Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (9): 160-165.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0210

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Isolation of Brevibacillus laterosporus A60 and Its Greenhouse Control Efficiency Against Phytophthora capsica

HAO Nan,TONG Zan-Hua,QIU De-Wen   

  1. The Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2017-03-20 Online:2017-09-01 Published:2017-09-15

Abstract: A60 strain with high inhibition rate against Phytophthora capsici was screened from 700 strains isolated from 43 soil samples in Hainan province. The A60 strain was identified as Brevibacillus laterosporus based on physiological and biochemical characteristics and a 16S rDNA gene sequence analysis. The control effects of A60 strain against Phytophthora on Capsicum annuum and Capsicum fructescens were determined in greenhouse. The results showed that the biocontrol efficacy of A60 strain on C. annuum L. were 100%,95.71% and 92.22% at 3rd,5th and 7th day,respectively. The control efficacy on C. fructescens were 100%,75.26% and 73.20% at 3rd,5th and 7th day,respectively. Our results highlight that the application of B. laterosporus A60 significantly reduce morbidity and disease index,i.e.,control efficacy is solid.

Key words: Phytophthora capsici, Brevibacillus laterosporus, biocontrol