核桃CONSTANS-Like基因家族全基因组鉴定及表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2021-1459

摘要/Abstract

摘要：

基于核桃基因组鉴定核桃COL（CONSTANS-Like）家族成员,并对其进行分析,以揭示核桃COL家族的分子进化和潜在功能。以‘新新2号'（Juglans regia cv. Xinxin No. 2）为材料,对COL基因家族全基因组进行鉴定及生物信息分析,并分析其在不同组织及不同时间雌花芽的表达。结果表明,核桃基因组中共存在55个COL基因,不均匀地分布在16个染色体和1个Scaffold上,依次命名为JrCOL1-JrCOL55,氨基酸为117-789 aa,分子量为12.99-86.08 kD,等电点为4.00-8.94,进化树分析将其分为3个亚族,且同一分支基因的保守基序和基因结构位置相似。启动子区的顺式作用元件分析发现JrCOL基因含有大量与光信号、植物激素、胁迫等相关的顺式作用元件。‘新新2号'中JrCOL家族基因具有组织特异性表达,其中JrCO1、JrCO7a-c、JrCO8、JrCOL23、JrCO31a-c、JrCO33、JrCOL35和JrCOL50a-f在叶片中表达量最高。对‘新新2号'雌花芽不同时间JrCOL基因的RT-qPCR分析表明,JrCOL1、JrCOL35、JrCOL50a-f和JrCOL52在‘新新2号'雌花芽中,具有一致的表达趋势,其中JrCOL50a-f在‘新新2号'雌花芽分化过程中起到重要作用。在核桃基因组中鉴定出55个COL家族基因,分为3个亚家族,不均匀地分布在16个染色体和1个Scaffold上,结构进化保守,组织表达模式多样。推测JrCOL家族基因在核桃叶片发育过程中发挥重要功能。

关键词: ‘新新2号', CONSTANS-Like, 核桃基因家族, 全基因组鉴定, 基因表达

Abstract:

The genome-wide analysis of the COL family in walnut（Juglans regia）was used to identify the COL（CONSTANS-Like）family members in walnut,and then to reveal molecular evolution and function of the COL family in walnut. Having ‘Xinxin 2'（Juglans regia cv. Xinxin No. 2）as research materials,the genome-wide identification and comprehensive analysis of the COL gene family in walnut were conducted,and their expressions in the female flower bud in different tissues and stages were analyzed. Totally,55 members of COL genes were identified from the whole genome of walnut,and they were unevenly distributed over 16 chromosomes and 1 Scaffold,designated as JrCOL1-JrCOL55,amino acids were 117-789,the molecular weight was between 12.99 to 86.08 kD,and the theoretical isoelectric point ranged from 4.00 to 8.94. The JrCOL proteins were classified into 3 subgroups by evolutionary tree analysis,and the conserved motifs and gene structure of each subgroup were similar. Cis-acting element analysis in the promoter region showed that JrCOLs contained a large number of functional domains related to light signal,plant hormone and stress. Spatial expression pattern analysis showed that JrCOL gene expression was tissue specific and the expression of JrCO1,JrCO7a-c,JrCO8,JrCOL23,JrCO31a-c,JrCO33,JrCOL35 and JrCOL50a-f was the highest in the leaves. And the RT-PCR analysis revealed that JrCOL1,JrCOL35,JrCOL50a-f and JrCOL52 showed consistent expression trend in female flower buds of ‘Xinxin 2',and JrCOL50a-f played an important role in the differential process of female flower buds of ‘Xinxin 2'. And 55 JrCOL members in 3 subgroups were identified in the walnut,and were unevenly distributed in 16 chromosomes and 1 Scaffold,with conserved motifs distributions and diverse expression patterns. It is suggested that JrCOL family gene plays key role in the development of walnut leaves.

Key words: Juglans regia cv. Xinxin No. 2, CONSTANS-Like, gene family in walnut, genome-wide identification, gene expression

袁星, 郭彩华, 刘金明, 亢超, 全绍文, 牛建新. 核桃CONSTANS-Like基因家族全基因组鉴定及表达分析[J]. 生物技术通报, 2022, 38(9): 167-179.

YUAN Xing, GUO Cai-hua, LIU Jin-ming, KANG Chao, QUAN Shao-wen, NIU Jian-xin. Genome-wide Identification of CONSTANS-Like Family Genes and Expression Analysis in Wanlut[J]. Biotechnology Bulletin, 2022, 38(9): 167-179.

图/表 10

参考文献 41

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基因 Gene	上游引物 Forward primer（5'-3'）	下游引物 Reverse primer（5'-3'）
JrCOL1	TTCGAGAAGACGATCCGATACG	CGATCAACTTCCGATCCCATCT
JrCOL7	ATTCCCCTTCTGTGGGTGAATC	TGGCTCATGTTGTTTTCTGCAG
JrCOL8	GTTATGATGCCATCGACAGCAC	TGCCATTCCATTAACGTGAGGA
JrCOL23	CGTGACGGATGTATCGGATTCA	TTTTCTCTGTACCTCAGCACCC
JrCOL31	CTGCAGAAAACAACATGAGCCA	AATGTGAGGCCCAGAATGAACT
JrCOL33	GGTTCACTGGATGTCCTTCTGT	TGAGAAGCATCATCCCAAACCA
JrCOL35	ACCCCAATTTCAGTTCCAAGGT	TATCCGTTCCCGCACTGTAAAA
JrCOL42	GTGGCTCTAAAGATGTCCCACA	TATTCTGTGCCAGTTGCTCCAT
JrCOL50	CTCGTTGCGATCATCTTGTGTC	GATGACACTGAAGAGCCTCCAA
JrCOL52	AGAATTGGGAAATGGCAGGGAT	CCATCGTAAGCAAAGCCGTAAG
18S	GGTCAATCTTCTCGTTCCCTT	TCGCATTTCGCTACGTTCTT

基因 Gene	上游引物 Forward primer（5'-3'）	下游引物 Reverse primer（5'-3'）
JrCOL1	TTCGAGAAGACGATCCGATACG	CGATCAACTTCCGATCCCATCT
JrCOL7	ATTCCCCTTCTGTGGGTGAATC	TGGCTCATGTTGTTTTCTGCAG
JrCOL8	GTTATGATGCCATCGACAGCAC	TGCCATTCCATTAACGTGAGGA
JrCOL23	CGTGACGGATGTATCGGATTCA	TTTTCTCTGTACCTCAGCACCC
JrCOL31	CTGCAGAAAACAACATGAGCCA	AATGTGAGGCCCAGAATGAACT
JrCOL33	GGTTCACTGGATGTCCTTCTGT	TGAGAAGCATCATCCCAAACCA
JrCOL35	ACCCCAATTTCAGTTCCAAGGT	TATCCGTTCCCGCACTGTAAAA
JrCOL42	GTGGCTCTAAAGATGTCCCACA	TATTCTGTGCCAGTTGCTCCAT
JrCOL50	CTCGTTGCGATCATCTTGTGTC	GATGACACTGAAGAGCCTCCAA
JrCOL52	AGAATTGGGAAATGGCAGGGAT	CCATCGTAAGCAAAGCCGTAAG
18S	GGTCAATCTTCTCGTTCCCTT	TCGCATTTCGCTACGTTCTT

基因名称 Gene name	基因ID Gene ID	染色体位置 Chromosome position	相关蛋白 Related protein	蛋白重命名 Protein rename	氨基酸长度 aa length	蛋白分子量 Molecular weight/kD	理论等电点pI	亚细胞定位Subc- ellular localization
JrCOL1	LOC109001350	Chr.1：1256032..1258201	XP_018834143.1	JrCOL1	373	41.31	5.17	叶绿体
JrCOL2	LOC109001381	Chr.1：1488506..1490928	XP_035544637.1	JrCOL2	211	23.40	5.73	细胞质
JrCOL3	LOC109009665	Chr.1：3978995..3983057	XP_018845790.2	JrCOL3	350	39.64	8.72	细胞核
JrCOL4a-d	LOC109010156	Chr.1：18366251..18370500	XP_035543378.1	JrCOL4a	428	47.24	5.27	细胞核
			XP_018846422.1	JrCOL4b	428	47.24	5.27	细胞核
			XP_018846423.1	JrCOL4c	428	47.24	5.27	细胞核
			XP_018846424.1	JrCOL4d	428	47.24	5.27	细胞核
JrCOL5	LOC109015440	Chr.1：18372462..18372860	XP_018853453.2	JrCOL5	132	14.50	6.85	细胞核
JrCOL6a-b	LOC108991987	Chr.1：19317524..19318826	XP_018821963.2	JrCOL6a	237	25.91	4.42	细胞核
			XP_018821964.2	JrCOL6b	236	25.78	4.42	细胞核
JrCOL7a-c	LOC109000761	Chr.1：44957519..44963229	XP_018833298.1	JrCOL7a	416	45.11	5.19	细胞核
			XP_018833299.1	JrCOL7b	416	45.11	5.19	细胞核
			XP_018833303.1	JrCOL7c	416	45.11	5.19	细胞核
JrCOL8	LOC109013302	Chr.2：7141021..7147381	XP_018850883.1	JrCOL8	539	60.48	6.22	细胞核
JrCOL9	LOC108984132	Chr.2：11707792..11709460	XP_018811512.1	JrCOL9	277	31.23	4.94	细胞核
JrCO10	LOC109014521	Chr.2：30680768..30682741	XP_018852564.1	JrCOL10	333	37.49	4.97	细胞核
JrCOL11a-b	LOC109014085	Chr.2：35047335..35048788	XP_018851954.1	JrCOL11a	258	27.76	4.28	细胞核
			XP_018851962.1	JrCOL11b	255	27.37	4.25	细胞核
JrCOL12a-b	LOC108999038	Chr.3：18899526..18901860	XP_018831379.1	JrCOL12a	150	16.21	4.00	细胞核,叶绿体
			XP_035544064.1	JrCOL12b	150	16.21	4.00	细胞核,叶绿体
JrCOL13	LOC109017893	Chr.4：14653613..14655876	XP_035544914.1	JrCOL13	170	18.24	4.11	细胞核
JrCOL14	LOC108989855	Chr.5：6297905..6299253	XP_018819161.1	JrCOL14	220	26.37	4.94	细胞核
JrCOL15a-b	LOC109022307	Chr.5：9188186..9197431	XP_018860727.1	JrCOL15a	789	86.08	6.30	叶绿体
			XP_018860748.1	JrCOL15b	778	84.89	6.35	叶绿体
JrCOL16	LOC108994549	Chr.6：15543376..15545802	XP_018825341.2	JrCOL16	444	48.81	5.88	细胞核
JrCOL17	LOC108998057	Chr.6：29988891..29991120	XP_018830038.2	JrCOL17	407	46.32	5.46	细胞核
JrCOL18	LOC108992642	Chr.7：12544651..12554366	XP_018822785.2	JrCOL18	685	75.72	6.71	细胞核
JrCOL19	LOC108983659	Chr.7：14608063..14609540	XP_018810913.1	JrCOL19	266	28.49	8.73	叶绿体
JrCOL20	LOC109005469	Chr.7：32506953..32517320	XP_018839979.2	JrCOL20	238	26.36	5.08	细胞核
JrCOL21	LOC109019524	Chr.7：38617705..38622031	XP_018857358.1	JrCOL21	432	46.81	4.35	叶绿体
JrCOL22a-b	LOC109014549	Chr.7：49644025..49658252	XP_035548321.1	JrCOL22a	780	85.75	6.42	叶绿体
			XP_035548322.1	JrCOL22b	778	85.50	6.42	叶绿体
JrCOL23	LOC109000996	Chr.8：600112..601757	XP_018833641.1	JrCOL23	345	37.98	6.16	细胞质
JrCOL24	LOC108984911	Chr.8：6608517..6610109	XP_018812557.1	JrCOL24	301	33.04	6.25	细胞核
JrCOL25a-b	LOC108980667	Chr.8：24595513..24597399	XP_035549568.1	JrCOL25a	158	17.12	5.62	细胞质
			XP_035549569.1	JrCOL25b	117	12.99	6.49	细胞质
JrCOL26	LOC108997344	Chr.9：19840907..19842615	XP_018829103.1	JrCOL26	331	37.53	5.83	细胞核
JrCOL27	LOC108985349	Chr.9：21400481..21403221	XP_018813169.1	JrCOL27	295	31.92	5.55	叶绿体
JrCOL28	LOC108986213	Chr.10：2554388..2557653	XP_018814300.1	JrCOL28	299	34.08	6.43	细胞核
JrCOL29a-c	LOC108984383	Chr.10：13263191..13268808	XP_018811858.2	JrCOL29a	436	47.97	6.55	细胞质
			XP_035550266.1	JrCOL29b	436	47.97	6.55	细胞质
			XP_035550267.1	JrCOL29c	408	44.52	5.70	细胞核
JrCOL30a-b	LOC109017472	Chr.10：13911798..13913088	XP_018855279.1	JrCOL30a	260	28.60	4.37	叶绿体
			XP_018855280.1	JrCOL30b	259	28.48	4.37	叶绿体
JrCOL31a-c	LOC109000741	Chr.10：37518173..37524266	XP_018833263.1	JrCOL31a	409	44.45	5.96	细胞核
			XP_018833265.1	JrCOL31b	409	44.45	5.96	细胞核
			XP_018833266.1	JrCOL31c	409	44.45	5.96	细胞核
JrCOL32a-d	LOC108992026	Chr.11：8894066..8897365	XP_018822007.1	JrCOL32a	194	21.42	6.43	叶绿体
			XP_018822008.1	JrCOL32b	192	21.17	6.23	叶绿体
			XP_018822009.1	JrCOL32c	186	20.48	6.43	叶绿体
			XP_018822010.1	JrCOL32d	184	20.22	6.23	叶绿体
JrCOL33	LOC108992144	Chr.11：23645881..23650290	XP_018822151.1	JrCOL33	364	40.75	5.51	细胞核
JrCOL34	LOC108991312	Chr.11：29101394..29103034	XP_018821038.1	JrCOL34	300	33.48	5.99	细胞核
JrCOL35	LOC108998881	Chr.11：32536877..32538774	XP_018831163.1	JrCOL35	373	40.72	5.86	叶绿体
JrCOL36	LOC109003090	Chr.11：33364576..33368847	XP_018836611.1	JrCOL36	409	45.46	8.94	细胞核
JrCOL37	LOC109005190	Chr.11：36684126..36685787	XP_018839540.1	JrCOL37	341	37.58	6.87	叶绿体
JrCOL38a-b	LOC108982143	Chr.12：8537584..8542475	XP_018808983.1	JrCOL38a	671	74.38	5.97	细胞核
			XP_035539295.1	JrCOL38b	652	72.23	6.08	细胞核
JrCOL39	LOC108992474	Chr.12：13436453..13439714	XP_018822597.1	JrCOL39	237	26.10	4.91	细胞核
JrCOL40a-b	LOC108981163	Chr.12：23463905..23468842	XP_018807797.1	JrCOL40a	423	46.11	4.45	细胞核
			XP_018807806.1	JrCOL40b	418	45.53	4.53	细胞核
JrCOL41a-b	LOC108986927	Chr.13：5686536..5688137	XP_018815283.1	JrCOL41a	293	32.61	5.70	细胞核
			XP_018815285.1	JrCOL41b	206	22.72	5.57	细胞核
JrCOL42	LOC109008345	Chr.13：8537826..8540900	XP_018843947.2	JrCOL42	475	51.86	5.92	细胞核
JrCOL43a-b	LOC108992288	Chr.13：31296667..31301250	XP_018822350.1	JrCOL43a	691	77.57	6.80	细胞核
			XP_018822351.1	JrCOL43b	689	77.43	6.80	细胞核
JrCOL44a-d	LOC109007733	Chr.13：37236625..37242278	XP_018843087.1	JrCOL44a	518	57.78	5.61	细胞核
			XP_018843095.1	JrCOL44b	518	57.78	5.61	细胞核
			XP_018843104.1	JrCOL44c	518	57.78	5.61	细胞核
			XP_018843113.1	JrCOL44d	518	57.78	5.61	细胞核
JrCOL45	LOC109013207	Chr.13：38161618..38165624	XP_018850755.1	JrCOL45	287	32.38	5.25	细胞核
JrCOL46	LOC108996999	Chr.14：6471961..6473893	XP_018828617.1	JrCOL46	218	25.99	5.27	细胞核
JrCOL47	LOC108993945	Chr.14：10350983..10353374	XP_018824556.1	JrCOL47	251	28.23	8.27	细胞核
JrCOL48	LOC109013626	Chr.15：14881124..14883084	XP_018851315.1	JrCOL48	464	52.39	5.09	细胞核,细胞质
JrCOL49	LOC109004589	Chr.16：1780812..1784882	XP_018838732.1	JrCOL49	279	31.45	5.27	细胞核
JrCOL50a-f	LOC109012501	Chr.16：2630759..2636833	XP_035542414.1	JrCOL50a	518	57.90	5.72	细胞核
			XP_035542415.1	JrCOL50b	492	54.89	5.87	细胞核
			XP_035542416.1	JrCOL50c	489	54.51	5.83	细胞核
			XP_035542417.1	JrCOL50d	453	50.39	5.37	细胞核
			XP_018849735.1	JrCOL50e	518	57.90	5.72	细胞核
			XP_018849736.1	JrCOL50f	518	57.90	5.72	细胞核
JrCOL51	LOC109007532	Chr.16：6094342..6099323	XP_018842768.2	JrCOL51	709	78.92	7.28	细胞核
JrCOL52	LOC109008337	Chr.16：22543220..22546364	XP_018843939.1	JrCOL52	475	52.12	6.67	细胞核
JrCOL53a-d	LOC109006537	Chr.16：24449761..24454424	XP_018841385.2	JrCOL53a	209	23.20	6.65	细胞核,细胞质
			XP_035542160.1	JrCOL53b	244	27.19	7.48	细胞质
			XP_035542161.1	JrCOL53c	244	27.19	7.48	细胞质
			XP_035542162.1	JrCOL53d	209	23.20	6.65	细胞核
JrCOL54a-b	LOC109006510	Chr.16：24684660..24686424	XP_018841344.1	JrCOL54a	298	33.48	6.31	细胞核
			XP_018841345.1	JrCOL54b	215	23.72	6.05	细胞核
JrCOL55	LOC108984240	Scaffold_15：88..2233	XP_018811678.1	JrCOL55	170	18.25	4.08	细胞核

基因名称 Gene name	基因ID Gene ID	染色体位置 Chromosome position	相关蛋白 Related protein	蛋白重命名 Protein rename	氨基酸长度 aa length	蛋白分子量 Molecular weight/kD	理论等电点pI	亚细胞定位Subc- ellular localization
JrCOL1	LOC109001350	Chr.1：1256032..1258201	XP_018834143.1	JrCOL1	373	41.31	5.17	叶绿体
JrCOL2	LOC109001381	Chr.1：1488506..1490928	XP_035544637.1	JrCOL2	211	23.40	5.73	细胞质
JrCOL3	LOC109009665	Chr.1：3978995..3983057	XP_018845790.2	JrCOL3	350	39.64	8.72	细胞核
JrCOL4a-d	LOC109010156	Chr.1：18366251..18370500	XP_035543378.1	JrCOL4a	428	47.24	5.27	细胞核
			XP_018846422.1	JrCOL4b	428	47.24	5.27	细胞核
			XP_018846423.1	JrCOL4c	428	47.24	5.27	细胞核
			XP_018846424.1	JrCOL4d	428	47.24	5.27	细胞核
JrCOL5	LOC109015440	Chr.1：18372462..18372860	XP_018853453.2	JrCOL5	132	14.50	6.85	细胞核
JrCOL6a-b	LOC108991987	Chr.1：19317524..19318826	XP_018821963.2	JrCOL6a	237	25.91	4.42	细胞核
			XP_018821964.2	JrCOL6b	236	25.78	4.42	细胞核
JrCOL7a-c	LOC109000761	Chr.1：44957519..44963229	XP_018833298.1	JrCOL7a	416	45.11	5.19	细胞核
			XP_018833299.1	JrCOL7b	416	45.11	5.19	细胞核
			XP_018833303.1	JrCOL7c	416	45.11	5.19	细胞核
JrCOL8	LOC109013302	Chr.2：7141021..7147381	XP_018850883.1	JrCOL8	539	60.48	6.22	细胞核
JrCOL9	LOC108984132	Chr.2：11707792..11709460	XP_018811512.1	JrCOL9	277	31.23	4.94	细胞核
JrCO10	LOC109014521	Chr.2：30680768..30682741	XP_018852564.1	JrCOL10	333	37.49	4.97	细胞核
JrCOL11a-b	LOC109014085	Chr.2：35047335..35048788	XP_018851954.1	JrCOL11a	258	27.76	4.28	细胞核
			XP_018851962.1	JrCOL11b	255	27.37	4.25	细胞核
JrCOL12a-b	LOC108999038	Chr.3：18899526..18901860	XP_018831379.1	JrCOL12a	150	16.21	4.00	细胞核,叶绿体
			XP_035544064.1	JrCOL12b	150	16.21	4.00	细胞核,叶绿体
JrCOL13	LOC109017893	Chr.4：14653613..14655876	XP_035544914.1	JrCOL13	170	18.24	4.11	细胞核
JrCOL14	LOC108989855	Chr.5：6297905..6299253	XP_018819161.1	JrCOL14	220	26.37	4.94	细胞核
JrCOL15a-b	LOC109022307	Chr.5：9188186..9197431	XP_018860727.1	JrCOL15a	789	86.08	6.30	叶绿体
			XP_018860748.1	JrCOL15b	778	84.89	6.35	叶绿体
JrCOL16	LOC108994549	Chr.6：15543376..15545802	XP_018825341.2	JrCOL16	444	48.81	5.88	细胞核
JrCOL17	LOC108998057	Chr.6：29988891..29991120	XP_018830038.2	JrCOL17	407	46.32	5.46	细胞核
JrCOL18	LOC108992642	Chr.7：12544651..12554366	XP_018822785.2	JrCOL18	685	75.72	6.71	细胞核
JrCOL19	LOC108983659	Chr.7：14608063..14609540	XP_018810913.1	JrCOL19	266	28.49	8.73	叶绿体
JrCOL20	LOC109005469	Chr.7：32506953..32517320	XP_018839979.2	JrCOL20	238	26.36	5.08	细胞核
JrCOL21	LOC109019524	Chr.7：38617705..38622031	XP_018857358.1	JrCOL21	432	46.81	4.35	叶绿体
JrCOL22a-b	LOC109014549	Chr.7：49644025..49658252	XP_035548321.1	JrCOL22a	780	85.75	6.42	叶绿体
			XP_035548322.1	JrCOL22b	778	85.50	6.42	叶绿体
JrCOL23	LOC109000996	Chr.8：600112..601757	XP_018833641.1	JrCOL23	345	37.98	6.16	细胞质
JrCOL24	LOC108984911	Chr.8：6608517..6610109	XP_018812557.1	JrCOL24	301	33.04	6.25	细胞核
JrCOL25a-b	LOC108980667	Chr.8：24595513..24597399	XP_035549568.1	JrCOL25a	158	17.12	5.62	细胞质
			XP_035549569.1	JrCOL25b	117	12.99	6.49	细胞质
JrCOL26	LOC108997344	Chr.9：19840907..19842615	XP_018829103.1	JrCOL26	331	37.53	5.83	细胞核
JrCOL27	LOC108985349	Chr.9：21400481..21403221	XP_018813169.1	JrCOL27	295	31.92	5.55	叶绿体
JrCOL28	LOC108986213	Chr.10：2554388..2557653	XP_018814300.1	JrCOL28	299	34.08	6.43	细胞核
JrCOL29a-c	LOC108984383	Chr.10：13263191..13268808	XP_018811858.2	JrCOL29a	436	47.97	6.55	细胞质
			XP_035550266.1	JrCOL29b	436	47.97	6.55	细胞质
			XP_035550267.1	JrCOL29c	408	44.52	5.70	细胞核
JrCOL30a-b	LOC109017472	Chr.10：13911798..13913088	XP_018855279.1	JrCOL30a	260	28.60	4.37	叶绿体
			XP_018855280.1	JrCOL30b	259	28.48	4.37	叶绿体
JrCOL31a-c	LOC109000741	Chr.10：37518173..37524266	XP_018833263.1	JrCOL31a	409	44.45	5.96	细胞核
			XP_018833265.1	JrCOL31b	409	44.45	5.96	细胞核
			XP_018833266.1	JrCOL31c	409	44.45	5.96	细胞核
JrCOL32a-d	LOC108992026	Chr.11：8894066..8897365	XP_018822007.1	JrCOL32a	194	21.42	6.43	叶绿体
			XP_018822008.1	JrCOL32b	192	21.17	6.23	叶绿体
			XP_018822009.1	JrCOL32c	186	20.48	6.43	叶绿体
			XP_018822010.1	JrCOL32d	184	20.22	6.23	叶绿体
JrCOL33	LOC108992144	Chr.11：23645881..23650290	XP_018822151.1	JrCOL33	364	40.75	5.51	细胞核
JrCOL34	LOC108991312	Chr.11：29101394..29103034	XP_018821038.1	JrCOL34	300	33.48	5.99	细胞核
JrCOL35	LOC108998881	Chr.11：32536877..32538774	XP_018831163.1	JrCOL35	373	40.72	5.86	叶绿体
JrCOL36	LOC109003090	Chr.11：33364576..33368847	XP_018836611.1	JrCOL36	409	45.46	8.94	细胞核
JrCOL37	LOC109005190	Chr.11：36684126..36685787	XP_018839540.1	JrCOL37	341	37.58	6.87	叶绿体
JrCOL38a-b	LOC108982143	Chr.12：8537584..8542475	XP_018808983.1	JrCOL38a	671	74.38	5.97	细胞核
			XP_035539295.1	JrCOL38b	652	72.23	6.08	细胞核
JrCOL39	LOC108992474	Chr.12：13436453..13439714	XP_018822597.1	JrCOL39	237	26.10	4.91	细胞核
JrCOL40a-b	LOC108981163	Chr.12：23463905..23468842	XP_018807797.1	JrCOL40a	423	46.11	4.45	细胞核
			XP_018807806.1	JrCOL40b	418	45.53	4.53	细胞核
JrCOL41a-b	LOC108986927	Chr.13：5686536..5688137	XP_018815283.1	JrCOL41a	293	32.61	5.70	细胞核
			XP_018815285.1	JrCOL41b	206	22.72	5.57	细胞核
JrCOL42	LOC109008345	Chr.13：8537826..8540900	XP_018843947.2	JrCOL42	475	51.86	5.92	细胞核
JrCOL43a-b	LOC108992288	Chr.13：31296667..31301250	XP_018822350.1	JrCOL43a	691	77.57	6.80	细胞核
			XP_018822351.1	JrCOL43b	689	77.43	6.80	细胞核
JrCOL44a-d	LOC109007733	Chr.13：37236625..37242278	XP_018843087.1	JrCOL44a	518	57.78	5.61	细胞核
			XP_018843095.1	JrCOL44b	518	57.78	5.61	细胞核
			XP_018843104.1	JrCOL44c	518	57.78	5.61	细胞核
			XP_018843113.1	JrCOL44d	518	57.78	5.61	细胞核
JrCOL45	LOC109013207	Chr.13：38161618..38165624	XP_018850755.1	JrCOL45	287	32.38	5.25	细胞核
JrCOL46	LOC108996999	Chr.14：6471961..6473893	XP_018828617.1	JrCOL46	218	25.99	5.27	细胞核
JrCOL47	LOC108993945	Chr.14：10350983..10353374	XP_018824556.1	JrCOL47	251	28.23	8.27	细胞核
JrCOL48	LOC109013626	Chr.15：14881124..14883084	XP_018851315.1	JrCOL48	464	52.39	5.09	细胞核,细胞质
JrCOL49	LOC109004589	Chr.16：1780812..1784882	XP_018838732.1	JrCOL49	279	31.45	5.27	细胞核
JrCOL50a-f	LOC109012501	Chr.16：2630759..2636833	XP_035542414.1	JrCOL50a	518	57.90	5.72	细胞核
			XP_035542415.1	JrCOL50b	492	54.89	5.87	细胞核
			XP_035542416.1	JrCOL50c	489	54.51	5.83	细胞核
			XP_035542417.1	JrCOL50d	453	50.39	5.37	细胞核
			XP_018849735.1	JrCOL50e	518	57.90	5.72	细胞核
			XP_018849736.1	JrCOL50f	518	57.90	5.72	细胞核
JrCOL51	LOC109007532	Chr.16：6094342..6099323	XP_018842768.2	JrCOL51	709	78.92	7.28	细胞核
JrCOL52	LOC109008337	Chr.16：22543220..22546364	XP_018843939.1	JrCOL52	475	52.12	6.67	细胞核
JrCOL53a-d	LOC109006537	Chr.16：24449761..24454424	XP_018841385.2	JrCOL53a	209	23.20	6.65	细胞核,细胞质
			XP_035542160.1	JrCOL53b	244	27.19	7.48	细胞质
			XP_035542161.1	JrCOL53c	244	27.19	7.48	细胞质
			XP_035542162.1	JrCOL53d	209	23.20	6.65	细胞核
JrCOL54a-b	LOC109006510	Chr.16：24684660..24686424	XP_018841344.1	JrCOL54a	298	33.48	6.31	细胞核
			XP_018841345.1	JrCOL54b	215	23.72	6.05	细胞核
JrCOL55	LOC108984240	Scaffold_15：88..2233	XP_018811678.1	JrCOL55	170	18.25	4.08	细胞核

顺式作用元件 Cis-element	基因数目 Gene number	序列 Sequence	功能 Function	顺式作用元件类型 Cis-element type
ARE	46	AAACCA	厌氧诱导所必需的顺式作用调控元件 cis-elements for anaerobic induction	胁迫相关响应元件 Abiotic and biotic stresses
DRE core	10	GCCGAC	脱水响应元件Dehydration-responsive element	胁迫相关响应元件 Abiotic and biotic stresses
LTR	18	CCGAAA	参与低温反应的顺式作用元件 Low-temperature responsive cis-element
MBS	17	CAACTG	MYB结合位点参与干旱诱导 MYB binding site involved in drought induction
MYB	50	CAACAG	MYB响应元件MYB-responsive element
MYC	53	CAATTG	MYC响应元件MYC-responsive element
STRE	40	AGGGG	胁迫响应元件Stress-responsive element
TC-rich repeat	10	GTTTTCTTAC	参与防御和胁迫反应的顺式作用元素 Defense and stress-responsive element
WUN-motif	21	AAATTACT	创伤响应元件Mechanical wounding element
ABRE	43	ACGTG	脱落酸反应中的顺式作用元件ABA-responsive element	激素相关响应元件 Phytohormone responsive
ERE	32	ATTTTAAA	乙烯响应元件Ethephon-responsive element	激素相关响应元件 Phytohormone responsive
TGA-element	14	AACGAC	生长素响应元件Auxin-responsive element
TCA-element	16	CAGAAAAGGA	参与水杨酸反应的顺式作用元件 Salicylic-acid-responsive element
TGACG-motif	33	TGACG	参与MeJA反应的顺调控元件MeJA-responsive element
CGTCA-motif	34	CGTCA	参与MeJA反应的顺调控元件MeJA-responsive element
P-box	16	CCTTTTG	赤霉素响应元件Gibberellin-responsive element
TATC-box	9	TATCCCA	赤霉素响应元件Gibberellin-responsive element
Box 4	42	ATTAAT	与光反应有关的保守DNA模块的一部分 Part of a conserved DNA module involved in light responsiveness	生长发育相关响应元件 Plant growth and development
CAT-box	13	GCCACT	与分生组织表达相关的调控元件 Involved in Meristem expression element	生长发育相关响应元件 Plant growth and development
GA-motif	9	ATAGATAA	光响应模块的一部分Part of a light-responsive module
GATA-motif	21	AAGGATAAGG	光响应元件的一部分Part of a light-responsive element
G-box	49	CACGTC	参与光响应的调控元件Involved in light-responsive element
GT1-motif	23	GGTTAAT	光响应元件Light-responsive element