生物技术通报 ›› 2022, Vol. 38 ›› Issue (12): 204-213.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0330

• 研究报告 • 上一篇    下一篇

不同发育时期蓝果忍冬果实的转录组分析

孙燕1,2(), 王金刚1(), 臧丹丹2(), 赵恒田2, 刘淑华2   

  1. 1.东北农业大学园艺园林学院,哈尔滨 150030
    2.中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室,哈尔滨 150081
  • 收稿日期:2022-03-19 出版日期:2022-12-26 发布日期:2022-12-29
  • 作者简介:孙燕,女,硕士研究生,研究方向:园林植物与应用;E-mail:yansuns@163.com
  • 基金资助:
    黑龙江省自然科学基金资助项目(YQ2020C030);中国科学院黑土区农业生态重点实验室开放基金项目(2020ZKHT-07)

Transcriptome Analysis of Lonicera caerulea Fruits at Different Developmental Stages

SUN Yan1,2(), WANG Jin-gang1(), ZANG Dan-dan2(), ZHAO Heng-tian2, LIU Shu-hua2   

  1. 1. School of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030
    2. Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081
  • Received:2022-03-19 Published:2022-12-26 Online:2022-12-29

摘要:

为研究不同发育时期(绿熟期和成熟期)蓝果忍冬果实基因的表达差异,探索蓝果忍冬在生长发育过程中的关键代谢通路,以‘中科蓝1号’蓝果忍冬品种为研究对象,利用高通量测序技术对蓝果忍冬绿熟期和成熟期果实的转录组数据进行比较分析。结果表明,共有40.32 Gb Clean Data,每个样品Clean Data均大于6.07 Gb,Q30碱基百分比均在93.89%及以上,GC含量为46.32%-49.38%;两个发育时期的果实共检测出3 247 个差异表达基因,包括1 642 个上调基因和1 605 个下调基因;GO功能富集分析发现,蓝果忍冬果实发育过程中的关键调控基因主要参与代谢、细胞和细胞组分以及催化过程;KEGG富集分析发现,差异表达基因主要富集在植物激素信号转导、碳代谢和氨基酸生物合成等途径。该结果为进一步研究蓝果忍冬果实发育过程中的动态变化提供了参考,为后续开展蓝果忍冬功能基因鉴定、遗传多样性分析等研究提供理论依据。

关键词: 蓝果忍冬, ‘中科蓝1号’, 果实, 转录组, 差异表达基因

Abstract:

In order to investigate the expression differences of Lonicera caerulea fruit genes at different developmental stages(green ripening stage and ripening stage)and to explore the key metabolic pathways of L. caerulea during growth and development,the transcriptome data of L. caerulea at green ripening stage and ripening stage was compared by using high-throughput sequencing technology with ‘Zhongkelan No.1’ variety as the experimental object. The results showed that there were 40.32 Gb Clean Data,each sample had > 6.07 Gb Clean Data,the percentage of Q30 bases was 93.89% and above,and the GC content ranged from 46.32% to 49.38%. There were 3 247 differentially expressed genes detected in the fruits of the two developmental stages,including 1 642 up-regulated genes and 1 605 down-regulated genes. GO functional enrichment analysis revealed that the key regulatory genes during the development of L. caerulea were mainly involved in metabolism,cellular and cellular components and catalytic activity. KEGG enrichment analysis revealed that the differentially expressed genes were mainly enriched in plant hormone signaling,carbon metabolism and amino acid biosynthesis. These results provide a reference for further studies on the dynamic changes during fruit development of L. caerulea,and provide a theoretical basis for subsequent studies on the identification of functional genes and genetic diversity analysis of L. caerulea.

Key words: Lonicera caerulea L., ‘Zhongkelan No.1’, fruit, transcriptome, differentially-expressed gene