Screening and Identification of Antagonistic Bacillus spp. Against Cucumber Fusarium wilt and Its Biocontrol Effect

doi:10.13560/j.cnki.biotech.bull.1985.2022-1574

Abstract

Abstract:

Cucumber Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum seriously affects the sustainable development of the cucumber industry. In order to obtain high-efficiency antagonistic Bacillus spp. against cucumber Fusarium wilt and clarify its growth-promoting effect, hte biocontrol strain with a strong antagonistic effect against F. oxysporum were screened from Bacillus with a certain resistance to Fusarium solan, and its morphological, physiological and biochemical, genetic characteristics and plant growth-promoting characteristics were evaluated. The results showed that strain N1、N3 and N6 presented significant inhibitory effects on F. oxysporum, with inhibitory rates of 77.71%, 75.94% and 74.76%, respectively. Three strains were all identified as Bacillus velezensis, all the three strains showed releasing potassium ability, and produced protease, chitinase, pectinase and siderophores. In the seedlings disease-prevention and growth-promotion test, the disease index of inoculated strain N1, N3 and N6 significantly reduced, the control efficiency was 41.93%, 20.97%, and 66.13% respectively on the 18th day after inoculation. Inoculation with N1, N3 and N6 strains effectively promoted the growths of cucumber seedlings compared with single inoculation of F. oxysporum f. sp. treatment, including plant height, stem thickness, leaf area, chlorophyll content and aboveground and subsurface fresh weight, the growth promoting effect of N6 treatment was the most significant. It increased by 21.56%, 56.13%, 47.95%, 76.18%, 222.89% and 70% respectively. Compared with the control, the growth characteristics of treated plants significantly decreased except for the fresh weight of underground parts. Under inoculation F. oxysporum f. sp., the addition of N6 significantly increased the stem thickness and aboveground fresh weight. In conclusion, N6 can be used as potential biocontrol resources to control cucumber Fusarium wilt caused by F. oxysporum f. sp. cucumerinum and promote the growth of seedlings for sustainable and efficient cucumber production.

Key words: biological control, Bacillus velezensis, Fusarium oxysporum f. sp. cucumerinum, cucumber Fusarium wilt, seedling promotion, disease-control effect

CHU Rui, LI Zhao-xuan, ZHANG Xue-qing, YANG Dong-ya, CAO Hang-hang, ZHANG Xue-yan. Screening and Identification of Antagonistic Bacillus spp. Against Cucumber Fusarium wilt and Its Biocontrol Effect[J]. Biotechnology Bulletin, 2023, 39(8): 262-271.

Figures/Tables 10

References 30

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菌株Strain	菌落直径Colony diameter/cm	抑菌率Inhibition rate/%	菌株Strain	菌落直径Colony diameter/cm	抑菌率Inhibition rate/%
N1	2.30	72.18	N26	3.03	60.84
N2	2.40	70.63	N27	3.17	58.78
N3	2.40	70.63	N28	2.79	58.48
N4	2.42	70.38	N29	2.80	58.33
N5	2.43	70.12	N30	2.84	57.58
N6	2.47	69.60	N31	2.84	57.58
N7	2.50	69.09	N32	3.28	56.98
N8	2.58	68.81	N33	2.88	56.82
N9	2.53	68.57	N34	2.93	55.92
N10	2.53	68.57	N35	2.95	55.62
N11	2.53	68.57	N36	3.85	48.22
N12	2.55	68.32	N37	4.11	44.26
N13	2.55	68.32	N38	4.16	43.48
N14	2.60	67.54	N39	4.69	35.24
N15	2.63	67.03	N40	4.27	31.76
N16	2.65	66.77	N41	4.38	29.65
N17	2.68	66.25	N42	5.02	18.18
N18	2.73	65.48	N43	5.40	11.23
N19	2.77	64.97	N44	5.50	9.42
N20	2.80	64.45	N45	5.80	3.99
N21	2.80	64.45	N46	5.80	3.99
N22	2.85	63.68	N47	5.82	3.68
N23	2.87	63.42	N48	5.88	2.48
N24	2.88	63.16	N49	7.10	1.95
N25	2.97	61.88	N50	6.97	0.00

菌株Strain	菌落直径Colony diameter/cm	抑菌率Inhibition rate/%	菌株Strain	菌落直径Colony diameter/cm	抑菌率Inhibition rate/%
N1	2.30	72.18	N26	3.03	60.84
N2	2.40	70.63	N27	3.17	58.78
N3	2.40	70.63	N28	2.79	58.48
N4	2.42	70.38	N29	2.80	58.33
N5	2.43	70.12	N30	2.84	57.58
N6	2.47	69.60	N31	2.84	57.58
N7	2.50	69.09	N32	3.28	56.98
N8	2.58	68.81	N33	2.88	56.82
N9	2.53	68.57	N34	2.93	55.92
N10	2.53	68.57	N35	2.95	55.62
N11	2.53	68.57	N36	3.85	48.22
N12	2.55	68.32	N37	4.11	44.26
N13	2.55	68.32	N38	4.16	43.48
N14	2.60	67.54	N39	4.69	35.24
N15	2.63	67.03	N40	4.27	31.76
N16	2.65	66.77	N41	4.38	29.65
N17	2.68	66.25	N42	5.02	18.18
N18	2.73	65.48	N43	5.40	11.23
N19	2.77	64.97	N44	5.50	9.42
N20	2.80	64.45	N45	5.80	3.99
N21	2.80	64.45	N46	5.80	3.99
N22	2.85	63.68	N47	5.82	3.68
N23	2.87	63.42	N48	5.88	2.48
N24	2.88	63.16	N49	7.10	1.95
N25	2.97	61.88	N50	6.97	0.00

菌株 Strain	菌落直径 Colony diameter/cm	抑菌率 Inhibition rate/%
N1	2.08	77.71
N3	2.20	75.94
N6	2.28	74.76
N7	2.30	74.53
N2	2.32	74.29
N4	2.49	71.82

菌株 Strain	菌落直径 Colony diameter/cm	抑菌率 Inhibition rate/%
N1	2.08	77.71
N3	2.20	75.94
N6	2.28	74.76
N7	2.30	74.53
N2	2.32	74.29
N4	2.49	71.82

试验项目 Test project	结果 Result
试验项目 Test project	N1	N3	N6
甲基红（M-R）Methyl red	-	-	-
接触酶Catalase	-	-	-
氧化酶Oxidase	+	+	+
淀粉水解Amylum hydrolysis	-	-	-
纤维素酶Cellulase	-	-	-
明胶液化Gelatin experiment	+	-	+
乙酰甲基甲醇（V-P）Voges-Proskauer	-	-	-
葡萄糖氧化发酵Oxidation-fermentation of glucose	+	+	+