Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (2): 125-130.doi: 10.13560/j.cnki.biotech.bull.1985.2017.02.018

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Expression and Analysis of an Amylase with Agarase Activity from a Marine Bacterium

LIN Bo-kun1,2, SONG Yan2, LU Guo-yong2, ZHAO Min2, ZHONG Ming-qi2, HU Zhong2   

  1. 1. Dongguan Key Laboratory of Environmental Medicine,School of Public Health,Guangdong Medical University,Dongguan 523808;
    2. Guangdong Provincial Key Laboratory of Marine Biotechnology,Shantou University,Shantou 515063
  • Received:2016-05-19 Online:2017-02-26 Published:2017-02-08

Abstract: Our aim is to confirm that there is amylase with agarase activity in the novel marine bacterial strain ZC1. The amylase gene amy440 of strain ZC1 was cloned and expressed in recombinant prokaryotic vector and the amylase and agarase activity of the recombinant protein was determined using the method of activity staining electrophoresis. Results indicated that the recombinant protein of gene amy440 showed amylase and agarase activity in the activity staining electrophoresis. Conclusively,the encoded protein of gene amy440 of strain ZC1 is an amylase with agarase activity.

Key words: agarase, amylase, multifunctional enzyme, gene cloning, recombinant expression