Research Advances on Apurinic/Apyrimidinic Endonuclease 1 Mediated Functional Nucleic Acids Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2017-1015

Abstract

Abstract: Apurinic/apyrimidinic Endonuclease 1（APE1）is an enzyme widely existing in living organisms and can effectively and specifically identify and cleave DNA in AP site in the process of base excision repair（BER）. Meanwhile,the activity of APE1 in some tumor cells is significantly higher than that in normal cells,thus it is also a kind of tumor biomarker. At present,by designing AP sites in DNA strand artificially and utilizing the activity of APE1 to produce ideal functional nucleic acids,combined with different methods of signal output and amplification,researchers have established some APE1 mediated electrochemical and fluorescent functional nucleic acids biosensing techniques,by which the detection for the activities of some enzymes such as DNA glycosylase can be conducted. In addition,some functional nucleic acids biosensing techniques for the detection of the activity of APE1 were also established. In this paper we review the research status of APE1 mediated functional nucleic acids biosensing techniques and other biosensing techniques for the detection of APE1,discuss the significances and problems of APE1-relevant biosensing techniques,and prospect development trends of APE1 mediated detecting techniques for the detection of more targets,aiming at promoting APE1 to become a common enzyme tool in functional nucleic acids biosensing techniques.

Key words: AP site, apurinic/apyrimidinic endonuclease 1, functional nucleic acids, biosensor

HE Wan-chong, HUANG Kun-lun, XU Wen-tao. Research Advances on Apurinic/Apyrimidinic Endonuclease 1 Mediated Functional Nucleic Acids Biosensors[J]. Biotechnology Bulletin, 2018, 34(9): 48-54.

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