Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (8): 77-83.doi: 10.13560/j.cnki.biotech.bull.1985.2016.08.012

• Orignal Article • Previous Articles     Next Articles

Analysis on Fe2+ Stress Resistance of Transgenic Japonica Kongyu 131 with NtFer1

TANG Xin-hua1, JIANG Ting-bo2, GAO Hong-xiu1, WANG Jing-guo1, ZOU De-tang1   

  1. 1. College of Agriculture,Northeast Agricultural University,Harbin 150030;
    2. State Key Laboratory of Forest Genetics and Tree Breeding,Northeast Forestry University,Harbin 150040
  • Revised:2016-01-04 Online:2016-08-25 Published:2016-08-25

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Abstract: In order to realize the gene’s function and improve the resistance ability of japonica,NtFer1 was transformed into japonica Kongyu 131 by Agrobacterium-mediated method. The results by resistance screening,PCR,Southern blotting,Northern blotting,and RT-PCR demonstrated that the gene was integrated into Kongyu 131’s genome,stably and genetically expressed in offspring. T2 generation was cultured in iron deficiency and overload conditions respectively. Under iron deficiency conditions,SOD,POD activity,relative chlorophyll content,total iron content in leaves and roots of transgenic lines were all significantly higher than the control,while the MDA content was lower than the control. Under iron overload conditions,SOD activity,total iron content in leaves and brown rice,and roots growth of transgenic lines were significantly higher than the control,while MDA content and rice blast index were significantly lower than the control. The results revealed that NtFer1 could improve transgenic plant’s ability of oxidative stress and resistance to rice blast,enhance Fe2+ storage ability and stress resistance in iron deficiency and overload conditions.

Key words: NtFer1 gene, japonica, total iron content, oxidative stress