二斑叶螨取食抗、感螨木薯品种对茉莉酸信号途径基因表达的影响

doi:10.13560/j.cnki.biotech.bull.1985.2021-0993

生物技术通报 ›› 2022, Vol. 38 ›› Issue (6): 211-220.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0993

二斑叶螨取食抗、感螨木薯品种对茉莉酸信号途径基因表达的影响

韩志玲¹^,²^,³(), 陈青²^,³(), 梁晓²^,³, 伍春玲²^,³, 刘迎²^,³, 伍牧锋²^,³, 徐雪莲²

1.海南大学植物保护学院,海口 570228
2.中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带作物病虫害生物防治工程技术研究中心,海口 571101
3.中国热带农业科学院三亚研究院/海南省南繁生物安全与分子育种重点实验室,三亚 572000

收稿日期:2021-08-06 出版日期:2022-06-26 发布日期:2022-07-11
作者简介:韩志玲,女,硕士,研究方向：抗虫种质资源挖掘与创新利用;E-mail： hb18831321679@126.com
基金资助:
国家重点研发计划专项(2020YFD1000603);国家木薯产业技术体系虫害防控岗位科学家专项(CARS-11-HNCQ);农业农村部农业资源调查与保护利用专项(NFZX-2021);海南省重大科技计划项目(ZDKJ202002)

Influence on Expression of Jasmonic Acid Signaling Pathway Gene in Tetranychus urticae Fed on Mite-resistant and Mite-susceptible Cassava Cultivars

HAN Zhi-ling¹^,²^,³(), CHEN Qing²^,³(), LIANG Xiao²^,³, WU Chun-ling²^,³, LIU Ying²^,³, WU Mu-feng²^,³, XU Xue-lian²

1. College of Plant Protection of Hainan University,Haikou 570228
2. Environment and Plant Protection Institute,China Academy of Tropical Agricultural Sciences/Key Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Agriculture and Rural Affairs/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests,Haikou 571101
3. Sanya Institute,China Academy of Tropical Agricultural Sciences/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions,Sanya 572000

Received:2021-08-06 Published:2022-06-26 Online:2022-07-11

摘要/Abstract

摘要：

明确二斑叶螨取食影响木薯茉莉酸信号途径基因表达的害螨密度和取食时间效应,为阐明茉莉酸信号途径在木薯抗虫性中的重要作用及抗螨种质资源鉴定评价提供理论依据及参考指标。以抗螨木薯品种C1115和感螨木薯品种面包为材料,分析与植物防御相关的茉莉酸号信号途径基因（DAD1、LOX2、OPR3和JAR1）在二斑叶螨以不同虫口密度（15、25、35、45、50和55头螨/叶）取食不同时间（1、2、4和8 d）后的表达量变化情况。结果表明,以45-50头螨/叶的害螨密度分别取食抗、感螨木薯品种2 d后,LOX2、OPR3和JAR1的表达量均较螨害前显著提高,并且上述3个基因在抗螨木薯品种中的表达量显著高于感螨木薯品种,而DAD1的表达量随害螨密度的提高和取食时间的增加波动较大。在较高的害螨密度和较短的为害时间处理时,茉莉酸信号途径基因LOX2、OPR3和JAR1在抗螨木薯品种中的表达量显著高于感螨木薯品种,LOX2、OPR3和JAR1可能与木薯对二斑叶螨的抗性有关。

关键词: 二斑叶螨, 抗、感木薯品种, 害螨密度, 取食时间, 茉莉酸途径, 基因表达

Abstract:

This study aims to clarify how the mite density and feeding time affect gene expressions of jasmonic acid signaling pathway when Tetranychus urticae fed on mite-resistant and mite-susceptible cassava cultivars,which can not only provide a theoretical basis for elucidating the important role of jasmonic acid signal pathway in cassava resistance to insect,but also can offer references for identifying and evaluating mite resistant germplasm resources. Here,mites with different densities（15,25,35,45,50 and 55 mites/leaf）were inoculated on the leaves of mite-resistant cassava cultivar C1115 and mite-susceptible cassava cultivar Mianbao,respectively. And then the expressions of jasmonic acid signaling pathway genes（DAD1,LOX2,OPR3 and JAR1）were analyzed at 1,2,4 and 8 d post-infestation,respectively. The results showed that the expressions of LOX2,OPR3 and JAR1 in 2 d post-infestation either mite-resistant cassava C1115 and mite-susceptible cassava Mianbao significantly increased compared with those before infested,in addition,the expressions of those three genes were significantly higher in C1115 than Mianbao（P<0.05）,while the expressions of DAD1 showed great fluctuation as the mite density and infestation time increased. This study revealed that under the conditions of relative high mite density and short mite infestation period,the expressions of jasmonic acid signaling pathway genes like LOX2,OPR3 and JAR1 were significantly higher in mite-resistant cassava cultivars compared with the susceptible ones. Those genes might probably correlate with cassava resistance to mite,which needs further investigation.

Key words: Tetranychus urticae, mite-resistant and mite-susceptible cassava cultivars, mite density, mite infestation time, jasmonic acid signaling pathway, gene expression

韩志玲, 陈青, 梁晓, 伍春玲, 刘迎, 伍牧锋, 徐雪莲. 二斑叶螨取食抗、感螨木薯品种对茉莉酸信号途径基因表达的影响[J]. 生物技术通报, 2022, 38(6): 211-220.

HAN Zhi-ling, CHEN Qing, LIANG Xiao, WU Chun-ling, LIU Ying, WU Mu-feng, XU Xue-lian. Influence on Expression of Jasmonic Acid Signaling Pathway Gene in Tetranychus urticae Fed on Mite-resistant and Mite-susceptible Cassava Cultivars[J]. Biotechnology Bulletin, 2022, 38(6): 211-220.

图/表 5

表1 木薯JA信号途径基因qPCR分析的引物

Table 1 qPCR primers for the the expression analysis of jasmonic acid（JA）pathway genes in cassava

引物名称 Primer name	基因登录号Gene ID	序列Sequence（5'-3'）	退火温度Annealing temperature Tm/℃	产物大小Product size/bp
OPR3-F	MANES_03G039700	GTTCACACCTCCAAGGCGA	57.2	103
OPR3-R	MANES_03G039700	AACTCCATCAAACCCAGCTT	58.2	103
DAD1-F	MANES_01G274500	AATGGGCCAATGGTGGAGAG	57.5	118
DAD1-R	MANES_01G274500	CACCGTACCTTTCCAGGACC	59.5	118
JAR1-F	MANES_05G025500	TCATCTACTACGCCCAAGTTTAAGA	58.4	118
JAR1-R	MANES_05G025500	CCATCTCAGCGCCCTTTGTA	58.5	118
LOX2-F	MANES_10G148800	GCCATGGAAACACTTCTTGCT	55.9	297
LOX2-R	MANES_10G148800	CTAGCCCCGTTTTGGGATCA	57.6	297
MeTub-F	MANES_18G123607	ATGTTGAGTTCAATATGCAGATCC	58.1	154
MeTub-R	MANES_18G123607	CCCAGAACTTGGCTCCGATT	61.7	154

图1 不同密度二斑叶螨为害不同时间后OPR3的相对表达量 A：C1115;B：面包;C：C1115 vs面包。不同小写字母表示在不同密度二斑叶螨为害相同时间后,木薯OPR3表达量具有显著差异（P<0.05）,不同大写字母表示相同密度二斑叶螨为害不同时间后,OPR3表达量具有显著差异（P<0.05）。下同

Fig. 1 Relative transcriptions of OPR3 gene in mite-resis-tant and mite-susceptible cassava cultivars after infested by mites of different densities at different time A：C1115. B：Mianbao. C：C1115 vs Mianbao. Different lowercase letters indicate that there are significant differences in transcription of OPR3 when cassava was infested by mites of different densities at the same time（P<0.05）,and different capital letters indicate there are significant differences in transcription of OPR3 when cassava were infested by the mites of the same densities at different time（P < 0.05）. The same below

图2 不同密度二斑叶螨为害不同时间后LOX2的相对表达量

Fig. 2 Relative transcriptions of LOX2 gene in mite-resistant and mite-susceptible cassava cultivars after infested by the mites of different densities at different time

图3 不同密度二斑叶螨为害不同时间后DAD1的相对表达量

Fig. 3 Relative transcriptions of DAD1 gene in mite-resistant and mite-susceptible cassava cultivars after infested by the mites of different densities at different time

图4 不同密度二斑叶螨为害不同时间后JAR1的相对表达量

Fig. 4 Relative transcriptions of JAR1 gene in mite-resistant and mite-susceptible cassava cultivars after infested by the mites of different densities at different time

参考文献 47

[1]	朱柏光, 李闯, 张雪娇, 等. 木薯MeSWEET3b的基因克隆及功能分析[J]. 分子植物育种, 2022, 20(3):733-741.
	Zhu BG, Li C, Zhang XJ, et al. Molecular cloning and function analysis of a MeSWEET3b gene from Manihot esculenta[J]. Mol Plant Breed, 2022, 20(3):733-741.
[2]	Hu W, Ji C, Shi H, et al. Allele-defined genome reveals biallelic differentiation during cassava evolution[J]. Mol Plant, 2021, 14(6):851-854. doi: 10.1016/j.molp.2021.04.009 URL
[3]	张哲, 陈青, 梁晓, 等. 朱砂叶螨为害前后抗、感木薯品种叶组织营养物质含量差异分析[J]. 热带作物学报, 2020, 41(9):1865-1869.
	Zhang Z, Chen Q, Liang X, et al. Analysis on difference of nutrient content in leaf tissue of resistant and sensitive cassava varieties before and after damage to Tetranychus cinnabarinus[J]. Chin J Trop Crops, 2020, 41(9):1865-1869.
[4]	肖鑫辉, 叶剑秋, 王明, 等. 木薯种质资源淀粉特性分析与评价[J]. 热带作物学报, 2021, 42(2):339-348.
	Xiao XH, Ye JQ, Wang M, et al. Analysis on starch properties in cassava germplasm resources[J]. Chin J Trop Crops, 2021, 42(2):339-348.
[5]	杨丹. 海南木薯产业发展与影响因素实证分析[D]. 海口: 海南大学, 2017.
	Yang D. An empirical study on the development and effect factors of Hainan cassava industry[D]. Haikou: Hainan University, 2017.
[6]	王国芬, 李超萍, 时涛, 等. 我国木薯花叶病毒病的发生危害及其病原鉴定[J]. 热带作物学报, 2021, 42(6):1668-1677.
	Wang GF, Li CP, Shi T, et al. Distribution and pathogen detection of cassava mosaic virus disease in China[J]. Chin J Trop Crops, 2021, 42(6):1668-1677.
[7]	金晶, 杨顺义, 尚素琴, 等. 苦参碱对二斑叶螨解毒酶活力的影响[J]. 草原与草坪, 2020, 40(6):89-94.
	Jin J, Yang SY, Shang SQ, et al. The effect of matrine on detoxification enzymes in Tetranychus urticae[J]. Grassland Turf, 2020, 40(6):89-94.
[8]	胡展育, 郅军锐. 二斑叶螨的研究进展[J]. 山地农业生物学报, 2004, 23(5):442-447.
	Hu ZY, Zhi JR. Review of the researches on Tetranychus urticae Koch[J]. J Mt Agric Biol, 2004, 23(5):442-447.
[9]	Chen Q, Liang X, Wu C, et al. Density threshold-based acaricide application for the two-spotted spider mite Tetranychus urticae on cassava:from laboratory to the field[J]. Pest Manag Sci, 2019, 75(10):2634-2641. doi: 10.1002/ps.5366 URL
[10]	王莹, 杨开选, 周爱英. 大棚冬枣二斑叶螨的发生与防治[J]. 陕西林业科技, 2019, 47(4):71-72, 75.
	Wang Y, Yang KX, Zhou AY. Control of Tetranychus urticae infecting Zizyphus jujube cv. Dongzao in greenhouse[J]. Shaanxi For Sci Technol, 2019, 47(4):71-72, 75.
[11]	蒋立奔, 曹荣祥, 童晓利, 等. 不同杀螨剂对二斑叶螨的室内毒力及田间防效[J]. 江苏农业科学, 2019, 47(23):116-118.
	Jiang LB, Cao RX, Tong XL, et al. Indoor toxicity test and field efficacy of different acaricides against Tetranychus urticae Koch[J]. Jiangsu Agric Sci, 2019, 47(23):116-118.
[12]	刘艳潇, 祝一鸣, 周而勋. 植物免疫诱抗剂的作用机理和应用研究进展[J]. 分子植物育种, 2020, 18(3):1020-1026.
	Liu YX, Zhu YM, Zhou EX. Research progress on the action mechanism and application of plant immune inducers[J]. Mol Plant Breed, 2020, 18(3):1020-1026.
[13]	张杰, 董莎萌, 王伟, 等. 植物免疫研究与抗病虫绿色防控:进展、机遇与挑战[J]. 中国科学:生命科学, 2019, 49(11):1479-1507.
	Zhang j, Dong SM, Wang W, et al. Plant immunity and sustainable control of pests in China:Advances, opportunities and challenges[J]. Sci Sin:Vitae, 2019, 49(11):1479-1507.
[14]	唐威华, 冷冰, 何祖华. 植物抗病虫与抗逆[J]. 植物生理学报, 2017, 53(8):1333-1336.
	Tang WH, Leng B, He ZH. Biotic and abiotic stress resistance in plants[J]. Plant Physiol J, 2017, 53(8):1333-1336.
[15]	黄俊, 王帅, 张云川, 等. 植物激素茉莉酸调控天然橡胶合成的研究进展[J]. 植物生理学报, 2020, 56(4):654-660.
	Huang J, Wang S, Zhang YC, et al. Advances in the regulation of natural rubber synthesis by the phytohormone jasmonic acid[J]. Plant Physiol J, 2020, 56(4):654-660.
[16]	焦龙, 蔡晓明, 边磊, 等. 茉莉酸类化合物:从植物的诱导抗虫防御反应到生长-防御权衡[J]. 应用生态学报, 2018, 29(11):3876-3890.
	Jiao L, Cai XM, Bian L, et al. Jasmonates:From induced plant anti-herbivore defensive reaction to growth-defense tradeoffs[J]. Chin J Appl Ecol, 2018, 29(11):3876-3890.
[17]	黎家, 李传友. 新中国成立70年来植物激素研究进展[J]. 中国科学:生命科学, 2019, 49(10):1227-1281.
	Li J, Li CY. Seventy-year major research progress in plant hormones by Chinese scholars[J]. Sci Sin:Vitae, 2019, 49(10):1227-1281.
[18]	段龙飞. 茉莉酸信号途径上关键基因家族COI/JAZ/MYC分子进化分析[D]. 杨凌: 西北农林科技大学, 2013.
	Duan LF. Molecular evolutionary analysis of the key gene families COI/JAZ/MYC in jasmonic acid signaling pathway[D]. Yangling: Northwest A & F University, 2013.
[19]	范东哲, 陈青, 梁晓, 等. 桃蚜取食对抗、感蚜辣椒品种水杨酸、茉莉酸信号途径的影响[J]. 热带作物学报, 2021, 42(10):2972-2978.
	Fan DZ, Chen Q, Liang X, et al. Myzus persicae feeding affects salicylic acid and jasmonic acid signaling pathways in aphid-resistant and aphid-susceptible pepper cultivars[J]. Chin J Trop Crops, 2021, 42(10):2972-2978.
[20]	崔伟康, 杨鹏, 廉法卓, 等. 茉莉酸信号转导途径介导番茄对棉铃虫的抗性[J]. 福建农林大学学报:自然科学版, 2016, 45(4):371-375.
	Cui WK, Yang P, Lian FZ, et al. Jasmonate signaling pathway mediates tomato resistance against Helicoverpa armigera[J]. J Fujian Agric For Univ:Nat Sci Ed, 2016, 45(4):371-375.
[21]	冯建雄, 常静, 李海平, 等. 外源茉莉酸诱导对油菜防御酶活性和4种物质含量及植株生长的影响[J]. 植物保护, 2021, 47(1):128-134, 187.
	Feng JX, Chang J, Li HP, et al. Effects of exogenous jasmonic acid on the activities of defense enzymes, the contents of four substances and the growth of rape[J]. Plant Prot, 2021, 47(1):128-134, 187.
[22]	冯建雄. 外源茉莉酸对3种油菜幼苗的诱导防御反应[D]. 呼和浩特: 内蒙古农业大学, 2019.
	Feng JX. Defense responses of 3 rape seedling leaves induced by exogenous jasmonic acid[D]. Hohhot: Inner Mongolia Agricultural University, 2019.
[23]	张晓霞, 洪波, 景亮亮, 等. 外源茉莉酸甲酯诱导番茄对烟粉虱的抗虫性[J]. 生态学杂志, 2020, 39(11):3651-3657.
	Zhang XX, Hong B, Jing LL, et al. Insect resistance of tomato induced by exogenous methyl jasmonate to Bemisia tabaci[J]. Chin J Ecol, 2020, 39(11):3651-3657.
[24]	张蕊蕊. 媒介虫口密度、植株苗龄及温度对烟粉虱传播台湾番茄曲叶病毒的影响[D]. 杭州: 浙江大学, 2011.
	Zhang RR. Effect of vector density, plant age and temperature on the transmission of tomato leaf curl Taiwan virus by the whitefly Bemisia tabaci[D]. Hangzhou: Zhejiang University, 2011.
[25]	马雯, 何云川, 王新谱. 不同虫口密度Q型烟粉虱成虫对番茄幼苗生理指标的影响[J]. 农业科学研究, 2020, 41(3):7-11.
	Ma W, He YC, Wang XP. Effects of Bemisia tabaci Q-type feeding on physiological index of Solanum lycopersicum under different population densities[J]. J Agric Sci, 2020, 41(3):7-11.
[26]	任倩倩. 抗、感茶树品种对茶小绿叶蝉取食诱导的防御反应[D]. 福州: 福建农林大学, 2020.
	Ren QQ. The defense strategies of resistant and susceptible tea cultivars in response to Empoasca onukii feeding[D]. Fuzhou: Fujian Agriculture and Forestry University, 2020.
[27]	赵文娟, 夏爽, 郑薇薇, 等. 益害比及花粉存在对江原钝绥螨和柑橘全爪螨种群动态的影响[J]. 植物保护, 2021, 47(1):148-152.
	Zhao WJ, Xia S, Zheng WW, et al. Effects of predatory mite-pest mite ratio and pollen presence on population dynamics of Amblyseius eharai and Panonychus citri[J]. Plant Prot, 2021, 47(1):148-152.
[28]	李红, 陈鑫, 许艺, 等. 益生菌EG-2#不同浓度处理对辣椒苗期生长发育的影响研究[J]. 四川农业科技, 2020(8):14-15.
	Li H, Chen X, Xu Y, et al. Effects of probiotic eg-2# different concentrations on the growth and development of pepper seedlings[J]. Sichuan Agric Sci Technol, 2020(8):14-15.
[29]	李迁, 卢芙萍, 陈青, 等. 木薯种质对朱砂叶螨的抗性评价[J]. 热带作物学报, 2015, 36(1):143-151.
	Li Q, Lu FP, Chen Q, et al. Evaluation of cassava germplasms for resistance to spider mite Tetranychus cinnabarinus(Acari:Tetranychidae)[J]. Chin J Trop Crops, 2015, 36(1):143-151.
[30]	周兴隆, 杨顺义, 郝雨, 等. 二斑叶螨多重抗性品系最优内参基因的筛选及CYP392A亚家族基因的表达分析[J]. 昆虫学报, 2015, 58(11):1229-1236.
	Zhou XL, Yang SY, Hao Y, et al. Selection of the most suitable reference genes and expression profiling of CYP392A subfamily genes in the multi-pesticide resistant strain of Tetranychus urticae(Acari:Tetranychidae)[J]. Acta Entomol Sin, 2015, 58(11):1229-1236.
[31]	Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-^ΔΔCT method[J]. Methods, 2001, 25(4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[32]	宋云, 李林宣, 卓凤萍, 等. 茉莉酸信号传导在植物抗逆性方面研究进展[J]. 中国农业科技导报, 2015, 17(2):17-24.
	Song Y, Li LX, Zhuo FP, et al. Progress on jasmonic acid signaling in plant stress resistant[J]. J Agric Sci Technol, 2015, 17(2):17-24.
[33]	Yates-Stewart AD, Pekarcik A, Michel A, et al. Jasmonic acid-isoleucine(ja-ile)is involved in the host-plant resistance mechanism against the soybean aphid(hemiptera:Aphididae)[J]. J Econ Entomol, 2020, 113(6):2972-2978. doi: 10.1093/jee/toaa221 pmid: 33033836
[34]	刘庆霞, 李梦莎, 国静. 茉莉酸生物合成的调控及其信号通路[J]. 植物生理学报, 2012, 48(9):837-844.
	Liu QX, Li MS, Guo J. Regulation of jasmonic acid biosynthesis and jasmonic acid signaling pathway[J]. Plant Physiol J, 2012, 48(9):837-844.
[35]	王文艳. 葡萄水杨酸和茉莉酸信号转导途径中4个重要基因的克隆及表达分析[D]. 南京: 南京农业大学, 2011.
	Wang WY. Cloning and expression analysis of four important grapevine genes involved in sa and ja signalling pathways[D]. Nanjing: Nanjing Agricultural University, 2011.
[36]	张镇川, 何冰, 李甜, 等. 冠菌素对番茄防御基因表达、胼胝质沉积及细菌生长的影响[J]. 四川农业大学学报, 2021, 39(1):27-34.
	Zhang ZC, He B, Li T, et al. The effect of coronatine on tomato defense gene expression, callose deposition and bacterial growth[J]. J Sichuan Agric Univ, 2021, 39(1):27-34.
[37]	韦晓霞, 王小安, 陈瑾, 等. 百香果低温胁迫转录组及茉莉酸代谢基因分析[J]. 核农学报, 2021, 35(4):815-825. doi: 10.11869/j.issn.100-8551.2021.04.0815
	Wei XX, Wang XA, Chen J, et al. Transcriptome and jasmin metabolism gene analysis of Passiflora edulia Sims under low temperature stress[J]. J Nucl Agric Sci, 2021, 35(4):815-825.
[38]	彭淑萍, 董诚明, 朱畇昊. 响应内生菌侵染的两个地黄茉莉酸合成关键基因的克隆与表达分析[J]. 植物研究, 2021, 41(2):294-301.
	Peng SP, Dong CM, Zhu YH. Cloning and expression analysis of two key genes of jasmonic acid synthesis in response to endophytic infection from Rehmannia glutinosa[J]. Bull Bot Res, 2021, 41(2):294-301.
[39]	贾庆利. 辣椒与黄瓜抗疫病相关基因克隆及分析[D]. 杨凌: 西北农林科技大学, 2012.
	Jia QL. Cloning and analysis of Phytophthora blight resistance-rjelated genes in pepper and cucumber[D]. Yangling: Northwest A & F University, 2012.
[40]	郭航. 茄子茉莉酸生物合成途径相关基因的克隆与表达分析[D]. 重庆: 西南大学, 2015.
	Guo H. Cloning and expression analysisi of jasmonic acid biosynthesis pathway genes in Solanum melongena L[D]. Chongqing: Southwest University, 2015.
[41]	牟艺菲. 小麦OPR和LOX基因家族的鉴定及其抗逆功能分析[D]. 杨凌: 西北农林科技大学, 2019.
	Mou YF. Identification and functional analysis of wheat OPR and LOX gene families in response to stresses[D]. Yangling: Northwest A & F University, 2019.
[42]	陈慧. 茶树被茶尺蠖取食诱导的一个13-脂氧合酶基因的分离、功能鉴定与表达分析[D]. 合肥: 安徽农业大学, 2011.
	Chen H. Identification and characterization of a herbivore-induced lipoxygenase gene in the leaves of Camellia sinensis fed by Ectropis obliqua[D]. Hefei: Anhui Agricultural University, 2011.
[43]	乔菊香, 吴嘉, 张国斌, 等. 拟南芥茉莉酸信号途径突变体jar1的灰葡萄孢菌抗性分析[J]. 分子植物育种, 2020, 18(12):4009-4013.
	Qiao JX, Wu J, Zhang GB, et al. Resistant analysis of jasmonic acid signaling mutant jar1 to Botrytis cinerea in Arabidopsis[J]. Mol Plant Breed, 2020, 18(12):4009-4013.
[44]	Guo HM, Li HC, Zhou SR, et al. Cis-12-oxo-phytodienoic acid stimulates rice defense response to a piercing-sucking insect[J]. Mol Plant, 2014, 7(11):1683-1692. doi: 10.1093/mp/ssu098 URL
[45]	张海波. 烟粉虱Bemisia tabaci(Gennadius)取食及外源JA处理对辣椒(Capisicum annuum L.)内源JA、SA的影响[D]. 扬州: 扬州大学, 2018.
	Zhang HB. Effects of Bemisia tabaci(Gennadius)feeding and exogenous JA treatment on endogenous JA and SA of Capsicum annuum L.[D]. Yangzhou: Yangzhou University, 2018.
[46]	张羽宇. 西花蓟马取食对菜豆植株系统抗性的诱导[D]. 贵阳: 贵州大学, 2017.
	Zhang YY. The systemic resistance of the kidney bean induced by Frankliniella occidentalis(Pergande)(Thysanoptera:Thripidae)[D]. Guiyang: Guizhou University, 2017.
[47]	刘勇. 钙素在西花蓟马取食诱导菜豆防御反应中的作用[D]. 贵阳: 贵州大学, 2016.
	Liu Y. The role of calcium in kidney bean defensive responses induced by western flower thrip feeding[D]. Guiyang: Guizhou University, 2016.

二斑叶螨取食抗、感螨木薯品种对茉莉酸信号途径基因表达的影响

Influence on Expression of Jasmonic Acid Signaling Pathway Gene in Tetranychus urticae Fed on Mite-resistant and Mite-susceptible Cassava Cultivars

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 47

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杨志晓, 侯骞, 刘国权, 卢志刚, 曹毅, 芶剑渝, 王轶, 林英超. 不同抗性烟草品系Rubisco及其活化酶对赤星病胁迫的响应[J]. 生物技术通报, 2023, 39(9): 202-212.
[2]	李帜奇, 袁月, 苗荣庆, 庞秋颖, 张爱琴. 盐胁迫盐芥和拟南芥褪黑素含量及合成相关基因表达模式分析[J]. 生物技术通报, 2023, 39(5): 142-151.
[3]	刘奎, 李兴芬, 杨沛欣, 仲昭晨, 曹一博, 张凌云. 青杄转录共激活因子PwMBF1c的功能研究与验证[J]. 生物技术通报, 2023, 39(5): 205-216.
[4]	赖瑞联, 冯新, 高敏霞, 路喻丹, 刘晓驰, 吴如健, 陈义挺. 猕猴桃过氧化氢酶基因家族全基因组鉴定与表达分析[J]. 生物技术通报, 2023, 39(4): 136-147.
[5]	郭三保, 宋美玲, 李灵心, 尧子钊, 桂明明, 黄胜和. 斑地锦查尔酮合酶基因及启动子的克隆与分析[J]. 生物技术通报, 2023, 39(4): 148-156.
[6]	陈强, 邹明康, 宋家敏, 张冲, 吴隆坤. 甜瓜LBD基因家族的鉴定和果实发育进程中的表达分析[J]. 生物技术通报, 2023, 39(3): 176-183.
[7]	姚晓文, 梁晓, 陈青, 伍春玲, 刘迎, 刘小强, 税军, 乔阳, 毛奕茗, 陈银华, 张银东. 二斑叶螨抗性木薯木质素合成途径基因表达特性研究[J]. 生物技术通报, 2023, 39(2): 161-171.
[8]	李彦霞, 王晋鹏, 冯芬, 包斌武, 董益闻, 王兴平, 罗仍卓么. 大肠杆菌型奶牛乳房炎对产奶性状相关基因表达的影响[J]. 生物技术通报, 2023, 39(2): 274-282.
[9]	冯策婷, 江律, 刘鑫颖, 罗乐, 潘会堂, 张启翔, 于超. 单叶蔷薇NAC基因家族鉴定及干旱胁迫响应分析[J]. 生物技术通报, 2023, 39(11): 283-296.
[10]	吴柏增, 何琪, 姚方杰, 赵梦然. 糙皮侧耳乳酸脱氢酶鉴定及其菌丝高温胁迫下表达特征分析[J]. 生物技术通报, 2023, 39(11): 350-359.
[11]	姜南, 石杨, 赵志慧, 李斌, 赵熠辉, 杨俊彪, 闫家铭, 靳雨璠, 陈稷, 黄进. 镉胁迫下水稻OsPT1的表达及功能分析[J]. 生物技术通报, 2023, 39(1): 166-174.
[12]	段敏杰, 李怡斐, 杨小苗, 王春萍, 黄启中, 黄任中, 张世才. 辣椒锌指蛋白DnaJ-Like基因家族鉴定及对高温胁迫的表达响应[J]. 生物技术通报, 2023, 39(1): 187-198.
[13]	袁星, 郭彩华, 刘金明, 亢超, 全绍文, 牛建新. 核桃CONSTANS-Like基因家族全基因组鉴定及表达分析[J]. 生物技术通报, 2022, 38(9): 167-179.
[14]	郭宾会, 宋丽. 大豆孢囊线虫侵染对乙烯合成及信号传导基因表达调控的研究[J]. 生物技术通报, 2022, 38(8): 150-158.
[15]	张淼, 杨露露, 贾岩龙, 王天云. DNA甲基化和组蛋白甲基化修饰的表观遗传调控作用研究进展[J]. 生物技术通报, 2022, 38(7): 23-30.