3, 傅俊生1,2()   

  1. 1.福建农林大学生命科学学院,福州 350002
    2.福建农林大学菌物研究中心,福州 350002
    3.西藏自治区农牧科学院蔬菜研究所,拉萨 850000
  • 收稿日期:2021-04-07 出版日期:2021-11-26 发布日期:2021-12-03
  • 作者简介:黄海辰,男,研究方向:食用菌活性物质研究;E-mail: 2484333267@qq.com
  • 基金资助:
    国家自然科学基金项目(81503187);特色食用菌种质资源创新研究与菌种繁育关键技术集成示范(XZ202001ZY0041N)

RNA-Seq Analysis of Cordycepin Against Triple-negative Breast Cancer

HUANG Hai-chen1(), WU Wen-ya1,2, QI Meng1,2, XUE Fan-zheng1, WU Xiao-ping1,2, ZHANG Jun-li3, FU Jun-sheng1,2()   

  1. 1. College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002
    2. Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002
    3. Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850000
  • Received:2021-04-07 Published:2021-11-26 Online:2021-12-03

摘要:

前期研究表明,蛹虫草活性成分虫草素能有效抑制乳腺癌移植瘤的生长和转移,但其作用机制未清楚。本研究取三阴性乳腺癌移植瘤样品,通过转录组测序技术分析虫草素抗乳腺癌移植瘤的分子作用机制。结果表明,以P<0.05、log2|FC|>1为标准,筛选获得584个差异表达基因,其中上调基因69个,下调基因515个;KEGG分析表明,虫草素对乳腺癌移植瘤的分子调控涉及Hippo signaling pathway、Hedgehog signaling pathway、ECM-receptor interction、TGF-beta signaling pathway等多条癌症相关信号通路;GO分析表明,这些差异基因富集在biological adhesion、growth、metabolic process、locomotion和biological process等16个生物学过程中,提示虫草素对乳腺癌移植瘤生长与转移有极显著的调节作用;进一步对生长和转移过程的基因进行PPI互作分析,提示Hedgehog signaling pathway、Hippo signaling pathway是虫草素抗三阴性乳腺癌的重要潜在作用通路。研究结果提示Hippo-Yap/TAZ信号通路和Hedgehog-Gli信号通路为潜在作用通路,为进一步理解虫草素治疗三阴性乳腺癌的分子机制奠定了基础。

关键词: 虫草素, 转录组, 三阴性乳腺癌, Hedgehog, Hippo

Abstract:

Previous studies have shown that cordycepin,the active ingredient of Cordyceps militaris,can effectively inhibit the growth and metastasis of triple-negative breast cancer xenografts tumor,however its mechanism is not clear yet,thus the purpose of this paper is to predict its mechanism. Taking samples of triple-negative breast cancer xenografts tumor,the molecular mechanism of cordycepin against breast cancer xenografts tumor was analyzed through transcriptome sequencing technology. The results showed that 584 differentially expressed genes were screened using P<0.05,log2|FC|>1 as the standard,including 69 up-regulated genes and 515 down-regulated genes. KEGG analysis demonstrated that cordycepin had molecular regulation on breast cancer xenografts tumors including Hippo signaling pathway,Hedgehog signaling pathway,ECM-receptor interaction,TGF-beta signaling pathway and other cancer-related signaling pathways. GO analysis revealed that these differential genes were enriched in 16 biological processes,such as biological adhesion,growth,metabolic process,and locomotion,etc. It is suggested that cordycepin had significant regulatory effect on the growth and metastasis of breast cancer xenografts tumors. Further protein-protein interaction analysis of these genes in the growth and metastasis process indicated that Hedgehog signaling pathway and Hippo signaling pathway were important potential ones for cordycepin against triple-negative breast cancer. The research results suggest that the Hippo-Yap/TAZ signaling pathway and the Hedgehog-Gli signaling pathway are potential ones,laying the foundation for exploring the molecular mechanism of cordycepin in the treatment of triple-negative breast cancer.

Key words: cordycepin, RNA-Seq, triple negative breast cancer, Hedgehog, Hippo